Recombinant Human LRIT2 Protein, CF

Recombinant Human LRIT2 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: LRIT2

Leucine-rich Repeat, Immunoglobulin-like domain and Transmembrane domain-containing protein 2 (LRIT2), also known as leucine-rich repeat protein 22 (LRRC22), is a 550 amino acid (aa) type I transmembrane protein. Its extracellular domain (ECD) contains one LRRNT, LRRCT, Ig-like, and Fibronectin type-III domain, and four leucine-rich repeats (LRRs). Within the ECD, human LRIT2 shares approximately 75% aa sequence identity with the mouse and rat orthologs. The LRR is a structural motif consisting of 20 to 30 aa repeated 2 to 45 times that forms a hydrophobic horseshoe shape with a parallel beta  sheet on the concave side and helical elements on the convex side (1-4). All LRRs are composed of a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif (1-4). LRR motifs mediate protein-protein interactions in a variety of proteins including tyrosine kinase receptors, hormone receptors, extracellular matrix-binding proteins, cell-adhesion molecules, and bacterial virulence factors (1, 3). Proteins containing LRRs play important roles in many biologically processes such as mammalian and neural development, cell polarization, gene expression, and apoptosis (2).