Recombinant Human LRIT2 Protein, CF

R&D Systems | Catalog # 8388-LR

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human LRIT2 Protein (8388-LR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

A6NDA9

Applications

Bioactivity
View all LRIT2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LRIT2 protein
Gln21-His462 (Leu220Phe) with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln21

Predicted Molecular Mass

49 kDa

SDS-PAGE

67-81 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells.
The ED50 for this effect is 0.6-3 μg/mL.

Formulation, Preparation, and Storage

8388-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRIT2

Leucine-rich Repeat, Immunoglobulin-like domain and Transmembrane domain-containing protein 2 (LRIT2), also known as leucine-rich repeat protein 22 (LRRC22), is a 550 amino acid (aa) type I transmembrane protein. Its extracellular domain (ECD) contains one LRRNT, LRRCT, Ig-like, and Fibronectin type-III domain, and four leucine-rich repeats (LRRs). Within the ECD, human LRIT2 shares approximately 75% aa sequence identity with the mouse and rat orthologs. The LRR is a structural motif consisting of 20 to 30 aa repeated 2 to 45 times that forms a hydrophobic horseshoe shape with a parallel beta  sheet on the concave side and helical elements on the convex side (1-4). All LRRs are composed of a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif (1-4). LRR motifs mediate protein-protein interactions in a variety of proteins including tyrosine kinase receptors, hormone receptors, extracellular matrix-binding proteins, cell-adhesion molecules, and bacterial virulence factors (1, 3). Proteins containing LRRs play important roles in many biologically processes such as mammalian and neural development, cell polarization, gene expression, and apoptosis (2).

References

  1. Kobe, B. and J. Deisenhofer (1994) Trends Biochem. Sci. 19:415.
  2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725.
  3. Enkhbayar, P. et al. (2004) Proteins 54:394.
  4. Bella, J. et al. (2008) Cell. Mol. Life Sci. 65:2307.

Long Name

Leucine-Rich Repeat, Immunoglobulin-Like And Transmembrane Domains 2

Alternate Names

LRRC22, PAL2

Entrez Gene IDs

340745 (Human); 239038 (Mouse)

Gene Symbol

LRIT2

UniProt

A6NDA9

Additional LRIT2 Products

Product Documents for Recombinant Human LRIT2 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human LRIT2 Protein, CF

For research use only

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