Recombinant Human LRRN4 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10227-LR-050
Recombinant Human LRRN4 His-tag Protein, CF SDS-PAGE
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Recombinant Human LRRN4 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human LRRN4 His-tag (Catalog # 10227-LR), immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived human LRRN4 protein
Asp19-Ser679, with a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
71 kDa
SDS-PAGE
106-123 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10227-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human LRRN4 His-tag (Catalog # 10227-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-123 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRRN4

LRRN4 (Leucine-rich repeat neuronal protein 4) is a type I transmembrane protein that is a member of the LRRN family. It is expressed in lung, heart, ovary, and neuronal tissues (1-3). Mature human LRRN4 is composed of a 661 amino acid (aa) extracellular domain (ECD) that includes 10 LRRs and a fibronectin type III-like domain, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, human LRRN4 shares 66% and 64% aa sequence identity with mouse and rat LRRN4, respectively. LRRN-4-deficient mice show defects in the memory retention, suggesting this protein may be involved in memory retention (4). In addition, LRRN4 is found in about 8% dorsal root ganglion (DEG) neurons (2). These neurons are small-sized neurons that function as nociceptors. LRRN4 expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4 might function as a synaptic adhesion molecule to maintain nociceptive circuits (2). LRRN4 is also expressed in primary mesothelial cells and may be developed as a marker for detection of mesothelioma antigens (5).

References
  1. Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
  2. Bando, T. et al. (2012) Neurosci. Lett. 531:24.
  3. Bando, T. et al. (2013) Neurosci. Lett. 548:73.
  4. Bando, T. et al. (1995) Mol. Cellular. Biol. 25:4166.
  5. Kanamori-Katayama, M. (2011) PloS. One. 6:e24391.
Long Name
Leucine-rich Repeat Neuronal 4
Entrez Gene IDs
164312 (Human); 320974 (Mouse); 311443 (Rat)
Alternate Names
C20orf75; chromosome 20 open reading frame 75; dJ1056H1.1; FLJ23994; leucine rich repeat neuronal 4; leucine-rich repeat neuronal protein 4; LRRN4; MGC25027; Neuronal leucine-rich repeat protein 4; NLRR4; NLRR-4

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