Recombinant Human LRRTM2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5589-LR-050
Recombinant Human LRRTM2 Fc Chimera Protein, CF Binding Activity
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Recombinant Human LRRTM2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Neurexin 1 alpha (-S4) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human LRRTM2 Fc Chimera that produces 50% of the optimal binding response is 0.07-0.42 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human LRRTM2 protein
Human LRRTM2
(Cys34-Arg422)
Accession # O43300
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Cys34
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
71 kDa
SDS-PAGE
83-100 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5589-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Rat Neurexin 1 alpha (-S4) is coated at 1 μg/mL (100 μL/well),  Recombinant Human LRRTM-2 Fc Chimera (Catalog # 5589-LR) binds with an ED50 of 0.07‑0.42 μg/mL

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human LRRTM2 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 83-100 kDa and 160-200 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRRTM2

LRRTM2 (Leucine-rich repeat transmembrane protein 2) is a member of the LRRTM family of molecules (1). All LRRTMs are type I transmembrane proteins that contain multiple leucine rich repeats and one PDZ consensus cytoplasmic binding domain. The LRRTM family is expressed in the central nervous system across vertebrate species, and they are not found in invertebrates (1). Human LRRTM2 is synthesized as a 516 amino acid (aa) precursor that contains a 33 aa signal sequence, a 389 aa extracellular domain, a 21 aa transmembrane segment, and a 73 aa cytoplasmic region (1). The extracellular domain is characterized by the presence of ten Leucine-rich repeats, flanked by two cysteine-rich sequences. Mature human LRRTM2 is 98% aa identical to mouse LRRTM2 (1). LRRTM2 functions as a postsynaptic organizer in excitatory synapses. LRRTM2 binds only Neurexin-alpha and Neurexin-beta which are lacking splice site 4 (S4) (2). Crystal structure shows dependence of this interaction on Calcium ion as well as overlapping binding interface with Neuroligins (3). LRRTM2 is essential for long term potentiation in hippocampal neuron by maintaining AMPA Receptors at the synapse (4).

References
  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Ko, J. et al. (2009) Neuron 64:791.
  3. Yamagata, A. et al. (2018) Nat. Commun. 9:3964.
  4. Soler-Llavina, G.J. et al. (2013) Neuron 79:439.
Long Name
Leucine Rich Repeat Transmembrane Neuronal 2
Entrez Gene IDs
26045 (Human); 107065 (Mouse); 685472 (Rat)
Alternate Names
leucine-rich repeat transmembrane neuronal protein 2; leucine rich repeat transmembrane neuronal 2; LRRN2; LRRTM2

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