Recombinant Human LRRTM2 Fc Chimera Protein, CF Summary
Accession # O43300
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Rat Neurexin 1 alpha (-S4) is coated at 1 μg/mL (100 μL/well), Recombinant Human LRRTM-2 Fc Chimera (Catalog # 5589-LR) binds with an ED50 of 0.07‑0.42 μg/mL
2 μg/lane of Recombinant Human LRRTM2 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 83-100 kDa and 160-200 kDa, respectively.
LRRTM2 (Leucine-rich repeat transmembrane protein 2) is a member of the LRRTM family of molecules (1). All LRRTMs are type I transmembrane proteins that contain multiple leucine rich repeats and one PDZ consensus cytoplasmic binding domain. The LRRTM family is expressed in the central nervous system across vertebrate species, and they are not found in invertebrates (1). Human LRRTM2 is synthesized as a 516 amino acid (aa) precursor that contains a 33 aa signal sequence, a 389 aa extracellular domain, a 21 aa transmembrane segment, and a 73 aa cytoplasmic region (1). The extracellular domain is characterized by the presence of ten Leucine-rich repeats, flanked by two cysteine-rich sequences. Mature human LRRTM2 is 98% aa identical to mouse LRRTM2 (1). LRRTM2 functions as a postsynaptic organizer in excitatory synapses. LRRTM2 binds only Neurexin-alpha and Neurexin-beta which are lacking splice site 4 (S4) (2). Crystal structure shows dependence of this interaction on Calcium ion as well as overlapping binding interface with Neuroligins (3). LRRTM2 is essential for long term potentiation in hippocampal neuron by maintaining AMPA Receptors at the synapse (4).
- Lauren, J. et al. (2003) Genomics 81:411.
- Ko, J. et al. (2009) Neuron 64:791.
- Yamagata, A. et al. (2018) Nat. Commun. 9:3964.
- Soler-Llavina, G.J. et al. (2013) Neuron 79:439.
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