Recombinant Human Ly6E Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9970-L6-050
Recombinant Human Ly6E Fc Chimera Protein, CF Bioactivity
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Recombinant Human Ly6E Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 1-7.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Ly6E protein
Human LY6E
(Leu21-Ser101)
Accession # Q16553
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
35 kDa
SDS-PAGE
37-47 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9970-L6

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human Ly6E Fc Chimera (Catalog # 9970-L6) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 1-7.5 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Ly6E Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 37-47 kDa and 70-90 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ly6E

Ly6E (Lymphocyte antigen 6E), also known as Stem cell antigen 2 (SCA-2) or Thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 protein superfamily (1, 2). There are at least twenty different human Ly6 proteins that have been identified, ranging from 11 to 36 kDa. These are classified as either secreted, or membrane-bound proteins which have the GPI-anchor signaling sequence. Included in the GPI-anchored cell surface glycoproteins are Ly6A-I and Ly6K (2-4). Human Ly6E is synthesized as a 131 amino acid (aa) protein that includes a 20 aa signal peptide, an 81 aa Ly6E chain, and a 30 aa propeptide. Within the main chain region, human Ly6E shares 55% and 57% aa sequence identity with mouse and rat Ly6E, respectively. Ly6E is involved in cell signalling transduction, cell adhesion, immune regulation and drug resistance, and is over-expressed in human malignancies, including head and neck squamous cell carcinomas and lung, and oesophageal cancers (4-7). Moreover, Ly6E over-expresses in gastric cancer cells, and is important for cell survival, proliferation and migration, and could be a novel oncogenic protein for efficient diagnosis and treatment of gastric cancer (2). LY6E has recently been shown to promote viral entry of some flaviviruses, including West Nile virus, Dengue virus, and Zika virus (8).

References
  1. Gumley, T.P. et al. (1995) Immunol. Cell Biol. 73:277.
  2. Lv, Y. et al. (2018) Cell. Physiol. Biochem. 45:1219.
  3. Kong, H.K. and J.H. Park. (2012) BMB Rep. 45:595.
  4. Luo, L. et al. (2016) Oncotarget 7:11165.
  5. Yeom, C.J. et al. (2016) Oncotarget 7:65837.
  6. AlHossiny M. et al. (2016) Cancer Res.76:3376.
  7. Asundi J. et al. (2015) Clin Cancer Res. 21:3252.
  8. Mar K.B. et al. (2018) Nat Commun. 9:3603.
Long Name
Lymphocyte Antigen 6 Family Member E
Entrez Gene IDs
4061 (Human); 17069 (Mouse); 362934 (Rat)
Alternate Names
Ly6E; Ly-6E; lymphocyte antigen 6 complex, locus E; Retinoic acid-induced gene E protein; RIGE; RIGE9804; RIG-Ely-6E; SCA2; SCA2Ly-6E; SCA-2lymphocyte antigen 6E; Stem cell antigen 2; Thymic shared antigen 1; TSA1; TSA-1retinoic acid induced gene E

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