Recombinant Human Lymphotoxin-alpha/TNF-beta Protein, CF

Name: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein, CF
Brand: R&D Systems
SKU: 211-TBB/CF
Price: 315 USD
Availability: InStock

R&D Systems | Catalog # 211-TBB/CF

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Citations (9)

Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Accession Number

P01374

Applications

Bioactivity

View all Lymphotoxin-alpha/TNF-beta Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Lymphotoxin-alpha/TNF-beta protein
Leu35-Leu205, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

19 kDa

SDS-PAGE

17-19 kDa, reducing conditions

Activity

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED₅₀ for this effect is 4-20 pg/mL.

Scientific Data Images for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein, CF

Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Bioactivity

Recombinant Human Lymphotoxin-alpha /TNF-beta (Catalog # 211-TBB/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 4-20 pg/mL.

Recombinant Human Lymphotoxin-alpha/TNF-beta Protein SDS-PAGE

1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 211-TBB/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 211-TBB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Lymphotoxin-alpha/TNF-beta

Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta (1-3). Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14 (4). LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3 (4). In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function (5-8). LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease (9, 10).