Recombinant Human MAN2B1 Protein, CF
R&D Systems | Catalog # 9826-GH
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Key Product Details
- R&D Systems HEK293-derived Recombinant Human MAN2B1 Protein (9826-GH)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
HEK293
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human MAN2B1 protein
Gly50-Gly1011, with a C-terminal 6-His tag
Gly50-Gly1011, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly50
Predicted Molecular Mass
110 kDa
SDS-PAGE
109-127 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-mannopyranoside.
The specific activity is >1,500 pmol/min/μg, as measured under the described conditions.
The specific activity is >1,500 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
9826-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MAN2B1
Long Name
Mannosidase alpha, Class 2B, Member 1
Alternate Names
LAMAN, MANB, Mannosidase Alpha-B
Gene Symbol
MAN2B1
UniProt
Additional MAN2B1 Products
Product Documents for Recombinant Human MAN2B1 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Manufacturing Specifications
- Aronson, N.N. Jr. and Kuranda, M.J. (1989) FASEB J. 3:2615.
- Liao, Y.F. et al. (1996) J. Biol. Chem. 271:28348.
- Gotoda, Y. et al. (1989) Am. J. Hum. Genet. 63:1015.
- Nebes, V.L. and Schmidt, M.C. (1994) Biochem. Biophys. Res. Commun. 200:239.
- Nilssen, O, et al. (1997) Hum. Mol. Genet. 6:717.
- Yu, P. et al. (2015) Genet. Med. 17:971.
Product Specific Notices for Recombinant Human MAN2B1 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human MAN2B1 Protein, CF (9826-GH):
Materials
- Assay Buffer: 50 mM Sodium Acetate, pH 4.5
- Recombinant Human MAN2B1 (rhMAN2B1) (Catalog # 9826-GH)
- Substrate: 4-Methylumbelliferyl-alpha -D-mannopyranoside (Sigma, Catalog # M3657), 50 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhMAN2B1 to 2 ng/μL in Assay Buffer.
- Dilute Substrate to 400 µM in Assay Buffer.
- Load 50 μL of 2 ng/μL rhMAN2B1 into the plate, and start the reaction by adding 50 μL of 400 µM Substrate. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of 400 µM Substrate.
- Read at excitation and emission wavelengths of 365 nm and 445 nm, respectively, (top read) in kinetic mode for 5 minutes.
- Calculate specific activity:
Specific Activity (pmol/min/µg) = | Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank.
**Derived using calibration standard 4-Methylumbelliferone (Sigma, Catalog # M1381).
- rhMAN2B1: 0.1 µg
- Substrate: 200 µM