Recombinant Human MANF Protein, CF

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to support the survival and stimulate neurite outgrowth of rat embryonic cortical neurons. The ED50 for this effect is 0.7-2.8 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived human MANF protein
    Leu22-Leu179 with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu22
  • Predicted Molecular Mass
    19.0 kDa
  • SDS-PAGE
    20.4 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

3748-MN
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 200 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: MANF
Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as arginine-rich, mutated in early stage tumors (ARMET) and arginine-rich protein (ARP), is a 20 kDa member of the ARMET family of proteins (1). The name ARMET comes from the fact that the protein was initially thought to be 50 aa longer at the N-terminus and to contain an arginine-rich region (2-5). The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors (2-4).  Human MANF is synthesized as a 179 amino acid (aa) precursor that contains a 21 aa signal sequence and a 158 aa mature chain. Mature human MANF is 99%, 98% and 96% aa identical to mature rat, mouse and bovine MANF, respectively. MANF is localized to the endoplasmic reticulum (ER) and Golgi apparatus, and is also secreted (5). In the CNS, MANF selectively protects nigral dopaminergic neurons, versus GABAergic or serotonergic neurons, which suggests that MANF may be indicated for the treatment of Parkinson’s disease (1). MANF is also one of the 12 commonly unfolded protein response (UPR)-up-regulated genes (5). One study showed that MANF plays an important role in protecting cells against tunicamycin and thapsigargin-induced cell death (5). Loss of MANF renders cells more susceptible to those drugs, but also increases cell proliferation and decreases cell size (5). Another study showed that MANF is an endoplasmic reticulum stress response (ERSR) gene in the heart that can be induced and secreted in response to ER stresses, including ischemia, and that extracellular MANF may protect cardiac myocytes in an autocrine and paracrine manner (6).
  • References:
    1. Petrova, P.S. et al. (2003) J. Mol. Neurosci. 20:173.
    2. Shridhar, V. et al. (1996) Oncogene 12:1931.
    3. Shridhar, R. et al. (1996) Cancer Res. 56:5576.
    4. Shridhar, V. et al. (1997) Oncogene 14:2213.
    5. Apostolou, A. et al. (2008) Exp. Cell Res. 314:2454.
    6. Tadimalla, A. et al. (2008) Circ. Res. 103:1249.
  • Long Name:
    Mesencephalic Astrocyte-derived Neurotrphic Factor
  • Entrez Gene IDs:
    7873 (Human); 74840 (Mouse); 315989 (Rat)
  • Alternate Names:
    Arginine-Rich Protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; MANF; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB , Flow , AP MAB050R 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human MANF Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee