Recombinant Human MCEMP1 Fc Chimera Protein, CF

Catalog #: 4994-MC Datasheet / COA / SDS

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4994-MC-050

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Recombinant Human MCEMP1 Fc Chimera Protein, CF Summary

R&D Systems NS0-derived Recombinant Human MCEMP1 Fc Chimera Protein (4994-MC)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.15-0.75 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived human MCEMP1 protein

Met-Asp	Human IgG₁ (Pro100-Lys330)	IEGR	Human MCEMP1 (Val106-Gln187) Accession # NP_777578
N-terminus			C-terminus

Accession #

NP_777578

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

36 kDa (monomer)

SDS-PAGE

39-43 kDa, reducing conditions

Product Datasheets

4994-MC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4994-MC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MCEMP1

MCEMP1 (mast cell‑expressed membrane protein-1), or C19orf59 in humans, is a 22 kDa mast cell specific type II transmembrane protein expressed in lung (1). Human MCEMP1 cDNA encodes 187 amino acids (aa) including an 85 aa N‑terminal cytoplasmic region, a 21 aa transmembrane domain, and an 81 aa C‑terminal extracellular domain (ECD, aa 107‑187) that includes one potential N-glycosylation site. One potential 204 aa splice variant contains a 22 aa substitution for the C‑terminal five amino acids. Within the ECD, human MCEMP1 shares only 36‑44% aa sequence identity with presumed mouse, rat, canine, porcine and bovine MCEMP1 orthologs (1). The 5’ flanking sequence of human MCEMP1 contains motifs for NF kappa B/Rel and NFAT, indicating that MCEMP1 expression may be enhanced by stimuli that also enhance cytokine production, such as allergic reactions or pathogens (2). In addition to lung mast cells, protein expression of MCEMP1 has been identified in cord blood-derived cultured mast cells, the mast cell leukemia cell line HMC-1, the acute monocytic leukemia cell line THP-1, and primary CD14⁺ monocytes and mast cells within or adjacent to lung tumors (1). It is not found on peripheral blood lymphocytes, lymphocytic cell lines, or epithelial cells (1).

References