Recombinant Human MDH1 His-tag Protein, CF

R&D Systems | Catalog # 11632-MH

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human MDH1 His-tag Protein (11632-MH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P40925.4

Applications

Enzyme Activity
View all MDH1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human MDH1 protein
Met1-Ala334, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1 & Ser2

Predicted Molecular Mass

37 kDa

SDS-PAGE

35-38 kDa, under reducing conditions

Activity

Measured by its ability to produce malate from oxaloacetate.
The specific activity is >55,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human MDH1 His-tag Protein, CF

Recombinant Human MDH1 His-tag Enzyme Activity.

Recombinant Human MDH1 His-tag (Catalog # 11632-MH) is measured by its ability to produce malate from oxaloacetate.

Recombinant Human MDH1 His-tag SDS-PAGE.

2 μg/lane of Recombinant Human MDH1 His-tag (Catalog # 11632-MH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-38 kDa, under reducing conditions.

Formulation, Preparation, and Storage

11632-MH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl. 
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MDH1

Malate Dehydrogenase 1 (MDH1), also known as aromatic alpha-keto acid reductase, belongs to the family of nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenases and is a key enzyme in the malate-aspartate shuttle where it plays a critical role in the regulation of metabolic activity in humans (1, 2). MDH1 is an active homodimer of monomers that each contain the characteristic conserved Rossman fold, similar NAD binding sites, and dimeric quaternary structure of MDH enzymes (3). MDH is ubiquitously expressed in cells in two separate isoforms that differ in cellular localization and share only 26% sequence homology (3). While MDH2 is a mitochondrial form involved in the regulation of mitochondrial NAD levels within the citric acid cycle, MDH1, the cytosolic form, plays an important role in the regulation of cytosolic NAD levels. Increased cytosolic NAD levels are necessary for maintaining enhanced glycolysis of proliferating cancer cells (4) and MDH1 is overexpressed in tumors and correlated with poor prognosis (5-8).  In addition, MDH1 has been found to serve as a potential marker in several diseases with inflammation and deficiency of MDH1 is associated with early onset severe encephalopathy and associated with acute liver failure (2, 9-11) making it a metabolic therapeutic target of interest with several potential applications (1, 3, 6, 12, 13).  

References

  1. Godesi, S. et al. (2023) Pharmaceuticals 16:683.
  2. Khamis, A.A. et al. (2024). Genes Nutr. 19:20.
  3. McCue, W.M and B.C Finzel (2022) ASC Omega 7:207.
  4. Lunt, S.Y. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:441.
  5. Wang, Y.P. et al. (2016) Mol. Cell. 64:673.
  6. Zhang, B. et al. (2017) J. Cancer 8:2088.
  7. Sun, W. et al. (2024) BMC Cancer. 24:905.
  8. Wang, J. et al. (2024) Clin. Transl. Med. 14:e1680.
  9. Broeks, M.H. et al. (2019) Hum. Genet. 138:1247.
  10. Shi, C. et al. (2024) iScience. 27:109678.
  11. Zeng, X. et al. (2024) Mol. Neurodegener. 19:68.
  12. Hanse, E. et al. (2017) Oncogene 36:3915.
  13. Naik, R. et al. (2017) J. Med. Chem. 60:8631.

Long Name

malate dehydrogenase 1

Alternate Names

DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2

Entrez Gene IDs

4190 (Human); 17449 (Mouse); 24551 (Rat)

Gene Symbol

MDH1

UniProt

P40925.4

Additional MDH1 Products

Product Documents for Recombinant Human MDH1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human MDH1 His-tag Protein, CF

For research use only

Customer Reviews for Recombinant Human MDH1 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human MDH1 His-tag Protein, CF and earn rewards!

Have you used Recombinant Human MDH1 His-tag Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human MDH1 His-tag Protein, CF (11632-MH):

Materials
  • Assay Buffer: 50 mM Tris, 100 mM NaCl, pH 9.0
  • Recombinant Human Malate Dehydrogenase 1 (rhMDH1) (Catalog # 11632-MH)
  • beta -Nicotinamide adenine dinucleotide, reduced disodium salt hydrate (NADH), 20 mM stock in 0.1 M Sodium Borate, pH 9.0
  • Oxaloacetic Acid, 100 mM Stock in deionized water
  • Clear 96-Well Plate 
  • Plate Reader with Absorbance Read Capability
  1. Dilute rhMDH1 to 0.5 µg/mL in Assay Buffer.
  2. Prepare a substrate mixture containing 1.6 mM NADH and 2 mM Oxaloacetic Acid in Assay Buffer.
  3. In a plate, load 50 µL of 0.5 µg/mL rhMDH1 and start the reaction by adding 50 µL of substrate mixture. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of substrate mixture. 
  4. Read plate at 340 nm (absorbance) in kinetic mode for 10 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol x (-1)
  epsilon ** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

    

*Adjusted for Substrate Blank
**Using the extinction coefficient 6220 M-1cm-1 
***Using the path correction of 0.320 cm
Per Well:
  • rhMDH1: 0.025 µg
  • NADH: 0.8 mM 
  • Oxaloacetic Acid: 1 mM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes