Recombinant Human MDL-1/CLEC5A Protein, CF

Catalog # Availability Size / Price Qty
8544-CL-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human MDL-1/CLEC5A Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin‑9 (Catalog # 2045-GA) is coated at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human MDL-1/CLEC5A that produces 50% optimal binding response is typically 3-18 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human MDL-1/CLEC5A protein
Tyr26-Lys188, with an N-terminal 9-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
20 kDa
SDS-PAGE
34-45 kDa, reducing conditions

Product Datasheets

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8544-CL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8544-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: MDL-1/CLEC5A

MDL-1 (Myeloid DAP12-associating lectin 1), also known as CLEC5A, is an approximately 40 kDa member of the C-type lectin family (1). Mature human MDL-1 is a glycosylated type 2 transmembrane protein that associates into a homodimer on the cell surface (2). It consists of a 161 amino acid (aa) extracellular domain (ECD) with one C-type lectin (CTL) domain and a juxtamembrane stalk region, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (3). Within the ECD, human MDL-1 shares 67% and 69% aa sequence identity with mouse and rat MDL-1, respectively. The transmembrane segment contains a lysine residue that mediates interactions with the signaling protein DAP12 (3). MDL-1 is expressed on monocytes, macrophages, and neutrophils (3, 4). Its expression is up-regulated on monocytes in rheumatoid arthritis (5). MDL-1 functions as a cell attachment receptor for all four serotypes of Denguevirus as well as Japanese encephalitis virus, although the short isoform binds significantly more weakly (2, 6, 7). These interactions trigger DAP12 phosphorylation, the production of multiple inflammatory cytokines, vascular leakage, and disruption of the blood-brain barrier (6-8).

References
  1. Hoving, J.C. et al. (2014) Cell. Microbiol. 16:185.
  2. Watson, A.A. et al. (2011) J. Biol. Chem. 286:24208.
  3. Bakker, A.B.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9792.
  4. Aoki, N. et al. (2009) J. Leukoc. Biol. 85:508.
  5. Chen, D.-Y. et al. (2014) PLoS ONE 9:e86105.
  6. Chen, S.-T. et al. (2012) PLoS Pathogens 8:e1002655.
  7. Chen, S.-T. et al. (2008) Nature 453:672.
  8. Wu, M.-F. et al. (2013) Blood 121:95.
Long Name
Myeloid DAP12-associating Lectin
Entrez Gene IDs
23601 (Human); 23845 (Mouse)
Alternate Names
CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1

Citation for Recombinant Human MDL-1/CLEC5A Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CLEC5A mediates Zika virus-induced testicular damage
    Authors: HW Wang, HH Li, SC Wu, CK Tang, HY Yu, YC Chang, PS Sung, WL Liu, MP Su, GY Yu, LR Huang, CH Chen, SL Hsieh
    Journal of Biomedical Science, 2023-02-17;30(1):12.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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