Recombinant Human Melanotransferrin/CD228 His Protein, CF

R&D Systems | Catalog # 10696-MT

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Melanotransferrin/CD228 His Protein (10696-MT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_005920.2

Applications

Bioactivity
View all Melanotransferrin/CD228 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Melanotransferrin/CD228 protein
Gly20-Gly711, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

76 kDa

SDS-PAGE

84-92 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Melanotransferrin/CD228 His-tag (Catalog # 10696-MT) binds Human Melanotransferrin/CD228 Antibody (Catalog # MAB81751) with an ED50 of < 400 ng/mL.

Scientific Data Images for Recombinant Human Melanotransferrin/CD228 His Protein, CF

Recombinant Human Melanotransferrin/CD228 His-tag Protein SDS PAGE

2 μg/lane of Recombinant Human Melanotransferrin/CD228 His-tag Protein (Catalog # 10696-MT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-92 kDa and 75-85 kDa, respectively.

Formulation, Preparation, and Storage

10696-MT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 µg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Melanotransferrin/CD228

Melanotransferrin (MTF), also known as CD228, P97, MELTF, or MFI2, is a member of the transferrin superfamily that binds to a single ferric iron with high affinity (1). It was originally identified as a membrane-bound protein with a coding region of 2217 bases encoding 738 amino acids (aa) including five Fe-binding residues, the consensus thermolysin metalloprotease sites, and a glycosyl-phosphatidylinositol (GPI) anchor (2). A secreted form of MTF (sMTF) has also been reported (2, 3). Human MTF shares 86% aa sequence identity with mouse and rat MTF. MTF expression is low in normal tissues but high in tumor and embryonic tissues. In melanoma MTF is associated with tumor metastasis and angiogenesis and upregulated in lung cancer tissue and cell lines (3). In the brain MTF is expressed in capillary endothelium but also in the reactive microglia associated with senile plaques in Alzheimer's disease (AD) (4). sMTF has been reported to be increased in patients with AD or arthritis and may modulate angiogenesis, cell migration, and plasminogen activation (5).

References

  1. Baker, E.N. et al. (1992) FEBS Lett. 298:215.
  2. Sekyere, E. & D.R. Richardson (2000) FEBS Lett. 483:11.
  3. Lei, Y. et al. (2020) Cell Death Dis. 11:933.
  4. Jefferies, W.A. et al. (1996) Brain Res. 712:122.
  5. Paluncic, J. et al. (2016) Biochim. Biophy. Acta. 1863:770.

Alternate Names

CD228, MAP97, MFI2, MTF, p97

Entrez Gene IDs

4241 (Human); 30060 (Mouse); 288038 (Rat)

Gene Symbol

MELTF

UniProt

NP_005920.2

Additional Melanotransferrin/CD228 Products

Product Documents for Recombinant Human Melanotransferrin/CD228 His Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Melanotransferrin/CD228 His Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers

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