Recombinant Human Melanotransferrin/CD228 His Protein, CF

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Recombinant Human Melanotransferrin/CD228 His-tag Protein SDS PAGE
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Product Details

Recombinant Human Melanotransferrin/CD228 His Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Melanotransferrin/CD228 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Human embryonic kidney cell, HEK293-derived human Melanotransferrin/CD228 protein
Gly20-Gly711, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
76 kDa
84-92 kDa, under reducing conditions

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Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Melanotransferrin/CD228 His-tag Protein (Catalog # 10696-MT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-92 kDa and 75-85 kDa, respectively.

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Background: Melanotransferrin/CD228

Melanotransferrin (MTF), also known as CD228, P97, MELTF, or MFI2, is a member of the transferrin superfamily that binds to a single ferric iron with high affinity (1). It was originally identified as a membrane-bound protein with a coding region of 2217 bases encoding 738 amino acids (aa) including five Fe-binding residues, the consensus thermolysin metalloprotease sites, and a glycosyl-phosphatidylinositol (GPI) anchor (2). A secreted form of MTF (sMTF) has also been reported (2, 3). Human MTF shares 86% aa sequence identity with mouse and rat MTF. MTF expression is low in normal tissues but high in tumor and embryonic tissues. In melanoma MTF is associated with tumor metastasis and angiogenesis and upregulated in lung cancer tissue and cell lines (3). In the brain MTF is expressed in capillary endothelium but also in the reactive microglia associated with senile plaques in Alzheimer's disease (AD) (4). sMTF has been reported to be increased in patients with AD or arthritis and may modulate angiogenesis, cell migration, and plasminogen activation (5).

  1. Baker, E.N. et al. (1992) FEBS Lett. 298:215.
  2. Sekyere, E. & D.R. Richardson (2000) FEBS Lett. 483:11.
  3. Lei, Y. et al. (2020) Cell Death Dis. 11:933.
  4. Jefferies, W.A. et al. (1996) Brain Res. 712:122.
  5. Paluncic, J. et al. (2016) Biochim. Biophy. Acta. 1863:770.
Entrez Gene IDs
4241 (Human); 30060 (Mouse); 288038 (Rat)
Alternate Names
antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5; CD228 antigen; CD228; FLJ38863; MAP97; MAP97Melanoma-associated antigen p97; Melanotransferrin; membrane-bound transferrin-like protein; MFI2; MGC4856; MTF; MTF1; p97


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