Recombinant Human Methionine Aminopeptidase 2 Protein, CF

R&D Systems | Catalog # 3795-ZN

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human Methionine Aminopeptidase 2 Protein (3795-ZN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

P50579

Applications

Enzyme Activity
View all Methionine Aminopeptidase 2/METAP2 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Methionine Aminopeptidase 2/METAP2 protein
Ala2-Tyr478, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

54 kDa

SDS-PAGE

66 kDa, reducing conditions

Activity

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE).
The specific activity is >15 pmol/min/µg, as measured using the described conditions.

Formulation, Preparation, and Storage

3795-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Methionine Aminopeptidase 2/METAP2

The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.

References

  1. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
  2. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
  3. Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.

Alternate Names

Amp2, METAP2, MNPEP, p67eIF2

Entrez Gene IDs

10988 (Human); 56307 (Mouse); 64370 (Rat)

Gene Symbol

METAP2

UniProt

P50579

Additional Methionine Aminopeptidase 2/METAP2 Products

Product Documents for Recombinant Human Methionine Aminopeptidase 2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Methionine Aminopeptidase 2 Protein, CF

This product is covered by US Patent Nos. 5,885,820 and 5,888,796 licensed from Mediomics, LLC.

For research use only

Citations for Recombinant Human Methionine Aminopeptidase 2 Protein, CF

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Protocols

View specific protocols for Recombinant Human Methionine Aminopeptidase 2 Protein, CF (3795-ZN):

Materials
  • Assay Buffer: 50 mM HEPES, 0.1 mM CoCl2, 100 mM NaCl, pH 7.5
  • Recombinant Human Methionine Aminopeptidase 2/METAP2 (rhMETAP2) (rhMETAP2) (Catalog # 3795-ZN
  • Recombinant Human DPPIV/CD26 (rhCD26) (Catalog # 9168-SE)
  • Substrate: Met-Gly-Pro-AMC (Catalog # ES017)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhMETAP2 to 10 µg/mL in Assay Buffer.
  2. Dilute Substrate to 500 µM with 2 µg/mL rhCD26 in Assay Buffer.
  3. Load 50 µL of 10 µg/mL rhMETAP2 into a plate, and start the reaction by adding 50 µL of Substrate/rhCD26 mixture.
  4. Incubate at room temperature for 10 minutes.
  5. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes (effectively reading timepoints between 10 and 15 minutes).
  6. Calculate specific activity using the following formula:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-Amino-4-Methyl-Courmarin (AMC) (Sigma, Catalog#: A-9891).

Per Well:

  • rhMETAP2: 0.500 µg
  • rhCD26: 0.100 µg
  • Substrate: 250 µM

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