Recombinant Human MFAP4 Protein, CF

Catalog # Availability Size / Price Qty
10230-MF-050
Recombinant Human MFAP4 Protein, CF Binding Activity
2 Images
Product Details
FAQs
Reviews

Recombinant Human MFAP4 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human MFAP4 (Catalog # 10230-MF) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) nbsp; that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human MFAP4 protein
Val22-Ala255, with an N-terminal c-Myc Tag
Accession #
N-terminal Sequence
Analysis
Glu (c-Myc Tag)
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
28 kDa
SDS-PAGE
35-45 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10230-MF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human MFAP4 (Catalog # 10230-MF) is coated at 0.5 μg/mL, 100 μL/well, Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) binds with an ED50 of 0.1-0.6 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human MFAP4 (Catalog # 10230-MF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa and 70-90 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MFAP4

Microfibrillar-associated protein 4 (MFAP4) is an extracellular glycoprotein found in elastic fibers that may support elastic fiber maintenance and assembly (1). It contains fibrinogen-like domains and an RGD sequence in the N-terminus that serves as the ligand motif for the cell receptor integrin (2). MFAP4 exists as a disulfide-linked dimer that is able to form higher oligomeric structures (3). Mature human FAP4 shares 95% amino acid sequence identity with mouse and bovine MFAP4. Binding analysis revealed that MFAP4 specifically binds tropoelastin and firillin-1 and -2, as well as the elastin cross-linking amino acid desmosine, and it co‑localizes with fibrillin-1 positive fibers in vivo (1). MFAP4 contributes to the development of elastic fiber and may play a potential role in extracellular matrix (ECM) turnover during fibrogenesis (2, 4). Immunohistochemical studies showed that MFAP4 is highly abundant in hepatic stellate cells and cirrhotic ECM, but not in hepatic parenchyma or normal liver (4). MFAP4 interacts with different collectins in the lung and might fix them in the ECM during inflammation (5). Serum MFAP4 concentrations increased with progressive stages (fibrosis stage 0-4) of hepatic fibrosis in patients and has been proposed as a biomarker for hepatic fibrosis (6, 7).

References
  1. Pilecki, B. et al. (2016) J. Biol. Chem. 291:1103.
  2. Gressner, O.A. et al. (2007) J. Cell Mol. Med. 11:1031.
  3. Schlosser A. et al. (2006) Scand J Immunol. 64:104.
  4. Hunes RO. (1992) Cell. 69:11.
  5. Kasamatsu, S. et al. (2011) Sci. Rep. 1:164.
  6. Lausen, M. et al. (2017) J. Biol. Chem. 274:32234.
  7. Molleken, C. et al. (2009) Hepatology. 49:1257.
Long Name
Microfibril-associated Glycoprotein 4
Entrez Gene IDs
4239 (Human); 76293 (Mouse)
Alternate Names
MFAP4; MFAP-4; microfibril-associated glycoprotein 4; microfibrillar-associated protein 4

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human MFAP4 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human MFAP4 Protein, CF and earn rewards!

Have you used Recombinant Human MFAP4 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review