Recombinant Human MFAP4 Protein, CF Summary
Val22-Ala255, with an N-terminal c-Myc Tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human MFAP4 (Catalog # 10230-MF) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) binds with an ED50 of 0.1-0.6 µg/mL.
2 μg/lane of Recombinant Human MFAP4 (Catalog # 10230-MF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa and 70-90 kDa, respectively.
Microfibrillar-associated protein 4 (MFAP4) is an extracellular glycoprotein found in elastic fibers that may support elastic fiber maintenance and assembly (1). It contains fibrinogen-like domains and an RGD sequence in the N-terminus that serves as the ligand motif for the cell receptor integrin (2). MFAP4 exists as a disulfide-linked dimer that is able to form higher oligomeric structures (3). Mature human FAP4 shares 95% amino acid sequence identity with mouse and bovine MFAP4. Binding analysis revealed that MFAP4 specifically binds tropoelastin and firillin-1 and -2, as well as the elastin cross-linking amino acid desmosine, and it co‑localizes with fibrillin-1 positive fibers in vivo (1). MFAP4 contributes to the development of elastic fiber and may play a potential role in extracellular matrix (ECM) turnover during fibrogenesis (2, 4). Immunohistochemical studies showed that MFAP4 is highly abundant in hepatic stellate cells and cirrhotic ECM, but not in hepatic parenchyma or normal liver (4). MFAP4 interacts with different collectins in the lung and might fix them in the ECM during inflammation (5). Serum MFAP4 concentrations increased with progressive stages (fibrosis stage 0-4) of hepatic fibrosis in patients and has been proposed as a biomarker for hepatic fibrosis (6, 7).
- Pilecki, B. et al. (2016) J. Biol. Chem. 291:1103.
- Gressner, O.A. et al. (2007) J. Cell Mol. Med. 11:1031.
- Schlosser A. et al. (2006) Scand J Immunol. 64:104.
- Hunes RO. (1992) Cell. 69:11.
- Kasamatsu, S. et al. (2011) Sci. Rep. 1:164.
- Lausen, M. et al. (2017) J. Biol. Chem. 274:32234.
- Molleken, C. et al. (2009) Hepatology. 49:1257.
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