Recombinant Human mGluR2 Protein, CF

R&D Systems | Catalog # 9526-UR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human mGluR2 Protein (9526-UR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q14416
View all mGluR2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human mGluR2 protein
Gly20-Ser498, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

54 kDa

SDS-PAGE

60-75 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9526-UR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR2

Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domains, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1). The Group II receptors comprise mGLuR2 and mGLuR3, which share high sequence homology. mGLuR2 is a presynaptic receptor expressed on both neurons and glia, and its activation regulates cAMP accumulation (1). Mature human mGluR2 is 854 amino acids in length, including a 549 amino acid (aa) N-terminal ECD. Within N-terminal ECD, human mGluR2 shares 98% aa sequence identity with mouse and rat mGluR2.

References

  1. Conn P.J. and Pin J.P. (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
  2. Pin J.P. and Duvoisin R. (1995) Neuropharmacology. 34:1.
  3. Niswender C.M. and Conn P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50:295.

Long Name

Metabotropic Glutamate Receptor 2

Alternate Names

GPRC1B, GRM2, mGluR7

Entrez Gene IDs

2912 (Human); 108068 (Mouse); 24415 (Rat)

Gene Symbol

GRM2

UniProt

Q14416

Additional mGluR2 Products

Product Documents for Recombinant Human mGluR2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human mGluR2 Protein, CF

For research use only

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