Recombinant Human mGluR5 Protein, CF
Recombinant Human mGluR5 Protein, CF Summary
Ser19-Ser509, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I-III) based on sequence homology, signal transduction and pharmacological properties (1). Group I includes mGluR1 and mGluR5. This group has been shown to activate phospholipase C and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length. The 2-509 amino acids domain represents the ECD, with the apparent molecular mass approximately 64 kDa in SDS-PAGE under reducing conditions. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++ sensor receptor (4). Over aa 2-509, human mGluR5 is 98% aa identical to mouse and rat mGluR5.
- Conn PJ, et al. (1997) Annu Rev Pharmacol Toxicol 37:205.
- Pin JP, et al. (1995) Neuropharmacology. 34:1.
- Niswender CM, et al. (2010) Annu Rev Pharmacol Toxicol 50:295.
- Doré AS, et al. (2014) Nature 511:557.
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