Recombinant Human mGluR8 Protein, CF

Catalog # Availability Size / Price Qty
9534-UR-100
Recombinant Human mGluR8 Protein, CF Bioactivity not tested
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Recombinant Human mGluR8 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived human mGluR8 protein
Gln34-Ser514, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln34 inferred from enzymatic pyroglutamate treatment revealing Glu35
Structure / Form
Disulfide linked homodimer
Predicted Molecular Mass
55 kDa
SDS-PAGE
54-70 kDa, reducing conditions

Product Datasheets

9534-UR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

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Background: mGluR8

Metabotropic glutamate receptors (mGluRs) are members of the G-protein-coupled receptor (GPCR) superfamily, modulating glutamate neurotransmission in the central and peripheral nervous systems through GTP-binding proteins (1). Structurally, members of this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domains, and a cytoplasmic C-terminal domain that is variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (2). The C-terminal region is subject to alternative splicing, regulation by phosphorylation, and interacts directly with a G-protein to modulate protein-protein interactions (2, 3). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1, 2). The Group III receptors include mGLuRs 4, 6, 7 and 8 (2). Mature human mGluR8 is 875 amino acids in length, including a 550 amino acid (aa) N-terminal ECD (4). Within N-terminal ECD, human mGluR8 shares 98.4% and 98.5% aa sequence identity with mouse and rat mGluR8, respectively.

References
  1. Conn P.J. and Pin J.P. (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
  2. Niswender C.M. and Conn P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50: 295.
  3. Pin J.P. and Duvoisin R. (1995) Neuropharmacology. 34:1.
  4. Wu S. et al. (1998) Brain Res. Mol. Brain Res. 53:88.
Long Name
Metabotropic Glutamate Receptor 8
Entrez Gene IDs
2918 (Human); 14823 (Mouse); 60590 (Rat)
Alternate Names
FLJ41058; glutamate metabotropic receptor 8; glutamate receptor, metabotropic 8; GPRC1H; GPRC1HMGLUR8GLUR8; GRM8; metabotropic glutamate receptor 8; MGC126724; mGlu8; mGluR8

FAQs

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

Reconstitution Buffers

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