Recombinant Human mGluR8 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Bioassay data are not available.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived human mGluR8 protein
    Gln34-Ser514, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln34 inferred from enzymatic pyroglutamate treatment revealing Glu35
  • Structure / Form
    Disulfide linked homodimer
  • Predicted Molecular Mass
    55 kDa
  • SDS-PAGE
    54-70 kDa, reducing conditions
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9534-UR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 250 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Bioactivity not tested
R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.
Background: mGluR8
Metabotropic glutamate receptors (mGluRs) are members of the G-protein-coupled receptor (GPCR) superfamily, modulating glutamate neurotransmission in the central and peripheral nervous systems through GTP-binding proteins (1). Structurally, members of this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domains, and a cytoplasmic C-terminal domain that is variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (2). The C-terminal region is subject to alternative splicing, regulation by phosphorylation, and interacts directly with a G-protein to modulate protein-protein interactions (2, 3). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1, 2). The Group III receptors include mGLuRs 4, 6, 7 and 8 (2). Mature human mGluR8 is 875 amino acids in length, including a 550 amino acid (aa) N-terminal ECD (4). Within N-terminal ECD, human mGluR8 shares 98.4% and 98.5% aa sequence identity with mouse and rat mGluR8, respectively.
  • References:
    1. Conn P.J. and Pin J.P. (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
    2. Niswender C.M. and Conn P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50: 295.
    3. Pin J.P. and Duvoisin R. (1995) Neuropharmacology. 34:1.
    4. Wu S. et al. (1998) Brain Res. Mol. Brain Res. 53:88.
  • Long Name:
    Metabotropic Glutamate Receptor 8
  • Entrez Gene IDs:
    2918 (Human); 14823 (Mouse); 60590 (Rat)
  • Alternate Names:
    FLJ41058; glutamate metabotropic receptor 8; glutamate receptor, metabotropic 8; GPRC1H; GPRC1HMGLUR8GLUR8; GRM8; metabotropic glutamate receptor 8; MGC126724; mGlu8; mGluR8
Related Research Areas

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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC050R  
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His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC0501R
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC0501N
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His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC050U
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His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC050T
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His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC0501U
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His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC0501T
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His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC050S
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC0501G
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His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC0501S
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His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC050V
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His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC0501V
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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