Recombinant Human Monoglyceride Lipase Protein, CF

R&D Systems | Catalog # 7930-MG

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Monoglyceride Lipase Protein (7930-MG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q99685

Applications

Enzyme Activity
View all Monoglyceride Lipase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Monoglyceride Lipase protein
Pro2-Pro303, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

34 kDa

SDS-PAGE

34-37 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze 4-Nitrophenyl butyrate.
The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 4 using 7930-MG in the following applications:

Formulation, Preparation, and Storage

7930-MG
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij-35 and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Monoglyceride Lipase

Monoacylglycerol lipase, also known as MAG lipase and MGLL, is a member of the serine hydrolase superfamily with the typical serine hydrolase catalytic triad (1). It converts monoacylglycerides to free fatty acids and glycerol. An important physiological process performed by MGLL is the breakdown of the endocannabinoid 2‑arachidonoylglycerol (2-AG) by hydrolysis to provide the arachidonic acid precursor for pro‑inflammatory eicosanoid synthesis (2). MGLL knock‑out mice exhibited enhanced learning and improved performance in novel object recognition due the increased level of 2‑AG (3). In Alzheimer’s disease, inactivation of MGLL suppressed accumulation of b‑amyloid and reduced the expression of b‑site amyloid precursor protein cleaving enzyme 1 (BACE1) (4).

References

  1. Karlsson, M. et al. 1997, J. Biol. Chem. 272:27218.
  2. Nomura, D. K. et al. 2011, Science 334:809.
  3. Pan, B. et al. 2011, J. Neurosci. 31:13420.
  4. Chen, R. et al. 2012, Cell Rep. 2:1329.

Alternate Names

HUK5, MAGL, MGLL

Entrez Gene IDs

11343 (Human); 23945 (Mouse); 29254 (Rat)

Gene Symbol

MGLL

UniProt

Q99685

Additional Monoglyceride Lipase Products

Product Documents for Recombinant Human Monoglyceride Lipase Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Monoglyceride Lipase Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Citations for Recombinant Human Monoglyceride Lipase Protein, CF

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Protocols

View specific protocols for Recombinant Human Monoglyceride Lipase Protein, CF (7930-MG):

Materials
  • Assay Buffer: 50 mM Tris, pH 8.0
  • Ethanol, Absolute
  • Recombinant Human Monoglyceride Lipase (rhMGLL) (Catalog # 7930-MG)
  • Substrate:  4-Nitrophenyl butyrate (PNPB) (Sigma, Catalog # N9876), 100 mM stock in Acetone
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhMGLL to 0.8 ng/μL in Assay Buffer.
  2. Dilute Substrate to 2 mM using 40% ethanol.
  3. Load 50 μL of 0.8 ng/μL rhMGLL into the plate, and start the reaction by adding 50 μL of 2 mM Substrate.  Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of 2 mM Substrate.
  4. Read at an absorbance of 410 nm in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 4-Nitrophenol (4-NP) (Sigma, Catalog # 241326).

Per Well
  • rhMGLL: 0.04 μg
  • PNPB: 1 mM

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