Recombinant Human MSP/MST1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
352-MS-010/CF
352-MS-050/CF

With Carrier

Catalog # Availability Size / Price Qty
352-MS-010
352-MS-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
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Recombinant Human MSP/MST1 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells. 80 ng/mL of the recombinant human MSP significantly induces phosphorylation of MSP R/Ron measured by DuoSet IC human phospho-MSP R/Ron kit (Catalog # DYC1947). Measured by its binding ability in a functional ELISA. When Recombinant Human MSPR/Ron Protein (Catalog #1947-MS) is represent at 100 ng/mL (100 µg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human MSP/MST1 protein
Gln19-Gly711
Accession #
N-terminal Sequence
Analysis
No results obtained, Gln19 predicted & Val484
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
78.5 kDa (MSP alpha / beta ); 53.3 kDa ( alpha chain), 25.2 kDa ( beta chain)
SDS-PAGE
81 kDa, 62 kDa and 30 kDa, reducing conditions

Product Datasheets

352-MS (with carrier)

352-MS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

352-MS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

352-MS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSP/MST1

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 - 62 kDa alpha and a 25-35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).

References
  1. Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545.
  2. Han, S. et al. (1991) Biochemistry 30:9768.
  3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
  4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
  5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
  6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
  7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
  8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
  10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
  11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
  12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
Long Name
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Entrez Gene IDs
4485 (Human); 15235 (Mouse); 24566 (Rat)
Alternate Names
D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2

Citations for Recombinant Human MSP/MST1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. RON kinase inhibition reduces renal endothelial injury in sickle cell disease mice
    Authors: A Khaibullin, EA Adjei, N Afangbedji, A Ivanov, N Kumari, LEF Almeida, ZMN Quezado, S Nekhai, M Jerebtsova
    Haematologica, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. RON and c-Met facilitate metastasis through the ERK signaling pathway in prostate cancer cells
    Authors: B Yin, Z Liu, Y Wang, X Wang, W Liu, P Yu, X Duan, C Liu, Y Chen, Y Zhang, X Pan, H Yao, Z Liao, Z Tao
    Oncol. Rep., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Global survey of the immunomodulatory potential of common drugs
    Authors: GI Vladimer, B Snijder, N Krall, JW Bigenzahn, KVM Huber, CH Lardeau, K Sanjiv, A Ringler, UW Berglund, M Sabler, OL de la Fuen, P Knöbl, S Kubicek, T Helleday, U Jäger, G Superti-Fu
    Nat. Chem. Biol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome.
    Authors: Yang C, Kim J, Kim T, Lee P, Kim S, Lee H, Shin D, Nguyen L, Lee M, Jin H, Kim K, Lee C, Kim M, Park S, Kim J, Choi H, Jo E
    Nat Commun, 2015;6(0):6115.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Variant RONDelta160 of the RON receptor tyrosine kinase promotes the growth and invasion in vitro and in vivo in gastric cancer cell lines.
    Authors: Zhou D, Li C, Yang L
    Cancer Cell Int, 2015;15(1):9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Coordinate expression and functional profiling identify an extracellular proteolytic signaling pathway.
    Authors: Bhatt AS, Welm A, Farady CJ, Vasquez M, Wilson K, Craik CS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(14):5771-6.
    Species: Human
    Sample Types:
    Applications: Enzyme Assay Substrate
  7. Macrophage-stimulating protein differently affects human alveolar macrophages from smoker and non-smoker patients: evaluation of respiratory burst, cytokine release and NF-kappaB pathway.
    Authors: Gunella G, Bardelli C, Amoruso A
    Br. J. Pharmacol., 2006;148(4):478-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells.
    Authors: Rampino T, Collesi C, Gregorini M
    J. Am. Soc. Nephrol., 2002;13(3):649-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human MSP/MST1 Protein

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Recombinant Human MSP/MST1 Protein
By Anonymous on 09/23/2019
Application: Immunoassay Standard

We used this protein for developing an ELISA in combination with mAb (MAB352) and pAb (AF352). This combination worked well and detected human MSP in serum samples efficiently.