Recombinant Human MXRA8/DICAM Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Human MXRA8/DICAM Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 2-10 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human MXRA8/DICAM protein
His23-Gln341, with a C-terminal 6-his tag
Accession #
N-terminal Sequence
His23 & Ser34
Predicted Molecular Mass
36 kDa
38-45 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: MXRA8/DICAM

DICAM, also known as Limitrin and MXRA8, is an approximately 70 kDa transmembrane adhesion protein that is expressed on the endothelium of many tissues (1). In the vascular system, it is found on pericytes, vascular endothelial cells (EC), and the vessel-contacting feet of astrocytes (2). It is up-regulated on EC by VEGF and functions as a negative regulator of angiogenesis (3). It inhibits VEGF-induced signaling, cell migration, capillary tube formation, and in vivo angiogenesis, and promotes EC apoptosis (3). The interaction of DICAM with Integrin  alpha V beta 3 enhances cell adhesion and inhibits the differentiation of osteoclasts (1, 4). Mature human DICAM consists of a 322 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and an 80 aa cytoplasmic domain (2). Within the ECD, human DICAM shares 79% aa sequence identity with mouse and rat DICAM. Alternative splicing generates additional isoforms that lack most of the first Ig-like domain or have a substituted signal peptide.

  1. Jung, Y.K. et al. (2008) J. Cell Physiol. 216:603.
  2. Yonezawa, T. et al. (2003) Glia 44:190.
  3. Han, S.-W. et al. (2013) Cardiovasc. Res. 98:73.
  4. Jung, Y.-K. et al. (2012) J. Bone Mineral Res. 27:2024.
Long Name
Matrix-Remodelling Associated 8
Entrez Gene IDs
54587 (Human); 74761 (Mouse); 313770 (Rat)
Alternate Names
Asp3; DICAM; Limitrin; Matrix-Remodeling-Associated Protein 8; MXRA8


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human MXRA8/DICAM Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human MXRA8/DICAM Protein, CF and earn rewards!

Have you used Recombinant Human MXRA8/DICAM Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review