Recombinant Human NELL1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human NELL1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1‑4 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human NELL1 protein
Arg17-Asn810, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg17 (33%) & Phe22 (66%)
Predicted Molecular Mass
88.6 kDa & 88.1 kDa
100-130 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: NELL1

NELL1 (neural EGF‑like like protein 1) is an approximately 140 kDa modular glycoprotein that plays an important role in bone physiology (1). NELL1 contains an N‑terminal Laminin G‑like domain and three vWF‑C domains interspersed with five tandem EGF‑like domains (2). Mature human NELL1 shares 93% aa identity with mouse and rat NELL1. Alternative splicing of human NELL1 generates a short isoform that lacks the fourth EGF‑like domain. NELL1 can be retained in the cytosol where it interacts with ARP3 (apoptosis‑related protein 3) and becomes phosphorylated by PKC (3, 4). NELL1 is secreted as an approximately 400 kDa noncovalent homotrimer of heavily glycosylated subunits (5). It is expressed in bone and in select B cell lines (6, 7). NELL1 promotes the osteogenic differentiation of adipose‑derived stromal/stem cells and inhibits adipogenic differentiation (8). It does not promote osteoblastic differentiation from myoblasts, but it does enhance the activity of BMP‑2 in that function (9). NELL1 interacts directly with osteoblasts via Integrin alpha 3 beta 1 (10, 11) and promotes osteoblast differentiation and mineralization (3, 12, 13). It synergizes with BMP‑2 to increase phosphate uptake through the transporters Pit1 and Pit2 in pre‑osteoblasts (14). In vivo, NELL1 expression in the cranium is localized at sites of suture closure (6). Its over‑expression induces multiple abnormalities in cranial development including premature suture closure (craniosyntosis) and overlapping sutures (12, 13). Experimental engrafting demonstrates the ability of NELL1 to promote new bone formation and the healing of calvarial defects (13, 15).

  1. Zhang, X. et al. (2010) J. Dent. Res. 89:865.
  2. Watanabe, T.K. et al. (1996) Genomics 38:273.
  3. Zou, X. et al. (2011) FEBS Lett. 585:2410.
  4. Kuroda, S. and K. Tanizawa (1999) Biochem. Biophys. Res. Commun. 265:752.
  5. Kuroda, S. et al. (1999) Biochem. Biophys. Res. Commun. 265:79.
  6. Ting, K. et al. (1999) J. Bone Miner. Res. 14:80.
  7. Luce, M.J. and P.D. Burrows (1999) Gene 231:121.
  8. James, A.W. et al. (2012) Stem Cells Dev. 21:2170.
  9. Cowan, C.M. et al. (2007) J. Bone Miner. Res. 22:918.
  10. Hasebe, A. et al. (2012) FEBS Lett. 586:2500.
  11. Shen, J. et al. (2012) J. Cell. Biochem. 113:3620.
  12. Zhang, X. et al. (2002) J. Clin. Invest. 110:861.
  13. Aghaloo, T. et al. (2006) Am. J. Pathol. 169:903.
  14. Cowan, C.M. et al. (2012) Biochem. Biophys. Res. Commun. 422:351.
  15. Xue, J. et al. (2011) Bone 48:485.
Long Name
NEL-like 1
Entrez Gene IDs
4745 (Human); 338352 (Mouse)
Alternate Names
FLJ45906; IDH3GL; nel (chicken)-like 1; NELL1; NEL-like 1 (chicken); NEL-like protein 1; Nel-related protein 1; neural epidermal growth factor-like 1; NRP1; protein kinase C-binding protein NELL1

Citations for Recombinant Human NELL1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The roles of circRFWD2 and circINO80 during NELL-1-induced osteogenesis
    Authors: X Huang, X Cen, B Zhang, Y Liao, Z Zhao, G Zhu, Z Zhao, J Liu
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
    Authors: Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C
    Cell, 0;174(5):1158-1171.e19.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR


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