Recombinant Human NETO2 His-tag Protein, CF Summary
Ile23-Lys345, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human NETO2 His-tag Protein (Catalog # 10975-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-45 kDa.
NETO2, or Neuropilin and tolloid-like protein 2, is a type 1 transmembrane protein belonging to the CUB (for complement C1r/C1s, Ueqf, Bmp1) domain and LDLa‑containing protein family that modulates the neuronal kainate receptors (KARs) (1-3). It is mainly expressed in neural tissues but was also reported to be expressed in several non-neural tissues and its expression has been associated with various cancers such as renal, lung, colon, cervical and colorectal cancers (4-6). Mature human NETO2 is composed of a 325 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 157 aa cytoplasmic region. Within the ECD, human NETO2 shares 98% aa identity with mouse and rat NETO2. It functions as an accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3 regulating channel activities and synaptic transmission (7).
- Fedorova, M.S. et al. (2020) Front. Genet. 11:594933. doi: 10.3389/fgene.2020.594933.
- Stohr, H. et al. (2002) Gene 286:223.
- Straub, C. et al. (2011) J. Neurosci. 31:8078.
- Li, Y. et al. (2019) Cancer Manag. Res. 11:5147.
- Niu, O. et al. (2012) Genetika. 48:506.
- Xu, J. et al. (2021) Int. J. Biol. Sci. 17:259.
- Griffith T. et al. (2015) J. Physiol. 593:4815.
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