Recombinant Human Netrin-1 Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
6419-N1-025/CF
Product Details
Citations (11)
FAQs
Supplemental Products
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Recombinant Human Netrin-1 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat UNC5H2 Fc Chimera (Catalog # 1006-UN) is immobilized at 5 μg/mL, Recombinant Human Netrin-1 binds with an apparent Kd <1 nM. Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.5-2.0 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Netrin-1 protein
Human Netrin-1
(Val22-Ala604)
Accession # O95631
HPGGGSGGGSGGGS 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val22
Predicted Molecular Mass
67.5 kDa
SDS-PAGE
80-85 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6419-N1/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6419-N1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-1

Human Netrin-1 (netr: Sanskrit for “one who guides”) is a 75 kDa glycoprotein that is closely related to the laminin  gamma  domain and functions as a chemoattractive or chemorepulsive guidance cue in the central nervous system (CNS) during development (1, 2). The protein is synthesized as a 604 amino acid (aa) precursor that contains a 24 aa signal sequence and a 580 aa mature chain. Residues 46-283 constitute a laminin N-terminal domain (domain VI), while 285-453 make up three laminin-type epidermal growth factor‑like domains. There is a final domain that runs from aa 487-601 that qualifies as a Netrin-1 like domain. It is also known as domain C in the context of C. elegans. There are four potential sites for N‑linked glycosylation. Human Netrin-1 is 99% aa identical to mouse and rat Netrin-1. Netrin-1 is expressed in adult and embryonic tissues. In the adult, the protein is expressed in Schwann cells, oligodendrocytes and multiple neurons. In embryonic tissues, Netrin-1 is found in somatic mesoderm, heart, branchial pouch, and neuroepithelium. Netrin-1 is a secreted protein that, in addition to its involvement in outgrowth and migration orientation in the developing CNS, plays a significant role in the morphogenesis of endothelial cells and vascular smooth-muscle cells. It is also involved in the processes of cytoskeleton reorganization, angiogenesis, epithelial cell adhesion, and cell migration in the lungs, mammary gland, and pancreas (1, 3).

Netrin-1 effects are controlled through different transmembrane receptors (2). Four of the receptors exist in the Unc-5 (Unc=uncontrolled behaviorally) family of proteins, and these include Unc5h1, Unc5h2, Unc5h3/RCM, and Unc5h4. There are also two receptors that belong to the UNC-40 family of molecules. The first is DCC (deleted in colorectal cancer), and the second is neogenin (newly‑generated). UNC-5 receptors are noted to mediate the attraction response of axons and netrins. However, UNC-5 molecules, when co‑expressed with DCC in the presence of Netrin-1, will mediate repulsion by varying cellular calcium levels (3). The adenosine A2b receptor may also be involved in chemoattraction, either by binding directly with Netrin-1 or by serving as a co‑receptor for DCC. These receptors are known as dependence receptors because they depend on their ligand, in this case Netrin-1, for survival (4). Unbound, the receptors induce a specific death signal (4). It is the dysregulation of these receptor systems that may have important roles in tumor biology. DCC and UNC5 proteins make up a system for either initiating or inhibiting apoptosis (4), and it is now believed that Netrin-1 and its dependence receptors play a major role in tumor biology (4-6).

References
  1. Dakouane-Giudicelli, M. et al. (2010) J. Histochem. Cytochem. 58:73.
  2. Masuda, T. et al. (2008) J. of Neurosci. 28:10380.
  3. Bernet, A. and P. Mehlen (2007) Bull. Cancer 94:E12.
  4. Cirulli, V. and M. Yebra, (2007) Nat. Rev. Mol. Cell. Biol. 8:296.
  5. Dumartin, L. et al. (2010) Gastroenterology 137:1595.
  6. Mazelin, L. et al. (2004) Nature 431:80.
Entrez Gene IDs
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

Citations for Recombinant Human Netrin-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus
    Authors: CP Prieto, BS Casas, P Falcón, A Villanueva, P Lois, J Lattus, V Palma
    Int J Mol Sci, 2019;20(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Netrin-1 alleviates subarachnoid haemorrhage-induced brain injury via the PPAR?/NF-KB signalling pathway
    Authors: J Chen, Y Xuan, Y Chen, T Wu, L Chen, H Guan, S Yang, J He, D Shi, Y Wang
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis
    Authors: F Huang, M Ben Aissa, G Lévesque, M Carreau
    BMC Res Notes, 2018;11(1):662.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis
    Authors: F Huang, M Ben Aissa, G Lévesque, M Carreau
    BMC Res Notes, 2018;11(1):662.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Influence of Netrin-1 on Reinnervation of Laryngeal Muscles Following Recurrent Laryngeal Nerve Injury
    Authors: I Hernandez-, S Koss, S Sharma, MJ Pitman
    Neurosci. Lett., 2017;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  6. Intranasal administration of recombinant Netrin-1 attenuates neuronal apoptosis by activating DCC/APPL-1/AKT signaling pathway after subarachnoid hemorrhage in rats
    Authors: Z Xie, L Huang, B Enkhjargal, C Reis, W Wan, J Tang, Y Cheng, JH Zhang
    Neuropharmacology, 2017;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. Uncoupling of UNC5C with Polymerized TUBB3 in Microtubules Mediates Netrin-1 Repulsion
    Authors: Q Shao, T Yang, H Huang, F Alarmanazi, G Liu
    J. Neurosci., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation.
    Authors: Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B
    J Bone Miner Res, 2015;30(5):837-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Anchoring of protein kinase A by ERM (ezrin-radixin-moesin) proteins is required for proper netrin signaling through DCC (deleted in colorectal cancer).
    Authors: Deming P, Campbell S, Stone J, Rivard R, Mercier A, Howe A
    J Biol Chem, 2015;290(9):5783-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The Fanconi anemia group C protein interacts with uncoordinated 5A and delays apoptosis.
    Authors: Huang F, Ben Aissa M, Magron A, Huard C, Godin C, Levesque G, Carreau M
    PLoS ONE, 2014;9(3):e92811.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Netrin-1 promotes glioblastoma cell invasiveness and angiogenesis by multiple pathways including activation of RhoA, cathepsin B, and cAMP-response element-binding protein.
    Authors: Shimizu A, Nakayama H, Wang P, Konig C, Akino T, Sandlund J, Coma S, Italiano J, Mammoto A, Bielenberg D, Klagsbrun M
    J Biol Chem, 2013;288(4):2210-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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