Recombinant Human Neuregulin-3/NRG3 Fc Chimera Protein, CF

R&D Systems | Catalog # 11373-NR

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Neuregulin-3/NRG3 Fc Chimera Protein (11373-NR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P56975.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Neuregulin-3/NRG3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Neuregulin-3/NRG3 protein
Human Neuregulin-3/NRG3
(Ser93-Arg359)
Accession # P56975.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

N-terminal Sequence Analysis

Ser93

Predicted Molecular Mass

55 kDa

SDS-PAGE

90-100 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Neuregulin‑3/NRG3 Fc Chimera (Catalog # 1373-NR) binds to Recombinant Human ErbB4/Her4 Fc Chimera Protein (Catalog # 1131-ER) with a ED50 of 15.0-150 ng/mL.

Scientific Data Images for Recombinant Human Neuregulin-3/NRG3 Fc Chimera Protein, CF

Recombinant Human Neuregulin‑3/NRG3 Fc Chimera Protein SEC-MALS.

Recombinant Human Neuregulin‑3/NRG3 Fc Chimera Protein (Catalog # 11373-NR) has a molecular weight (MW) of 147.2 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human Neuregulin‑3/NRG3 Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human Neuregulin-3/NRG3 Fc Chimera (Catalog # 11373-NR) binds to Recombinant Human ErbB4/Her4 Fc Chimera Protein (1131-ER) with a ED50 of 15.0-150 ng/mL.

Recombinant Human Neuregulin‑3/NRG3 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Neuregulin‑3/NRG3 Fc Chimera Protein (Catalog # 11373-NR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-100 kDa and 180-200 kDa, respectively.

Formulation, Preparation, and Storage

11373-NR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuregulin-3/NRG3

Neuregulin-3 is a member of the neuregulin family of EGF proteins. Human NRG3, sharing 93% homology with mouse and rat NRG3, is a 78 kDa, 720 aa protein containing a 339 aa cytoplasmic domain, a 21 aa transmembrane domain, and a 360 extracellular domain with an epidermal growth factor (EGF) motif which acts as a a ligand for the ErbB4 receptor tyrosine kinase that plays pleotropic roles in neurodevelopment (1-2). Unlike many NRG1 family members but similar to sensory and motor neuron-derived factor (SMDF), NRG3 does not contain Ig-like or kringle-like domains but does contain a stretch of nonpolar aa (W66–V91). This region is thought to help translocation across the endoplasmic reticulum. NRG3 also lacks sites for N-linked glycosylation but contains many O-linked sites in a mucin-like Ser/Thr rich region. NRG3-ErbB4 was implicated in the signaling in neurodevelopment and adult brain function by promoting oligodendrocyte survival via the ErbB4/PI3K/AKT1 pathway (3). NRG3 and NRG1 can serve as potential independent biomarkers in brain lower grade glioma and glioblastoma multiforme (4). Several studies have proved that NRG1 and NRG3 play important roles in brain development, including neural plasticity (5), differentiation (6), and Schwann cell migration (7). It was also shown that NRG3 signaling acts to promote the initiation of mammary placode development and promoted the differentiation of squamous epithelia into the mammary epithelia (8).

References

  1. Zhang, D., et al. Proc. Natl. Acad. Sci. USA. 1997. 94:9562-9567.
  2. Kao, W-T. et al. PNAS. 2010. 107(35):15619-15624.
  3. Carteron, C. et al. J Cell Sci. 2006. 119:898-909.
  4. Zhao, W. et al. Front. Immunol. 2021. 12:682415.
  5. Long, LE., et al. PLoS One. 2015. 10:e0124114. doi: 10.1371/journal.pone.0124114.
  6. Zhou, Y. et al. Transl Psychiatry. 2020. 10:62. doi: 10.1038/s41398-020-0746-5.
  7. Perlin, JR. et al. Development. 2011. 138:4639–48. doi: 10.1242/dev.068072.
  8. Howard, B. et al. Genes Dev. 2005. 19:2078-90.

Alternate Names

Neuregulin3, NRG3

Entrez Gene IDs

10718 (Human); 18183 (Mouse)

Gene Symbol

NRG3

UniProt

P56975.1

Additional Neuregulin-3/NRG3 Products

Product Documents for Recombinant Human Neuregulin-3/NRG3 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Neuregulin-3/NRG3 Fc Chimera Protein, CF

For research use only

Related Research Areas

EGF Family

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