Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1131-ER-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 1.5-6 µg/mL in the presence of 10 ng/mL of Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB).
Source
Mouse myeloma cell line, NS0-derived human ErbB4/Her4 protein
Human ErbB4
(Gln26-Arg649)
Accession # Q15303
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Amino acid sequencing was blocked, suggesting it is consistent with Gln26 as the first N-terminal amino acid
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
97 kDa (monomer)
SDS-PAGE
122-132 kDa, reducing conditions

Product Datasheets

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1131-ER

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1131-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: ErbB4/Her4

ErbB4, also called Her4 (human epidermal growth factor receptor 4), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. However, ErbB4 has also been shown to heterodimerize with both ErbB1 and ErbB3. It has been suggested that the identity of the ligand may influence the dimerization partner. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has been found in ErbB4 and ErbB1 (EGFR). Several ErbB4 isoforms exist. Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme) to proteolytically cleave ErbB4 from the cell surface. These isoforms exhibit tissue-specific expression. Another isoform lacks the phosphoinositide 3-kinase activation sequence present in the ErbB4 cytoplasmic domain. Human ErbB4 consists of 1308 amino acids (aa) with a 25 aa signal sequence, a 626 aa extracellular domain, a 24 aa transmembrane region, and a 633 aa cytoplasmic domain. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer.

References
  1. Plowman, G.D. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1746. 
  2. Elenius, K. et al. (1997) J. Biol. Chem. 272:26761.
  3. Elenius, K. et al. (1999) Oncogene 18:2607.
  4. Rio, C. et al. (2000) J. Biol. Chem. 275:10379.
  5. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  6. Sundaresan, S. et al. (1998) Endocrinology 139:4756.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB4
Entrez Gene IDs
2066 (Human); 13869 (Mouse); 59323 (Rat)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; EC 2.7.10; EC 2.7.10.1; ErbB4; HER4; HER4MGC138404; p180erbB4; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Citations for Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents
    J. Mol. Biol., 2016;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Direct ELISA
  2. A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents
    Authors: Sachdev S Sidhu
    J. Mol. Biol., 2016;0(0):.
    Applications: Western Blot
  3. Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction
    Authors: Tom J Parry
    Eur. J. Pharmacol, 2016;796(0):76-89.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis.
    Authors: Leung K, Batey S, Rowlands R, Isaac S, Jones P, Drewett V, Carvalho J, Gaspar M, Weller S, Medcalf M, Wydro M, Pegram R, Mudde G, Bauer A, Moulder K, Woisetschlager M, Tuna M, Haurum J, Sun H
    Mol Ther, 2015;23(11):1722-33.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
    Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
    Neuropharmacology, 2010;59(7):640-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  6. Design of an optimized scaffold for affibody molecules.
    Authors: Feldwisch J, Tolmachev V, Lendel C, Herne N, Sjoberg A, Larsson B, Rosik D, Lindqvist E, Fant G, Hoiden-Guthenberg I, Galli J, Jonasson P, Abrahmsen L
    J. Mol. Biol., 2010;398(2):232-47.
    Applications: Dot Blot
  7. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2007;282(1):721-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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