Recombinant Human Neuronal Pentraxin 1 Protein, CF
Recombinant Human Neuronal Pentraxin 1 Protein, CF Summary
- R&D Systems NS0-derived Recombinant Human Neuronal Pentraxin 1 Protein (7707-NP)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Gln23-Asn432, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7707-NP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Neuronal Pentraxin 1
Neuronal Pentraxin (NPTX1; also called NP1) is a 47‑50 kDa secreted glycoprotein within the Pentraxin family (1, 2). NPTX1 is co‑expressed and forms heteromultimers with the related secreted protein, NPTX2/NARP, and type II transmembrane protein, NPTXR (Neuronal Pentraxin Receptor) at excitatory synapses (2‑5). Human NPTX1 is a 432 amino acid (aa) protein that includes a 22 aa signal sequence and a 410 aa secreted mature protein with one calcium‑binding Pentraxin domain (1, 2). Mature human NPTX1 shares 97% aa sequence identity with mouse, rat, bovine, porcine and canine NPTX1. NPTX1 is produced by hippocampal, cerebral and cerebellar neurons, retinal ganglia and the inner nuclear layer of the retina (1, 4‑10). NPTX1 is up‑regulated by conditions that promote neuronal apoptosis, such as amyloid‑ beta, hypoxia/ischemia, and reduction of neuronal activity by potassium deprivation, and it participates in apoptosis (7‑10). It is enriched on presynaptic axonal membranes where it forms complexes with NPTXR (3, 5). The complexes promote synaptogenesis by recruiting subunits of AMPA‑type glutamate receptors (AMPAR) (5, 11). Synaptic activity stimulates metabotropic receptors (mGluR), which then activate NPTXR cleavage and release of the complex from the membrane (4, 11). Pentraxin domains of NPTX1/2 within the complex mediate adhesion to AMPAR, while the soluble (but not transmembrane) form of NPTXR within the complex mediates AMPAR endocytosis (3, 11).
- Schlimgen, A.K. et al. (1995) Neuron 14:519.
- Omeis, I.A. et al. (1996) Genomics 36:543.
- Xu, D. et al. (2003) Neuron 39:513.
- Kirkpatrick, L.L. et al. (2000) J. Biol. Chem. 275:17786.
- Sia, G.M. et al. (2007) Neuron 55:87.
- Bjartmar, L. et al. (2006) J. Neurosci. 26:6269.
- DeGregorio-Rocasolano, N. et al. (2001) J. Biol. Chem. 276:796.
- Russell, J.C. et al. (2011) Cell Signal. 23:673.
- Abad, M.A. et al. (2006) J. Neurosci. 26:12735.
- Clayton, K.B. et al. (2012) J. Neurosci. 32:1453.
- Cho, R.W. et al. (2008) Neuron 57:858.
Citation for Recombinant Human Neuronal Pentraxin 1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Competitive inhibition of the classical complement pathway using exogenous single-chain C1q recognition proteins
Authors: H Vadászi, B Kiss, A Micsonai, G Schlosser, T Szaniszló, RÁ Kovács, BA Györffy, KA Kékesi, Y Goto, B Uzonyi, K Liliom, J Kardos
Oncogene, 2022-06-09;0(0):102113.
Species: Human
Sample Types: Recombinant Proteins
Applications: ELISA Capture
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