Recombinant Human Neurturin Protein

Carrier Free

Catalog # Availability Size / Price Qty
1297-NE-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1297-NE-025

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Recombinant Human Neurturin Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 20-80 ng/mL in the presence of Recombinant Human GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 613‑FR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 613-FR) at 1 μg/mL can bind Recombinant Human Neurturin with an apparent KD <3.0 nM.
Source
E. coli-derived human Neurturin protein
Ala96-Val197, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
11.8 kDa (monomer)

Product Datasheets

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1297-NE (with carrier)

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1297-NE/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1297-NE

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1297-NE/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Neurturin

Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similarly to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Mature human Neurturin shares approximately 92% amino acid sequence identity with mouse Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2-5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentrations (5‑8). Neurturin had been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems and as well as non-neuronal systems (9).

References
  1. Lin, L-F.H. et al. (1993) Science 260:1130.
  2. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  3. Milbrandt, J. et al. (1998) Neuron 20:245.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
  9. Airaksinen. M. and M. Saarma (2002) Nature Review Neuroscience 3:383.
Entrez Gene IDs
4902 (Human); 18188 (Mouse)
Alternate Names
Neurturin; NRTN; NTN

Citations for Recombinant Human Neurturin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency
    Authors: B Sun, X Yang, F Hou, X Yu, Q Wang, HS Oh, P Raja, JM Pesola, EAH Vanni, S McCarron, J Morris-Lov, AHM Ng, GM Church, DM Knipe, DM Coen, D Pan
    Nature Microbiology, 2021-02-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Long-term post-mortem studies following neurturin gene therapy in patients with advanced Parkinson's disease.
    Authors: Chu Y, Bartus R, Manfredsson F, Olanow C, Kordower J
    Brain, 2020-03-01;143(3):960-975.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Adsorption
  3. Unmasking a new prognostic marker and therapeutic target from the GDNF-RET/PIT1/p14ARF/p53 pathway in acromegaly
    Authors: M Chenlo, IA Rodriguez-, R Serramito, AR Garcia-Ren, R Villar-Tai, E Fernandez-, S Perez-Rome, M Suarez-Far, A Garcia-All, JM Cabezas-Ag, J Rodriguez-, PV Lear, RM Alvarez-Sa, C Alvarez-Es, I Bernabeu, CV Alvarez
    EBioMedicine, 2019-04-08;0(0):.
    Species: Human
    Sample Types: NFPA Cells
    Applications: Cell Culture
  4. Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction
    Authors: JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman
    Mol Ther Methods Clin Dev, 2018-02-23;9(0):172-180.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Human, Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  6. Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
    Authors: Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS
    Neuropharmacology, 2010-02-11;58(7):1114-21.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008-06-09;76(8):3530-8.
    Species: Bacteria
    Sample Types: Trypanosoma
    Applications: ELISA (Capture)
  8. Regulation of neuronal pituitary adenylate cyclase-activating polypeptide expression during culture of guinea-pig cardiac ganglia.
    Authors: Girard BM, Young BA, Buttolph TR, White SL, Parsons RL
    Neuroscience, 2007-03-23;146(2):584-93.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons.
    Authors: Girard BM, Young BA, Buttolph TR, Locknar SA, White SL, Parsons RL
    Neuroscience, 2006-03-03;139(4):1329-41.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: Bioassay

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