Recombinant Human Ninjurin-1 Protein, CF Summary
with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.
Ninjurin-1 (nerve injury-induced protein 1) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins (1, 2). It is expressed by Schwann cells, neurons and hepatocytes and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37 (2). Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. Human Ninjurin-1 shares only 50% aa sequence identity with human Ninjurin-2. Expression of NINJ‑1 increased under inflammation conditions, and itself can also exert proinflammatory effects such as stimulating leukocyte migration, and activating TLR4 signaling pathway (3). Inhibiting hemophilic interaction of NINJ1 in circulating tumor cells greatly inhibit the mobility of the cancer cells (4).
- Araki, T. and Milbrandt J. (1996) Neuron 17:353.
- Araki, T. and Milbrandt J. (1997) J. Biol Chem 272:21373.
- Jennewein, C. et. al. (2015) Am J Respir Cell Mol Biol 53:656.
- Park, J. et. al. (2017) Anticancer Res 37:1687.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Ninjurin-1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Ninjurin-1 Protein, CF and earn rewards!
Have you used Recombinant Human Ninjurin-1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image