Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF

R&D Systems | Catalog # 10371-NK

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human NKp65/KLRF2 Fc Chimera Protein (10371-NK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

D3W0D1.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all NKp65/KLRF2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human NKp65/KLRF2 protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human NKp65/KLRF2
(Asp52-Val207)
Accession # D3W0D1.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met Asp - Pro100

Predicted Molecular Mass

45 kDa

SDS-PAGE

50-65 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) is immobilized 0.1 μg the concentration of Recombinant Human CLEC-2A (Catalog # 8435-CL) the optimal binding response is approximately 0.5-3 ng/mL.

Scientific Data Images for Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF

Recombinant Human NKp65/KLRF2 Fc Chimera Protein Binding Activity

Recombinant Human NKp65/KLRF2 Fc Chimera Protein Binding Activity

When Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) is immobilized at 0.1 µg/mL, 100 µL/well, the concentration for Recombinant Human CLEC-2A (Catalog # 8435-CL) that produces 50% of the optimal binding response is approximately 0.5-3 ng/mL.
Recombinant Human NKp65/KLRF2 Fc Chimera Protein SDS-PAGE

Recombinant Human NKp65/KLRF2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-65 kDa and 100-130 kDa, respectively.

Formulation, Preparation, and Storage

10371-NK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKp65/KLRF2

Killer cell lectin-like receptor subfamily F member 2, also known as KLRF2 and NKp65, is a C-type lectin-like receptor and a member of the NKRP1 (NK receptor protein) subfamily (1-3). KLRF2, along with the related KLRF1, is an activating receptor for the C-type-lectin-like-2 (CLEC2) family of ligands (1-3). KLRF2 is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic domain, a single transmembrane region and an extracellular domain (ECD) consisting of 26-residue stalk and 130-residue C-type lectin-like domain (CTLD) (1, 3). Unlike other members of the NKRP1 family such as KLRF1, KLRF2 is a non-disulfide-linked homodimer (1, 2). While sharing subfamily-specific traits classifying them as members of the NKRP1 subfamily, there does not appear to be a direct homolog of KLRF2 in mouse (2). KLRF2 is minimally expressed on peripheral blood NK cells and its ligand, Keratinocyte-associated C-type lectin (KACL), is restricted to human keratinocytes (1-3). This expression of KACL indicates potential implications in skin-affecting diseases such as psoriasis and wound healing (1). The signaling pathway of KLRF2 is distinct from most NK receptors because it does not utilize immunoreceptor tyrosine-based activating motif (ITAM)-containing signaling adaptors, but rather uses an activating signaling motif with only one tyrosine module referred to as hemITAM (1, 3).

References

  1. Spreu, J. et al. (2010) Proc Natl Acad Sci USA 107:5100.
  2. Bartel, Y. et al. (2013) Front Immunol 4:362.
  3. Li, Y. et al. (2013) Proc Natl Acad Sci USA 110:11505.

Alternate Names

KLRF 2, KLRF2

Entrez Gene IDs

100431172 (Human)

Gene Symbol

KLRF2

UniProt

D3W0D1.1

Additional NKp65/KLRF2 Products

Product Documents for Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF

For research use only

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