Recombinant Human Notum Protein, CF

R&D Systems | Catalog # 9118-NM

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Notum Protein (9118-NM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q6P988

Applications

Bioactivity
View all Notum Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Notum protein
Ser81-Thr451 (Cys330Ser) with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser81

Predicted Molecular Mass

43 kDa

SDS-PAGE

38-45 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells.
The ED50 for this effect is 0.1-0.6 μg/mL in the presence of 0.5 μg/mL of Recombinant Human Wnt-3a (Catalog # 5036-WN).

Reviewed Applications

Read 1 review rated 5 using 9118-NM in the following applications:

Scientific Data Images for Recombinant Human Notum Protein, CF

Recombinant Human Notum Protein Bioactivity

Recombinant Human Notum Protein Bioactivity

Recombinant Human Wnt-3a shows a dose responsive increase in Wnt reporter activity in HEK293 cells (green line). Recombinant Human Notum (Catalog # 9118-NM) inhibits a constant dose of 0.5 μg/mL Recombinant Human Wnt-3a (Catalog # 5036-WN). The ED50 is 0.1-0.6 µg/mL (orange line).

Formulation, Preparation, and Storage

9118-NM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Notum

Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids
81-451, human Notum shares 92% aa sequence identity with mouse and rat Notum.

References

  1. Zhang, X. et al. (2015) Dev. Cell 32:719.
  2. Kakugawa, S. et al. (2015) Nature 519:187.
  3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
  4. Traister, A. et al. (2008) Biochem. J. 410:503.
  5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
  6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
  7. De Robertis, M. et al. (2015) Oncotarget 6:41237.

Alternate Names

EC 3.-, notum pectinacetylesterase homolog (Drosophila), protein notum homolog

Entrez Gene IDs

147111 (Human); 77583 (Mouse); 303743 (Rat)

Gene Symbol

NOTUM

UniProt

Q6P988

Additional Notum Products

Product Documents for Recombinant Human Notum Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Notum Protein, CF

For research use only

Related Research Areas

Wnt Inhibitors

Citations for Recombinant Human Notum Protein, CF

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