>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1-0.6 μg/mL in the presence of 0.5 μg/mL of
Human Wnt-3a (Catalog # 5036-WN).
Chinese Hamster Ovary cell line, CHO-derived human Notum protein Ser81-Thr451 (Cys330Ser) with a C-terminal 6-His tag
Recombinant Human Wnt-3a shows a dose responsive increase in Wnt reporter activity in HEK293 cells (green line). Recombinant Human Notum (Catalog # 9118-NM) inhibits a constant dose of 0.5 μg/mL Recombinant Human Wnt-3a (Catalog # 5036-WN). The ED50 is 0.1-0.6 µg/mL (orange line).
Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to
the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions
as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl
moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the
Frizzled-8 receptor (1, 2). It also induces the release of cell surface
GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum
regulates head regeneration in planaria (3), head formation and neural
induction in Xenopus (1), and long range activity of hedgehog proteins in
Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and
colorectal cancers (6, 7). Within amino acids 81-451, human Notum shares 92% aa
sequence identity with mouse and rat Notum.
Zhang, X. et al. (2015) Dev. Cell 32:719.
Kakugawa, S. et al. (2015) Nature 519:187.
Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
Traister, A. et al. (2008) Biochem. J. 410:503.
Ayers, K.L. et al. (2010) Dev. Cell 18:605.
Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
De Robertis, M. et al. (2015) Oncotarget 6:41237.
Entrez Gene IDs:
147111 (Human); 77583 (Mouse); 303743 (Rat)
EC 3.-; notum pectinacetylesterase homolog (Drosophila); Notum; protein notum homolog
Have you used Recombinant Human Notum Protein, CF?
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