Recombinant Human Wnt-3a Protein Summary
Product Specifications
Optimal concentrations should be determined by each laboratory for each application.
Ser19-Lys352
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5036-WN
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
5036-WN/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS. |
Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Image

Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The ED50is 1.7-fold better with more than twice the maximum response compared to the top competitor's Wnt-3a.
Reconstitution Calculator
Background: Wnt-3a
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).
- Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
- MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
- Korinek, V. et al. (1997) Science 275:1784.
- Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
- Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
- Takada, R. et al. (2006) Dev. Cell 11:791.
- Komekado, H. (2007) Genes Cells 12:521.
- Dunty Jr. W. C. et al. (2008) Development 135:85.
- Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
- Lee, S. M. et al. (2000) Development 127:457.
- Takada, S. et al. (1994) Genes Dev. 8:174.
- Willert, K. et al. (2003) Nature 423:6938.
- Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
- Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.
Citations for Recombinant Human Wnt-3a Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Familial hypercholesterolemia class II low density lipoprotein-receptor response to statin treatment
Authors: L Omer, L Hindi, G Militello, KB Stivers, KC Tien, NL Boyd
Dis Model Mech, 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
SULF1 suppresses Wnt3A-driven growth of bone metastatic prostate cancer in perlecan-modified 3D cancer-stroma-macrophage triculture models
Authors: FH Brasil da, MS Lewis, A Truong, DD Carson, MC Farach-Car
PLoS ONE, 2020;15(5):e0230354.
Species: Human
Sample Types:
Applications: Cell Culture -
FASN-Dependent Lipid Metabolism Links Neurogenic Stem/Progenitor Cell Activity to Learning and Memory Deficits
Authors: M Bowers, T Liang, D Gonzalez-B, S Zocher, BN Jaeger, WJ Kovacs, C Röhrl, KML Cramb, J Winterer, M Kruse, S Dimitrieva, RW Overall, T Wegleiter, H Najmabadi, CF Semenkovic, G Kempermann, C Földy, S Jessberger
Cell Stem Cell, 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells
Authors: E Flores-Her, DM Velázquez, MC Castañeda-, G Fuentes-Ga, G Fonseca-Ca, JK Yamamoto-F, MT Romero-Avi, JA García-Sái, M Robles-Flo
Cell. Signal., 2020;72(0):109636.
Species: Human
Sample Types: Whole Cell
Applications: Bioassay -
Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
Sci Rep, 2020;10(1):5989.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells
Authors: F He, N Li, HB Huang, JB Wang, XF Yang, HD Wang, W Huang, FR Li
Stem Cell Res Ther, 2020;11(1):163.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription
Authors: Q Li, Y Sun, GK Jarugumill, S Liu, K Dang, JL Cotton, J Xiol, PY Chan, M DeRan, L Ma, R Li, LJ Zhu, JH Li, AB Leiter, YT Ip, FD Camargo, X Luo, RL Johnson, X Wu, J Mao
Cell Stem Cell, 2020;26(5):675-692.e8.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein
Authors: X Shi, W Lin, X Gao, W Xie, JA Golden, T Tao
Biosci. Rep., 2020;40(7):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expression of MALAT1 Promotes Trastuzumab Resistance in HER2 Overexpressing Breast Cancers
Authors: Y Wu, M Sarkissyan, O Ogah, J Kim, JV Vadgama
Cancers (Basel), 2020;12(7):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency
Authors: W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita
J. Clin. Invest., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts
Authors: R Castro-Viñ, C Sanjurjo-R, M Piñeiro-Ra, T Hermida-Gó, S Rodríguez-, N Oreiro, J de Toro, I Fuentes, FJ Blanco, S Díaz-Prado
Sci Rep, 2020;10(1):4272.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation
Authors: A Fleischer, S Vallejo-Dí, JM Martín-Fer, A Sánchez-Gi, M Castresana, A Del Pozo, A Esquisabel, S Ávila, JL Castrillo, E Gaínza, JL Pedraz, M Viñas, D Bachiller
Mol Ther Methods Clin Dev, 2020;17(0):858-870.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
The Transcription Factor-microRNA Regulatory Network during hESC-chondrogenesis
Authors: R Griffiths, S Woods, A Cheng, P Wang, S Griffiths-, M Ronshaugen, SJ Kimber
Sci Rep, 2020;10(1):4744.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Structural insight into small molecule action on Frizzleds
Authors: P Kozielewic, A Turku, CF Bowin, J Petersen, J Valnohova, MCA Cañizal, Y Ono, A Inoue, C Hoffmann, G Schulte
Nat Commun, 2020;11(1):414.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Induced pluripotent stem cell-derived tenocyte-like cells promote the regeneration of injured tendons in mice
Authors: S Komura, T Satake, A Goto, H Aoki, H Shibata, K Ito, A Hirakawa, Y Yamada, H Akiyama
Sci Rep, 2020;10(1):3992.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dishevelled 1-Regulated Superpotent Cancer Stem Cells Mediate Wnt Heterogeneity and Tumor Progression in Hepatocellular Carcinoma
Authors: WY Liao, CC Hsu, TS Chan, CJ Yen, WY Chen, HW Pan, KK Tsai
Stem Cell Reports, 2020;14(3):462-477.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells
Authors: V Uribe-Etxe, P García-Gal, M Pérez-Garr, M Casado-And, I Irastorza, F Unda, G Ibarretxe, N Subirán
Cells, 2020;9(3):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
A hepatocyte differentiation model reveals two subtypes of liver cancer with different oncofetal properties and therapeutic targets
Authors: M Liu, Q Yan, Y Sun, Y Nam, L Hu, JH Loong, Q Ouyang, Y Zhang, HL Li, FE Kong, L Li, Y Li, MM Li, W Cheng, LX Jiang, S Fang, XD Yang, JQ Mo, YF Gong, YQ Tang, Y Li, YF Yuan, NF Ma, G Lin, S Ma, JG Wang, XY Guan
Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Norrin mediates tumor-promoting and -suppressive effects in glioblastoma via Notch and WNT
Authors: A El-Sehemy, HJ Selvadurai, A Ortin-Mart, NT Pokrajac, Y Mamatjan, N Tachibana, KJ Rowland, L Lee, NI Park, KD Aldape, P Dirks, VA Wallace
J. Clin. Invest., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells
Authors: KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son
J Clin Med, 2019;8(7):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett's associated esophageal adenocarcinoma
Authors: O Lyros, AK Lamprecht, L Nie, R Thieme, K Götzel, M Gasparri, G Haasler, P Rafiee, R Shaker, I Gockel
Am J Cancer Res, 2019;9(2):330-346.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
In-depth characterization of the Wnt-signaling/?-catenin pathway in an in vitro model of Barrett's sequence
Authors: K Götzel, O Chemnitzer, L Maurer, A Dietrich, U Eichfeld, O Lyros, Y Moulla, S Niebisch, M Mehdorn, B Jansen-Win, M Vieth, A Hoffmeiste, I Gockel, R Thieme
BMC Gastroenterol, 2019;19(1):38.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Point mutations in the PDX1 transactivation domain impair human ?-cell development and function
Authors: X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert
Mol Metab, 2019;0(0):.
Species: Human
Sample Types: Whole Cells
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Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure
Authors: V Lungova, X Chen, Z Wang, C Kendziorsk, SL Thibeault
Nat Commun, 2019;10(1):4161.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Induction of Expandable Tissue-Specific Progenitor Cells from Human Pancreatic Tissue through Transient Expression of Defined Factors
Authors: H Noguchi, C Miyagi-Shi, Y Nakashima, T Kinjo, N Kobayashi, I Saitoh, M Watanabe, AMJ Shapiro, T Kin
Mol Ther Methods Clin Dev, 2019;13(0):243-252.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Gfi1b regulates the level of Wnt/?-catenin signaling in hematopoietic stem cells and megakaryocytes
Authors: P Shooshtari, A Helness, C Vadnais, N Brouwer, H Beauchemin, R Chen, H Bagci, FJT Staal, JF Coté, T Möröy
Nat Commun, 2019;10(1):1270.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Excess growth hormone suppresses DNA damage repair in epithelial cells
Authors: V Chesnokova, S Zonis, R Barrett, H Kameda, K Wawrowsky, A Ben-Shlomo, M Yamamoto, J Gleeson, C Bresee, V Gorbunova, S Melmed
JCI Insight, 2019;4(3):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
Cell Rep, 2019;26(10):2566-2579.e10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos
Authors: L Liu, L Leng, C Liu, C Lu, Y Yuan, L Wu, F Gong, S Zhang, X Wei, M Wang, L Zhao, L Hu, J Wang, H Yang, S Zhu, F Chen, G Lu, Z Shang, G Lin
Nat Commun, 2019;10(1):364.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Derivation of Brain Capillary-like Endothelial Cells from Human Pluripotent Stem Cell-Derived Endothelial Progenitor Cells
Authors: C Praça, SC Rosa, E Sevin, R Cecchelli, MP Dehouck, LS Ferreira
Stem Cell Reports, 2019;13(4):599-611.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling
Authors: S Akbari, GG Sevinç, N Ersoy, O Basak, K Kaplan, K Sevinç, E Ozel, B Sengun, E Enustun, B Ozcimen, A Bagriyanik, N Arslan, TT Önder, E Erdal
Stem Cell Reports, 2019;13(4):627-641.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
In vitro metabolic zonation through oxygen gradient on a chip
Authors: F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore
Sci Rep, 2019;9(1):13557.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism
Authors: RR Stine, AP Sakers, T TeSlaa, M Kissig, ZE Stine, CW Kwon, L Cheng, HW Lim, KH Kaestner, JD Rabinowitz, P Seale
Cell Stem Cell, 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Microenvironmental Factors Drive Tenascin C and Src Cooperation to Promote Invadopodia Formation in Ewing Sarcoma
Authors: AG Hawkins, CM Julian, S Konzen, S Treichel, ER Lawlor, KM Bailey
Neoplasia, 2019;21(10):1063-1072.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Proton pump inhibitors attenuate myofibroblast formation associated with thyroid eye disease through the aryl hydrocarbon receptor
Authors: CL Hammond, E Roztocil, RP Phipps, SE Feldon, CF Woeller
PLoS ONE, 2019;14(9):e0222779.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
COL4A2 in the tissue-specific extracellular matrix plays important role on osteogenic differentiation of periodontal ligament stem cells
Authors: Y Wen, H Yang, J Wu, A Wang, X Chen, S Hu, Y Zhang, D Bai, Z Jin
Theranostics, 2019;9(15):4265-4286.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
USP9X Deubiquitylates DVL2 to Regulate WNT Pathway Specification
Authors: CP Nielsen, KK Jernigan, NL Diggins, DJ Webb, JA MacGurn
Cell Rep, 2019;28(4):1074-1089.e5.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Enhanced chondrogenesis from human embryonic stem cells
Authors: T Wang, P Nimkingrat, CA Smith, A Cheng, TE Hardingham, SJ Kimber
Stem Cell Res, 2019;39(0):101497.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells
Authors: KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son
J Clin Med, 2019;8(7):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite
Authors: K Abe, A Shimada, S Tayama, H Nishikawa, T Kaneko, S Tsuda, A Karaiwa, T Matsui, T Ishitani, H Takeda
Cell Rep, 2019;27(3):928-939.e4.
Species: Fish - Oryzias latipes (Medaka/Rice Fish)
Sample Types: In Vivo
Applications: In Vivo -
WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis
Authors: K Kaiser, D Gyllborg, J Procházka, A Salašová, P Kompaníkov, FL Molina, R Laguna-Goy, T Radaszkiew, J Harnoš, M Procházkov, D Pot?šil, RA Barker, ÁG Casado, Z Zdráhal, R Sedlá?ek, E Arenas, JC Villaescus, V Bryja
Nat Commun, 2019;10(1):1498.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Controlled modelling of human epiblast and amnion development using stem cells
Authors: Y Zheng, X Xue, Y Shao, S Wang, SN Esfahani, Z Li, JM Muncie, JN Lakins, VM Weaver, DL Gumucio, J Fu
Nature, 2019;573(7774):421-425.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling
Authors: R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke
Nat Commun, 2019;10(1):5016.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells
Authors: T Kijima, H Nakagawa, M Shimonoson, PM Chandramou, T Hara, V Sahu, Y Kasagi, O Kikuchi, K Tanaka, V Giroux, AB Muir, KA Whelan, S Ohashi, S Naganuma, AJ Klein-Szan, Y Shinden, K Sasaki, I Omoto, Y Kita, M Muto, AJ Bass, JA Diehl, GG Ginsberg, Y Doki, M Mori, Y Uchikado, T Arigami, NG Avadhani, D Basu, AK Rustgi, S Natsugoe
Cell Mol Gastroenterol Hepatol, 2019;7(1):73-91.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
ZNF326 promotes malignant phenotype of glioma by up-regulating HDAC7 expression and activating Wnt pathway
Authors: X Yu, M Wang, J Wu, Q Han, X Zhang
J. Exp. Clin. Cancer Res., 2019;38(1):40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner
Authors: AP Singh, YH Hung, MT Shanahan, M Kanke, A Bonfini, MK Dame, M Biraud, BCE Peck, OO Oyesola, JM Freund, RL Cubitt, EG Curry, LM Gonzalez, GA Bewick, ED Tait-Wojno, NA Kurpios, S Ding, JR Spence, CM Dekaney, N Buchon, P Sethupathy
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Porcine
Sample Types: Whole Tissue
Applications: Tissue Culture -
Immunomodulatory effects of interferon-gamma on human fetal cardiac mesenchymal stromal cells
Authors: KH Grinnemo, M Löfling, L Nathanson, R Baumgartne, DFJ Ketelhuth, V Beljanski, LC Davies, C Österholm
Stem Cell Res Ther, 2019;10(1):371.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of ERK1/2 in cancer-associated pancreatic stellate cells suppresses cancer-stromal interaction and metastasis
Authors: Z Yan, K Ohuchida, S Fei, B Zheng, W Guan, H Feng, S Kibe, Y Ando, K Koikawa, T Abe, C Iwamoto, K Shindo, T Moriyama, K Nakata, Y Miyasaka, T Ohtsuka, K Mizumoto, M Hashizume, M Nakamura
J. Exp. Clin. Cancer Res., 2019;38(1):221.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Wnt Glycation Inhibits Canonical Signaling
Authors: Z Ye, S Mittag, M Schmidt, A Simm, R Horstkorte, O Huber
Cells, 2019;8(11):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips
Authors: MJ Workman, JP Gleeson, EJ Troisi, HQ Estrada, SJ Kerns, CD Hinojosa, GA Hamilton, SR Targan, CN Svendsen, RJ Barrett
Cell Mol Gastroenterol Hepatol, 2018;5(4):669-677.e2.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells
Authors: S Yamada, Y Kubo, D Yamazaki, Y Sekino, Y Nomura, S Yoshida, Y Kanda
Sci Rep, 2018;8(1):12155.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Genome-wide analysis of PDX1 target genes in human pancreatic progenitors
Authors: X Wang, M Sterr, I Burtscher, S Chen, A Hieronimus, F Machicao, H Staiger, HU Häring, G Lederer, T Meitinger, FM Cernilogar, G Schotta, M Irmler, J Beckers, M Hrab? de A, M Ray, CVE Wright, M Bakhti, H Lickert
Mol Metab, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling
Authors: S Kokabu, V Rosen
FEBS Open Bio, 2018;8(2):168-176.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation
Authors: KE Worley, AS Chin, LQ Wan
Stem Cells Int, 2018;2018(0):1848605.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IKK? is a ?-catenin kinase that regulates mesenchymal stem cell differentiation
Authors: Y Sui, Z Liu, SH Park, SE Thatcher, B Zhu, JP Fernandez, H Molina, PA Kern, C Zhou
JCI Insight, 2018;3(2):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A20 regulates canonical wnt-signaling through an interaction with RIPK4
Authors: BN Nakamura, A Glazier, MG Kattah, B Duong, Y Jia, D Campo, L Shao
PLoS ONE, 2018;13(5):e0195893.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tissue transglutaminase regulates interactions between ovarian cancer stem cells and the tumor niche
Authors: S Condello, LE Sima, C Ivan, H Cardenas, GE Schiltz, RK Mishra, D Matei
Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Natural molecules induce and synergize to boost expression of the human antimicrobial peptide ?-defensin-3
Authors: E Sechet, E Telford, C Bonamy, PJ Sansonetti, B Sperandio
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
An essential role for Wnt/?-catenin signaling in mediating hypertensive heart disease
Authors: Y Zhao, C Wang, C Wang, X Hong, J Miao, Y Liao, L Zhou, Y Liu
Sci Rep, 2018;8(1):8996.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
The Wnt-?-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells
Authors: H He, F Du, Y He, Z Wei, C Meng, Y Xu, H Zhou, N Wang, XG Luo, W Ma, TC Zhang
Oncotarget, 2018;9(20):15239-15251.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips.
Authors: Michael J Workman, John P Gleeson, Elissa J Troisi, Hannah Q Estrada, S Jordan Kerns, Christopher D Hinojosa, Geraldine A Hamilton, Stephan R Targan, Clive N Svendsen, Robert J Barrett
Cellular and Molecular Gastroenterology and Hepatology, 2018;0(0):2352-345X.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Transcriptome 3'end organization by PCF11 links alternative polyadenylation to formation and neuronal differentiation of neuroblastoma
Authors: A Ogorodniko, M Levin, S Tattikota, S Tokalov, M Hoque, D Scherzinge, F Marini, A Poetsch, H Binder, S Macher-Göp, HC Probst, B Tian, M Schaefer, KJ Lackner, F Westermann, S Danckwardt
Nat Commun, 2018;9(1):5331.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors
Authors: H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko
Stem Cell Res Ther, 2018;9(1):277.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family
Authors: J Li, C Narayanan, J Bian, D Sambo, T Brickler, W Zhang, S Chetty
PLoS ONE, 2018;13(12):e0208110.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expression of the human antimicrobial peptide ?-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells
Authors: C Bonamy, E Sechet, A Amiot, A Alam, M Mourez, L Fraisse, PJ Sansonetti, B Sperandio
Sci Rep, 2018;8(1):18043.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling
Authors: B Cha, X Geng, MR Mahamud, JY Zhang, L Chen, W Kim, EH Jho, Y Kim, D Choi, JB Dixon, H Chen, YK Hong, L Olson, TH Kim, BJ Merrill, MJ Davis, RS Srinivasan
Cell Rep, 2018;25(3):571-584.e5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages
Authors: J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao
Cell Transplant, 2018;0(0):9636897187803.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype
Authors: M Amin, Y Kushida, S Wakao, M Kitada, K Tatsumi, M Dezawa
Cell Transplant, 2018;27(2):285-298.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells
Authors: BA Brown, GM Connolly, CEJ Mill, H Williams, GD Angelini, JL Johnson, SJ George
Aging Cell, 2018;0(0):e12844.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
EPH receptor A2 governs a feedback loop that activates Wnt/?-catenin signaling in gastric cancer
Authors: Q Peng, L Chen, W Wu, J Wang, X Zheng, Z Chen, Q Jiang, J Han, L Wei, L Wang, J Huang, J Ma
Cell Death Dis, 2018;9(12):1146.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
GLIS3 Transcriptionally Activates WNT Genes to Promote Differentiation of Human Embryonic Stem Cells into Posterior Neural Progenitors
Authors: K Jeon, D Kumar, AE Conway, K Park, R Jothi, AM Jetten
Stem Cells, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoassay Development -
Reliance upon ancestral mutations is maintained in colorectal cancers that heterogeneously evolve during targeted therapies
Authors: M Russo, S Lamba, A Lorenzato, A Sogari, G Corti, G Rospo, B Mussolin, M Montone, L Lazzari, S Arena, D Oddo, M Linnebache, A Sartore-Bi, F Pietranton, S Siena, F Di Nicolan, A Bardelli
Nat Commun, 2018;9(1):2287.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation
Authors: R Diacou, Y Zhao, D Zheng, A Cvekl, W Liu
Cell Rep, 2018;25(9):2510-2523.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system
Authors: D Garnier, R Li, F Delbos, A Fourrier, C Collet, C Guguen-Gui, C Chesné, TH Nguyen
Sci Rep, 2018;8(1):8222.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
The Ewing sarcoma secretome and its response to activation of Wnt/beta-catenin signaling
Authors: AG Hawkins, V Basrur, F da Veiga L, E Pedersen, C Sperring, AI Nesvizhski, ER Lawlor
Mol. Cell Proteomics, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
-
Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids
Authors: E Magro-Lope, C Palmer, J Manso, I Liste, A Zambrano
Stem Cell Res Ther, 2018;9(1):186.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma
Authors: M Szemes, A Greenhough, Z Melegh, S Malik, A Yuksel, D Catchpoole, K Gallacher, M Kollareddy, JH Park, K Malik
Neoplasia, 2018;20(4):335-350.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Ubiquitylation and degradation of adenomatous polyposis coli by MKRN1 enhances Wnt/?-catenin signaling
Authors: HK Lee, EW Lee, J Seo, M Jeong, SH Lee, SY Kim, EH Jho, CH Choi, JY Chung, J Song
Oncogene, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In�Vitro
Authors: A Sarkar, A Mei, ACM Paquola, S Stern, C Bardy, JR Klug, S Kim, N Neshat, HJ Kim, M Ku, MN Shokhirev, DH Adamowicz, MC Marchetto, R Jappelli, JA Erwin, K Padmanabha, M Shtrahman, X Jin, FH Gage
Cell Stem Cell, 2018;22(5):684-697.e9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
LGR5 and BMI1 Increase Pig Intestinal Epithelial Cell Proliferation by Stimulating WNT/?-Catenin Signaling
Authors: XG Li, Z Wang, RQ Chen, HL Fu, CQ Gao, HC Yan, GX Xing, XQ Wang
Int J Mol Sci, 2018;19(4):.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
PBX3 is part of an EMT regulatory network and indicates poor outcome in colorectal cancer
Authors: S Lamprecht, M Kaller, EM Schmidt, C Blaj, TS Schiergens, J Engel, A Jung, H Hermeking, TGP Grünewald, T Kirchner, D Horst
Clin. Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Stimulation -
Cholesterol Esterification Inhibition Suppresses Prostate Cancer Metastasis by Impairing the Wnt/?-catenin Pathway
Authors: HJ Lee, J Li, RE Vickman, J Li, R Liu, A Durkes, BD Elzey, S Yue, X Liu, TL Ratliff, JX Cheng
Mol. Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TGF? activation primes canonical Wnt signaling through the downregulation of AXIN2
Authors: J Gillespie, RL Ross, C Corinaldes, F Esteves, E Derrett-Sm, MF McDermott, GM Doody, CP Denton, P Emery, F Del Galdo
Arthritis & rheumatology (Hoboken, N.J.), 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
A simple and scalable hydrogel-based system for culturing protein-producing cells
Authors: Q Li, Q Wang, O Wang, K Shao, H Lin, Y Lei
PLoS ONE, 2018;13(1):e0190364.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells
Authors: J Jang, Y Jung, S Chae, SI Chung, SM Kim, Y Yoon
Biochem. Biophys. Res. Commun, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Ubiquitin carboxyl-terminal esterase L1 (UCHL1) is associated with stem-like cancer cell functions in pediatric high-grade glioma
Authors: PC Sanchez-Di, JC Chang, ES Moses, T Dao, Y Chen, JY Hung
PLoS ONE, 2017;12(5):e0176879.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt/?-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells
Authors: Y Kishimoto, B Ohkawara, T Sakai, M Ito, A Masuda, N Ishiguro, C Shukunami, D Docheva, K Ohno
PLoS ONE, 2017;12(7):e0182051.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnts contribute to neuromuscular junction formation through distinct signaling pathways
Authors: J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic
Development, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Surrogate Wnt agonists that phenocopy canonical Wnt and ?-catenin signalling
Authors: CY Janda, LT Dang, C You, J Chang, W de Lau, ZA Zhong, KS Yan, O Marecic, D Siepe, X Li, JD Moody, BO Williams, H Clevers, J Piehler, D Baker, CJ Kuo, KC Garcia
Nature, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/?-catenin pathway
Authors: XH Jiang, YT Xie, YP Cai, J Ren, T Ma
BMC Microbiol., 2017;17(1):124.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Galectin-1 inhibition attenuates profibrotic signaling in hypoxia-induced pulmonary fibrosis
Authors: JJ Kathiriya, N Nakra, J Nixon, PS Patel, V Vaghasiya, A Alhassani, Z Tian, D Allen-Gips, V Davé
Cell Death Discov, 2017;3(0):17010.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt3a Induces the Expression of Acetylcholinesterase during Osteoblast Differentiation via the Runx2 Transcription Factor
Authors: ML Xu, CWC Bi, EYL Liu, TTX Dong, KWK Tsim
J. Biol. Chem., 2017;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Generation of Human Liver Chimeric Mice with Hepatocytes from Familial Hypercholesterolemia Induced Pluripotent Stem Cells
Authors: J Yang, Y Wang, T Zhou, LY Wong, XY Tian, X Hong, WH Lai, KW Au, R Wei, Y Liu, LH Cheng, G Liang, Z Huang, W Fan, P Zhao, X Wang, DP Ibañez, Z Luo, Y Li, X Zhong, S Chen, D Wang, L Li, L Lai, B Qin, X Bao, AP Hutchins, CW Siu, Y Huang, MA Esteban, HF Tse
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium
Authors: N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen
Sci Rep, 2017;7(1):10741.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells
Authors: T Nakamura, T Hosoyama, J Murakami, M Samura, K Ueno, H Kurazumi, R Suzuki, A Mikamo, K Hamano
Biochem. Biophys. Res. Commun., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Aristaless Related Homeobox (ARX) Interacts with ?-Catenin, BCL9, and P300 to Regulate Canonical Wnt Signaling
Authors: IT Cho, Y Lim, JA Golden, G Cho
PLoS ONE, 2017;12(1):e0170282.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells
Authors: A Asai, E Aihara, C Watson, R Mourya, T Mizuochi, P Shivakumar, K Phelan, C Mayhew, M Helmrath, T Takebe, J Wells, JA Bezerra
Development, 2017;144(6):1056-1064.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Up-regulated NRIP2 in colorectal cancer initiating cells modulates the Wnt pathway by targeting ROR?
Authors: Z Wen, T Pan, S Yang, J Liu, H Tao, Y Zhao, D Xu, W Shao, J Wu, X Liu, Y Wang, J Mao, Y Zhu
Mol. Cancer, 2017;16(1):20.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking
Authors: K Minegishi, M Hashimoto, R Ajima, K Takaoka, K Shinohara, Y Ikawa, H Nishimura, AP McMahon, K Willert, Y Okada, H Sasaki, D Shi, T Fujimori, T Ohtsuka, Y Igarashi, TP Yamaguchi, A Shimono, H Shiratori, H Hamada
Dev. Cell, 2017;40(5):439-452.e4.
Species: Mouse
Sample Types: Whole Embryo
Applications: Bioassay -
Donor-Dependent and Other Nondefined Factors Have Greater Influence on the Hepatic Phenotype Than the Starting Cell Type in Induced Pluripotent Stem Cell Derived Hepatocyte-Like Cells
Authors: JA Heslop, R Kia, CS Pridgeon, RL Sison-Youn, T Liloglou, M Elmasry, SW Fenwick, JS Mills, NR Kitteringh, CE Goldring, BK Park
Stem Cells Transl Med, 2017;6(5):1321-1331.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
DOT1L safeguards cartilage homeostasis and protects against osteoarthritis
Authors: S Monteagudo, FMF Cornelis, C Aznar-Lope, P Yibmantasi, LA Guns, P Carmeliet, F Cailotto, RJ Lories
Nat Commun, 2017;8(0):15889.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt/?-catenin signaling contributes to tumor malignancy and is targetable in gastrointestinal stromal tumor
Authors: S Zeng, AM Seifert, JQ Zhang, MJ Cavnar, TS Kim, VP Balachandr, JA Santamaria, NA Cohen, MJ Beckman, B Medina, F Rossi, MH Crawley, JK Loo, JH Maltbaek, P Besmer, CR Antonescu, RP DeMatteo
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling
Authors: KB McCauley, F Hawkins, M Serra, DC Thomas, A Jacob, DN Kotton
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells
Authors: B Lucendo-Vi, P Filis, MJ Swortwood, MA Huestis, J Meseguer-R, K Cameron, JP Iredale, PJ O'Shaughne, PA Fowler, DC Hay
Arch. Toxicol., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Gelatin device for the delivery of growth factors involved in endochondral ossification
Authors: LA Ahrens, D Vonwil, J Christense, VP Shastri
PLoS ONE, 2017;12(4):e0175095.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/?-catenin signaling through stabilization of the WNT-receptor complex
Authors: SJ Zhao, YQ Jiang, NW Xu, Q Li, Q Zhang, SY Wang, J Li, YH Wang, YL Zhang, SH Jiang, YJ Wang, YJ Huang, XX Zhang, GA Tian, CC Zhang, YY Lv, M Dai, F Liu, R Zhang, D Zhou, ZG Zhang
Oncogene, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dishevelled stability is positively regulated by PKC?-mediated phosphorylation induced by Wnt agonists
Authors: DM Velázquez, MC Castañeda-, M Robles-Flo
Cell. Signal., 2017;35(0):107-117.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A Novel Positive Feedback Loop Between NTSR1 and Wnt/?-Catenin Contributes to Tumor Growth of Glioblastoma
Authors: H Xiao, Y Zeng, Q Wang, S Wei, X Zhu
Cell. Physiol. Biochem., 2017;43(5):2133-2142.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Multilineage communication regulates human liver bud development from pluripotency
Authors: JG Camp, K Sekine, T Gerber, H Loeffler-W, H Binder, M Gac, S Kanton, J Kageyama, G Damm, D Seehofer, L Belicova, M Bickle, R Barsacchi, R Okuda, E Yoshizawa, M Kimura, H Ayabe, H Taniguchi, T Takebe, B Treutlein
Nature, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Establishment of a refined culture method for rat colon organoids
Authors: H Isshiki, Y Arimura, K Nagaishi, K Kawakami, K Onodera, K Yamashita, Y Naishiro, M Fujimiya, K Imai, Y Shinomura
Biochem. Biophys. Res. Commun., 2017;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming
Authors: S Miura, A Suzuki
Cell Stem Cell, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Beta-catenin inhibitor BC2059 is efficacious as monotherapy or in combination with proteasome inhibitor bortezomib in multiple myeloma
Authors: I Savvidou, T Khong, A Cuddihy, C McLean, S Horrigan, A Spencer
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
AKAP2 is upregulated in ovarian cancer, and promotes growth and migration of cancer cells
Authors: X Li, C Wang, G Zhang, M Liang, B Zhang
Mol Med Rep, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells
Authors: F Ou, K Su, J Sun, W Liao, Y Yao, Y Zheng, Z Zhang
Biochem. Biophys. Res. Commun., 2017;487(2):457-463.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A process engineering approach to increase organoid yield
Authors: N Arora, J Imran Also, JW Guggenheim, M Mak, J Munera, JM Wells, RD Kamm, HH Asada, SY Shvartsman, LG Griffith
Development, 2017;144(6):1128-1136.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974
Authors: J Jang, Y Jung, Y Kim, EH Jho, Y Yoon
Sci Rep, 2017;7(0):41612.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors
Authors: S Premarathn, M Murtaza, N Matigian, LA Jolly, SA Wood
Sci Rep, 2017;7(1):8109.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System
Authors: K Low, LY Wong, M Maldonado, C Manjunath, CB Horner, M Perez, NV Myung, J Nam
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations
Authors: Q Gu, J Wang, L Wang, ZX Liu, WW Zhu, YQ Tan, WF Han, J Wu, CJ Feng, JH Fang, L Liu, L Wang, W Li, XY Zhao, BY Hu, J Hao, Q Zhou
Stem Cell Reports, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Suppression of Wnt Signaling and Osteogenic Changes in Vascular Smooth Muscle Cells by Eicosapentaenoic Acid.
Authors: Yukihiro Saito, Kazufumi Nakamura, Daiji Miura, Kei Yunoki, Toru Miyoshi, Masashi Yoshida, Norifumi Kawakita, Tomonari Kimura, Megumi Kondo, Toshihiro Sarashina, Satoshi Akagi, Atsuyuki Watanabe, Nobuhiro Nishii, Hiroshi Morita, Hiroshi Ito
Nutrients, 2017;0(0):2072-6643.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
The N-glycome of embryonic stem cells and thereof differentiated hepatic cells
Authors: H Montacir, N Freyer, F Knoespel, T Urbaniak, T Dedova, M Berger, G Damm, R Tauber, K Zeilinger, V Blanchard
Chembiochem, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity
Authors: Y Choudhury, YC Toh, J Xing, Y Qu, J Poh, L Huan, HS Tan, R Kanesvaran, H Yu, MH Tan
Sci Rep, 2017;7(0):41238.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Notch/Wnt cross-signalling regulates stemness of dental pulp stem cells through expression of neural crest and core pluripotency factors
Authors: V Uribe-Etxe, J Luzuriaga, P García-Gal, A Agliano, F Unda, G Ibarretxe
Eur Cell Mater, 2017;34(0):249-270.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro
Authors: DS Varghese, S Parween, MT Ardah, BS Emerald, SA Ansari
Stem Cells Int, 2017;2017(0):2451927.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Oncogenic retinoic acid receptor ? promotes human colorectal cancer growth through simultaneously regulating p21 transcription and GSK3?/?-catenin signaling
Authors: Gui-Li Huang
Cancer Lett, 2016;388(0):118-129.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Anthelmintic niclosamide disrupts the interplay of p65 and FOXM1/?-catenin and eradicates leukemia stem cells in chronic myelogenous leukemia
Clin Cancer Res, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration
Authors: Yasuhiro Ikeda
Stem Cells Transl Med, 2016;5(5):694-702.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling
Authors: Karthikeyan Mythreye
J. Biol. Chem., 2016;291(49):25716-25728.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2
Cancer Res, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activation of endothelial ?-catenin signaling induces heart failure
Sci Rep, 2016;6(0):25009.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins
Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Growth hormone is permissive for neoplastic colon growth
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/?-catenin defects in neuronal cholesterol synthesis phenotypes
Nat. Med., 2016;22(4):388-96.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration
Stem Cells Transl Med, 2016;5(5):694-702.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts
Authors: Elena Neumann
Matrix Biol., 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
Sci Rep, 2016;6(0):36795.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth
Authors: Ronald A DePinho
Cell, 2016;167(5):1281-1295.e18.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Active ?-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A
Authors: A Persad, G Venkateswa, L Hao, ME Garcia, J Yoon, J Sidhu, S Persad
Genes Cancer, 2016;7(12):368-382.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications
Authors: L J Spicer
Domest. Anim. Endocrinol, 2016;59(0):90-99.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay -
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation
Stem Cell Res, 2016;17(2):222-227.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Icariin attenuates titanium-particle inhibition of bone formation by activating the Wnt/?-catenin signaling pathway in vivo and in vitro.
Authors: Junhua Wang, Yunxia Tao, Zichuan Ping, Wen Zhang, Xuanyang Hu, Yijun Wang, Liangliang Wang, Jiawei Shi, Xiexing Wu, Huilin Yang, Yaozeng Xu, Dechun Geng
Scientific Reports, 2016;0(0):2045-2322.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Quantifying ?-catenin subcellular dynamics and cyclin D1 mRNA transcription during Wnt signaling in single living cells
Elife, 2016;5(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Aryl Hydrocarbon Receptor and Its Ligands Inhibit Myofibroblast Formation and Activation: Implications for Thyroid Eye Disease
Authors: Richard P Phipps
Am. J. Pathol., 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The AKAP GSKIP regulates beta-catenin through both its interactions with PKA and GSK3beta
J Biol Chem, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
Authors: Hoeke A Baarsma
J. Exp. Med, 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
TNIK inhibition abrogates colorectal cancer stemness
Nat Commun, 2016;7(0):12586.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Resveratrol ameliorates the maturation process of beta-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells.
Authors: Pezzolla D, Lopez-Beas J, Lachaud C, Dominguez-Rodriguez A, Smani T, Hmadcha A, Soria B
PLoS ONE, 2015;10(3):e0119904.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
Authors: Huang S, Green M, de Carvalho A, Mumau M, Chen Y, D'Souza S, Snoeck H
Nat Protoc, 2015;10(3):413-25.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note.
Authors: James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, Dennison A, Metcalfe M, Garcea G, Lloyd D, Berry D, Steward W, Brown K
PLoS ONE, 2015;10(2):e0117776.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis.
Authors: Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, Gundry R, Tomita-Mitchell A, Lough J
PLoS ONE, 2015;10(2):e0118670.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Downregulation of the Wnt inhibitor CXXC5 predicts a better prognosis in acute myeloid leukemia.
Authors: Kuhnl A, Valk P, Sanders M, Ivey A, Hills R, Mills K, Gale R, Kaiser M, Dillon R, Joannides M, Gilkes A, Haferlach T, Schnittger S, Duprez E, Linch D, Delwel R, Lowenberg B, Baldus C, Solomon E, Burnett A, Grimwade D
Blood, 2015;125(19):2985-94.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A protein kinase A (PKA)/beta-catenin pathway sustains the BMP2/DLX3-induced osteogenic differentiation in dental follicle cells (DFCs).
Authors: Viale-Bouroncle S, Klingelhoffer C, Ettl T, Reichert T, Morsczeck C
Cell Signal, 2015;27(3):598-605.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Canonical Wnt pathway inhibitor ICG-001 induces cytotoxicity of multiple myeloma cells in Wnt-independent manner.
Authors: Grigson E, Ozerova M, Pisklakova A, Liu H, Sullivan D, Nefedova Y
PLoS ONE, 2015;10(1):e0117693.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Hepcidin regulation in prostate and its disruption in prostate cancer.
Authors: Tesfay L, Clausen K, Kim J, Hegde P, Wang X, Miller L, Deng Z, Blanchette N, Arvedson T, Miranti C, Babitt J, Lin H, Peehl D, Torti F, Torti S
Cancer Res, 2015;75(11):2254-63.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Focal adhesion kinase and Wnt signaling regulate human ductal carcinoma in situ stem cell activity and response to radiotherapy.
Authors: Williams K, Bundred N, Landberg G, Clarke R, Farnie G
Stem Cells, 2015;33(2):327-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A Regulatory Network Involving beta-Catenin, e-Cadherin, PI3k/Akt, and Slug Balances Self-Renewal and Differentiation of Human Pluripotent Stem Cells In Response to Wnt Signaling.
Authors: Huang T, Li L, Moalim-Nour L, Jia D, Bai J, Yao Z, Bennett S, Figeys D, Wang L
Stem Cells, 2015;33(5):1419-33.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
Nat Commun, 2015;6(0):6156.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Endogenously produced Indian Hedgehog regulates TGFbeta-driven chondrogenesis of human bone marrow stromal/stem cells.
Authors: Handorf A, Chamberlain C, Li W
Stem Cells Dev, 2015;24(8):995-1007.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Intestinal Commitment and Maturation of Human Pluripotent Stem Cells Is Independent of Exogenous FGF4 and R-spondin1.
Authors: Tamminen K, Balboa D, Toivonen S, Pakarinen M, Wiener Z, Alitalo K, Otonkoski T
PLoS ONE, 2015;10(7):e0134551.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Transcriptome analysis of wnt3a-treated triple-negative breast cancer cells.
Authors: Maubant S, Tesson B, Maire V, Ye M, Rigaill G, Gentien D, Cruzalegui F, Tucker G, Roman-Roman S, Dubois T
PLoS ONE, 2015;10(4):e0122333.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
Nat Protoc, 2015;10(3):363-81.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Novel cancer chemotherapy hits by molecular topology: dual Akt and Beta-catenin inhibitors.
Authors: Zanni R, Galvez-Llompart M, Morell C, Rodriguez-Henche N, Diaz-Laviada I, Recio-Iglesias M, Garcia-Domenech R, Galvez J
PLoS ONE, 2015;10(4):e0124244.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Transcription factor binding dynamics during human ES cell differentiation.
Authors: Tsankov, Alexande, Gu, Hongcang, Akopian, Veronika, Ziller, Michael, Donaghey, Julie, Amit, Ido, Gnirke, Andreas, Meissner, Alexande
Nature, 2015;518(7539):344-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.
Authors: Iyer D, Gambardella L, Bernard W, Serrano F, Mascetti V, Pedersen R, Talasila A, Sinha S
Development, 2015;142(8):1528-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
microRNA-103/107 Family Regulates Multiple Epithelial Stem Cell Characteristics.
Authors: Peng H, Park J, Katsnelson J, Kaplan N, Yang W, Getsios S, Lavker R
Stem Cells, 2015;33(5):1642-56.
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Epsin is required for Dishevelled stability and Wnt signalling activation in colon cancer development.
Authors: Chang B, Tessneer K, McManus J, Liu X, Hahn S, Pasula S, Wu H, Song H, Chen Y, Cai X, Dong Y, Brophy M, Rahman R, Ma J, Xia L, Chen H
Nat Commun, 2015;6(0):6380.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tissue-engineered vascular grafts created from human induced pluripotent stem cells.
Authors: Sundaram S, One J, Siewert J, Teodosescu S, Zhao L, Dimitrievska S, Qian H, Huang A, Niklason L
Stem Cells Transl Med, 2014;3(12):1535-43.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of beta-catenin through mechanisms involving FRAT1 and PAK4.
Authors: Jin R, Liu W, Menezes S, Yue F, Zheng M, Kovacevic Z, Richardson D
J Cell Sci, 2014;127(0):3116-30.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer.
Authors: Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X
Nat Commun, 2014;5(0):3149.
-
miR-99a/100~125b tricistrons regulate hematopoietic stem and progenitor cell homeostasis by shifting the balance between TGFbeta and Wnt signaling.
Authors: Emmrich S, Rasche M, Schoning J, Reimer C, Keihani S, Maroz A, Xie Y, Li Z, Schambach A, Reinhardt D, Klusmann J
Genes Dev, 2014;28(8):858-74.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
High efficient differentiation of functional hepatocytes from porcine induced pluripotent stem cells.
Authors: Ao, Ying, Mich-Basso, Jocelyn, Lin, Bo, Yang, Lei
PLoS ONE, 2014;9(6):e100417.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
Cell Stem Cell, 2014;14(3):342-56.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
sRNA-seq analysis of human embryonic stem cells and definitive endoderm reveals differentially expressed microRNAs and novel IsomiRs with distinct targets.
Authors: Hinton A, Hunter S, Afrikanova I, Jones G, Lopez A, Fogel G, Hayek A, King C
Stem Cells, 2014;32(9):2360-72.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Whole-exome sequencing reveals LRP5 mutations and canonical Wnt signaling associated with hepatic cystogenesis.
Authors: Cnossen W, te Morsche R, Hoischen A, Gilissen C, Chrispijn M, Venselaar H, Mehdi S, Bergmann C, Veltman J, Drenth J
Proc Natl Acad Sci U S A, 2014;111(14):5343-8.
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Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells.
Authors: Lin, I-Ying, Chiu, Feng-Lan, Yeang, Chen-Hsi, Chen, Hsin-Fu, Chuang, Ching-Yu, Yang, Shii-Yi, Hou, Pei-Shan, Sintupisut, Nardnisa, Ho, Hong-Ner, Kuo, Hung-Chi, Lin, Kuo-I
Stem Cell Reports, 2014;2(2):189-204.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
J Immunol, 2014;193(1):439-51.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway.
Authors: Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J
Exp Cell Res, 2014;322(2):355-64.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
Mol Cancer, 2014;13(0):88.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Canonical Wnt signaling skews TGF-beta signaling in chondrocytes towards signaling via ALK1 and Smad 1/5/8.
Authors: van den Bosch M, Blom A, van Lent P, van Beuningen H, Blaney Davidson E, van der Kraan P, van den Berg W
Cell Signal, 2014;26(5):951-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A synthetic peptide-acrylate surface for production of insulin-producing cells from human embryonic stem cells.
Authors: Lin P, Hung S, Yang Y, Liao L, Hsieh Y, Yen H, Lu H, Lee M, Chu I, Hwang S
Stem Cells Dev, 2014;23(4):372-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
PLoS ONE, 2014;9(8):e104937.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo engraftment.
Authors: Hwang Y, Suk S, Shih Y, Seo T, Du B, Xie Y, Li Z, Varghese S
Sci Rep, 2014;4(0):5916.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Polycomb group protein expression during differentiation of human embryonic stem cells into pancreatic lineage in vitro.
Authors: Pethe, Prasad, Nagvenkar, Punam, Bhartiya, Deepa
BMC Cell Biol, 2014;15(0):18.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pigment epithelium-derived factor 34-mer peptide prevents liver fibrosis and hepatic stellate cell activation through down-regulation of the PDGF receptor.
Authors: Tsai T, Shih S, Ho T, Ma H, Liu M, Chen S, Tsao Y
PLoS ONE, 2014;9(4):e95443.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Wnt/beta-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway.
Authors: Peng X, Yang L, Chang H, Dai G, Wang F, Duan X, Guo L, Zhang Y, Chen G
PLoS ONE, 2014;9(5):e97283.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
Nat Commun, 2014;5(0):4806.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt-dependent T-cell factor-4 controls human etravillous trophoblast motility.
Authors: Meinhardt G, Haider S, Haslinger P, Proestling K, Fiala C, Pollheimer J, Knofler M
Endocrinology, 2014;155(5):1908-20.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling.
Authors: Nakamura I, Zakharia K, Banini B, Mikhail D, Kim T, Yang J, Moser C, Shaleh H, Thornburgh S, Walters I, Roberts L
PLoS ONE, 2014;9(4):e92273.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.
Authors: Carmon K, Gong X, Yi J, Thomas A, Liu Q
Proc Natl Acad Sci U S A, 2014;111(13):E1221-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Influence of in vitro and in vivo oxygen modulation on beta cell differentiation from human embryonic stem cells.
Authors: Cechin S, Alvarez-Cubela S, Giraldo J, Molano R, Villate S, Ricordi C, Pileggi A, Inverardi L, Fraker C, Dominguez-Bendala J
Stem Cells Transl Med, 2014;3(3):277-89.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A stable and reproducible human blood-brain barrier model derived from hematopoietic stem cells.
Authors: Cecchelli R, Aday S, Sevin E, Almeida C, Culot M, Dehouck L, Coisne C, Engelhardt B, Dehouck M, Ferreira L
PLoS ONE, 2014;9(6):e99733.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation of bladder urothelium from human pluripotent stem cells under chemically defined serum- and feeder-free system.
Authors: Kang, Minyong, Kim, Hyeon Ho, Han, Yong-Mah
Int J Mol Sci, 2014;15(5):7139-57.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
Authors: Kilander M, Dahlstrom J, Schulte G
Cell Signal, 2014;26(9):1943-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An investigation of the effects of the core protein telomerase reverse transcriptase on Wnt signaling in breast cancer cells.
Authors: Listerman I, Gazzaniga F, Blackburn E
Mol Cell Biol, 2014;34(2):280-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Efficient programming of human eye conjunctiva-derived induced pluripotent stem (ECiPS) cells into definitive endoderm-like cells.
Authors: Massumi M, Hoveizi E, Baktash P, Hooti A, Ghazizadeh L, Nadri S, Pourasgari F, Hajarizadeh A, Soleimani M, Nabiuni M, Khorramizadeh M
Exp Cell Res, 2014;322(1):51-61.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers.
Authors: Lam A, Freedman B, Morizane R, Lerou P, Valerius M, Bonventre J
J Am Soc Nephrol, 2014;25(6):1211-25.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-1beta-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA.
Authors: Lee J, Heur M
Mol Cell Biol, 2014;34(18):3535-45.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
beta-catenin promotes the type I IFN synthesis and the IFN-dependent signaling response but is suppressed by influenza A virus-induced RIG-I/NF-kappaB signaling.
Authors: Hillesheim A, Nordhoff C, Boergeling Y, Ludwig S, Wixler V
Cell Commun Signal, 2014;12(0):29.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Glycogen synthase kinase 3 inhibitors induce the canonical WNT/beta-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma.
Authors: Chen E, DeRan M, Ignatius M, Grandinetti K, Clagg R, McCarthy K, Lobbardi R, Brockmann J, Keller C, Wu X, Langenau D
Proc Natl Acad Sci U S A, 2014;111(14):5349-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Differentiation of human pluripotent stem cells into nephron progenitor cells in a serum and feeder free system.
Authors: Kang, Minyong, Han, Yong-Mah
PLoS ONE, 2014;9(4):e94888.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Nodal.Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation.
Authors: Fuerer C, Nostro M, Constam D
J Biol Chem, 2014;289(25):17854-71.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium.
Authors: Jabaji Z, Brinkley G, Khalil H, Sears C, Lei N, Lewis M, Stelzner M, Martin M, Dunn J
PLoS ONE, 2014;9(9):e107814.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Drugging a stem cell compartment using Wnt3a protein as a therapeutic.
Authors: Dhamdhere, Girija R, Fang, Mark Y, Jiang, Jie, Lee, Katherin, Cheng, Du, Olveda, Rebecca, Liu, Bo, Mulligan, Kimberle, Carlson, Jeffery, Ransom, Ryan C, Weis, William, Helms, Jill A
PLoS ONE, 2014;9(1):e83650.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Zrf1 is required to establish and maintain neural progenitor identity.
Authors: Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L
Genes Dev, 2014;28(2):182-97.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Protein kinase C delta negatively modulates canonical Wnt pathway and cell proliferation in colon tumor cell lines.
Authors: Hernandez-Maqueda J, Luna-Ulloa L, Santoyo-Ramos P, Castaneda-Patlan M, Robles-Flores M
PLoS ONE, 2013;8(3):e58540.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer.
Authors: Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W
J Biol Chem, 2013;288(36):26301-10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation of multipotent foregut stem cells from human pluripotent stem cells.
Authors: Hannan, Nicholas, Fordham, Robert P, Syed, Yasir A, Moignard, Victoria, Berry, Andrew, Bautista, Ruben, Hanley, Neil A, Jensen, Kim B, Vallier, Ludovic
Stem Cell Reports, 2013;1(4):293-306.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
Mol Ther, 2013;21(1):228-39.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Activation of the Wnt pathway through AR79, a GSK3beta inhibitor, promotes prostate cancer growth in soft tissue and bone.
Authors: Jiang Y, Dai J, Zhang H, Sottnik J, Keller J, Escott K, Sanganee H, Yao Z, McCauley L, Keller E
Mol Cancer Res, 2013;11(12):1597-610.
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Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer.
Authors: Tahir S, Yang G, Goltsov A, Song K, Ren C, Wang J, Chang W, Thompson T
Cancer Res, 2013;73(6):1900-11.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors.
Cell Signal, 2013;25(1):178-89.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IQGAP1 protein regulates nuclear localization of beta-catenin via importin-beta5 protein in Wnt signaling.
Authors: Goto T, Sato A, Adachi S, Iemura S, Natsume T, Shibuya H
J Biol Chem, 2013;288(51):36351-60.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Atypical protein kinase Ciota is required for Wnt3a-dependent neurite outgrowth and binds to phosphorylated dishevelled 2.
Authors: Greer Y, Fields A, Brown A, Rubin J
J Biol Chem, 2013;288(13):9438-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo.
Authors: Rezania A, Bruin J, Xu J, Narayan K, Fox J, O'Neil J, Kieffer T
Stem Cells, 2013;31(11):2432-42.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The Wnt signalling pathway is upregulated in an in vitro model of acquired tamoxifen resistant breast cancer.
Authors: Loh Y, Hedditch E, Baker L, Jary E, Ward R, Ford C
BMC Cancer, 2013;13(0):174.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Derivation of cerebellar neurons from human pluripotent stem cells.
Authors: Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S
Curr Protoc Stem Cell Biol, 2012;20(0):1H.51.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
Cell Transplant, 2012;21(6):1321-39.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells.
Authors: Sui L, Mfopou J, Geens M, Sermon K, Bouwens L
Biochem Biophys Res Commun, 2012;426(3):380-5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt5a and Wnt11 are essential for second heart field progenitor development.
Authors: Cohen E, Miller M, Wang Z, Moon R, Morrisey E
Development, 2012;139(11):1931-40.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
Blood, 2012;119(7):1683-92.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems.
Authors: Aad, Pauline, Echternkamp, Sherrill, Sypherd, David D, Schreiber, Nicole B, Spicer, Leon J
Biol Reprod, 2012;87(4):79.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay -
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Authors: Spence JR, Mayhew CN, Rankin SA
Nature, 2011;470(7332):105-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis.
Authors: Kohler EE, Cowan CE, Chatterjee I
Blood, 2011;117(5):1761-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
J. Bone Miner. Res., 2011;26(9):2082-95.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Evidence for altered Wnt signaling in psoriatic skin.
Authors: Gudjonsson JE, Johnston A, Stoll SW
J. Invest. Dermatol., 2010;130(7):1849-59.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Canonical Wnts function as potent regulators of osteogenesis by human mesenchymal stem cells.
Authors: Liu G, Vijayakumar S, Grumolato L, Arroyave R, Qiao H, Akiri G, Aaronson SA
J. Cell Biol., 2009;185(1):67-75.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion.
Authors: Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C
Oncotarget, 0;6(37):40310-26.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) at a different concentration than recommended?
We cannot guarantee the performance if the protein is reconstituted at a different concentration than recommended in the product datasheet. Please contact Technical Service if you have any questions or concerns about reconstitution.
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Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) in a different buffer than recommended?
For best recovery, stability, and activity, the protein should be reconstituted in the buffer recommended on the product datasheet. Stability testing has been evaluated under these conditions; we can support the performance under these reconstitution conditions.
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HepG2 cells were treated with 100 ng/ml Wnt3a for 2 days, following which the LDH activity of cell culture medium samples were assayed. The result shows that Wnt3a increased LDH release in HepG2 cells.
Reason for Rating: Recombinant Wnt3a product was added to activate Wnt/ beta -catenin signaling pathway.
Reason for Rating: This protein (200 ng/mL) works well for activating Wnt signaling as shown by Topflash luciferase activity.
Reason for Rating: The product worked to activate Wnt signaling in breast cancer cells with a Wnt reporter, however performed less well than supernatants from L cell Wnt3a lines.
Recombinant Wnt3a product was added at 100 ng/mL for 2 days on 7TGC reporter cell lines. Activation was measured with GFP levels by Flow.
Cell motility assay performed with MA-11 cells untreated (A) and treated (B) for 72 hours with R&D Recombinant Human Wnt-3a (#5036-WN/CF) at 250 ng/mL.
We use for stem cell maintenance and differentiation.
We use this product for our neuron stem cells studies.
Reason for Rating: It works well.
I tested the activity of the recombinant protein in the HEK293 Top Flash Reporter cell lines. LiCl was used as a positive control.
Luciferase reporter activity by TOPFlash/FOPFlash for Wnt/B-catenin activity was performed in cultured cells. Cells were transfected for 48 hours with plasmid and treated with Wnt3a for 24 hours prior to performing Dual-Glo luciferase assay.
Reason for Rating: We attempted to use the product to induce skeletal muscle differentiation in Mus musculus stem cells, but our results suggest differentiation to cardiomyocytes.
Reason for Rating: I have used Wnt3a routinely to stimulate primary cells in culture. I have obtained consistent results with reproducible data.
Reason for Rating: This protein works very well for activating Wnt signaling as shown by B-catenin expression.