Recombinant Human NPEPPS Protein, CF

R&D Systems | Catalog # 6410-ZN

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human NPEPPS Protein (6410-ZN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

P55786

Applications

Enzyme Activity
View all Puromycin-sensitive aminopeptidase/NPEPPS Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Puromycin-sensitive aminopeptidase/NPEPPS protein
Pro46-Val919, with an N-terminal Met and a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro46

Predicted Molecular Mass

100 kDa

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC.
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

6410-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Puromycin-sensitive aminopeptidase/NPEPPS

NPEPPS, also known as Puromycin-sensitive aminopeptidase and cytosol alanyl aminopeptidase, is a zinc metallopeptidase which hydrolyzes N‑terminal amino acids from its substrates. NPEPPS is expressed in most tissues as a cytoplasmic protein, but a membrane-associated form has been identified in the brain (1, 2). The enzyme has broad substrate specificity but prefers basic and hydrophobic residues in the substrate P1 and P'1 sites (3). NPEPPS is known to hydrolyze dynorphins and enkephalins, and may contribute to the degradation of these peptides in the brain (4). NPEPPS plays a major role in the degradation of toxic polyglutaminyl peptides that are released by proteasomes (5). NPEPPS is also known to degrade the tau protein, which accumulates and polymerizes in some neurodegenerative diseases (6).

References

  1. Dyer, S.H. et al. (1990) J. Neurochem. 54:547.
  2. McLellan, S. et al. (1988) J. Neurochem. 51:1552.
  3. Johnson, G.D. and L.B. Hersh (1990) Arch. Biochem. Biophys. 276:305.
  4. Safavi, A. and L.B. Hersh (1995) J. Neurochem. 65:389.
  5. Bhutani, N. et al. (2007) EMBO J. 26:1385.
  6. Sengupta, S. et al. (2006) Biochemistry 45:15111.

Alternate Names

AAP-S, MP100, NPEPPS

Entrez Gene IDs

9520 (Human)

Gene Symbol

NPEPPS

UniProt

P55786

Additional Puromycin-sensitive aminopeptidase/NPEPPS Products

Product Documents for Recombinant Human NPEPPS Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human NPEPPS Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Recombinant Human NPEPPS Protein, CF

Customer Reviews for Recombinant Human NPEPPS Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human NPEPPS Protein, CF and earn rewards!

Have you used Recombinant Human NPEPPS Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human NPEPPS Protein, CF (6410-ZN):

Materials
  • Assay Buffer: 25 mM HEPES, 1 mM DTT, pH 7.0
  • Recombinant Human Puromycin‑sensitive aminopeptidase/NPEPPS (rhNPEPPS) (Catalog # 6410-ZN)
  • Substrate: Leu-AMC (Bachem, Catalog # I-1240), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhNPEPPS to 0.2 ng/μL in Assay Buffer.
  2. Dilute Substrate to 20 μM in Assay Buffer.
  3. Load 50 μL of 0.2 ng/μL rhNPEPPS into the microplate and start the reaction by adding 50 μL of Substrate. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, for 5 minutes in kinetic mode.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891.

Per Well:
  • rhNPEPPS: 0.010 µg
  • Substrate: 10 µM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes