Recombinant Human NPEPPS Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Product Details
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Recombinant Human NPEPPS Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC. The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Puromycin-sensitive aminopeptidase/NPEPPS protein
Pro46-Val919, with an N-terminal Met and a C-terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
100 kDa
90-100 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 25 mM HEPES, 1 mM DTT, pH 7.0
  • Recombinant Human Puromycin‑sensitive aminopeptidase/NPEPPS (rhNPEPPS) (Catalog # 6410-ZN)
  • Substrate: Leu-AMC (Bachem, Catalog # I-1240), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhNPEPPS to 0.2 ng/μL in Assay Buffer.
  2. Dilute Substrate to 20 μM in Assay Buffer.
  3. Load 50 μL of 0.2 ng/μL rhNPEPPS into the microplate and start the reaction by adding 50 μL of Substrate. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, for 5 minutes in kinetic mode.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891.

Per Well:
  • rhNPEPPS: 0.010 µg
  • Substrate: 10 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Puromycin-sensitive aminopeptidase/NPEPPS

NPEPPS, also known as Puromycin-sensitive aminopeptidase and cytosol alanyl aminopeptidase, is a zinc metallopeptidase which hydrolyzes N‑terminal amino acids from its substrates. NPEPPS is expressed in most tissues as a cytoplasmic protein, but a membrane-associated form has been identified in the brain (1, 2). The enzyme has broad substrate specificity but prefers basic and hydrophobic residues in the substrate P1 and P'1 sites (3). NPEPPS is known to hydrolyze dynorphins and enkephalins, and may contribute to the degradation of these peptides in the brain (4). NPEPPS plays a major role in the degradation of toxic polyglutaminyl peptides that are released by proteasomes (5). NPEPPS is also known to degrade the tau protein, which accumulates and polymerizes in some neurodegenerative diseases (6).

  1. Dyer, S.H. et al. (1990) J. Neurochem. 54:547.
  2. McLellan, S. et al. (1988) J. Neurochem. 51:1552.
  3. Johnson, G.D. and L.B. Hersh (1990) Arch. Biochem. Biophys. 276:305.
  4. Safavi, A. and L.B. Hersh (1995) J. Neurochem. 65:389.
  5. Bhutani, N. et al. (2007) EMBO J. 26:1385.
  6. Sengupta, S. et al. (2006) Biochemistry 45:15111.
Entrez Gene IDs
9520 (Human)
Alternate Names
AAP-S; aminopeptidase puromycin sensitive; Cytosol alanyl aminopeptidase; EC; EC; metalloproteinase MP100; MP100; MP100EC 3.4.11; NPEPPS; PSAEC; puromycin-sensitive aminopeptidase

Product Specific Notices

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

Citation for Recombinant Human NPEPPS Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells.
    Authors: Dasgupta S, Castro L, Dulman R, Yang C, Schmidt M, Ferro E, Fricker L
    PLoS ONE, 2014;9(7):e103604.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay


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