Recombinant Human NRG1-alpha/HRG1-alpha EGF Domain Protein

Carrier Free

Catalog # Availability Size / Price Qty
296-HR-050/CF

With Carrier

Catalog # Availability Size / Price Qty
296-HR-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (11)
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Recombinant Human NRG1-alpha/HRG1-alpha EGF Domain Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 10‑50 ng/mL.
Source
E. coli-derived human Neuregulin-1 alpha/NRG1 alpha protein
Ser177-Lys241
Accession #
N-terminal Sequence
Analysis
Ser177
Predicted Molecular Mass
7 kDa
SDS-PAGE
8 kDa, reducing conditions

Product Datasheets

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296-HR (with carrier)

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296-HR/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

296-HR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

296-HR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuregulin-1 alpha/NRG1 alpha

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 encodes more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. The alpha - or  beta -splice variants differ in their C-terminal region. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF). NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1 (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and to promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

References
  1. Lemke, G. (2006) Science STKE 325:pe11.
  2. Esper, R.M. et al. (2006) Brain Res. Brain Res. Rev. 51:161.
  3. Tosato, S. et al. (2005) Schizophr. Bull. 31:613.
Entrez Gene IDs
3084 (Human); 211323 (Mouse); 112400 (Rat)
Alternate Names
ARIA; GGF2; HGL; HRG; HRG1; MST131; MSTP131; neuregulin 1; Neuregulin1 alpha; Neuregulin-1 alpha; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF

Citations for Recombinant Human NRG1-alpha/HRG1-alpha EGF Domain Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. A novel anti-HER2 antibody GB235 reverses Trastuzumab resistance in HER2-expressing tumor cells in vitro and in vivo
    Authors: M Shu, H Yan, C Xu, Y Wu, Z Chi, W Nian, Z He, J Xiao, H Wei, Q Zhou, JX Zhou
    Sci Rep, 2020;10(1):2986.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Modulation of the Neuregulin 1/ErbB system after skeletal muscle denervation and reinnervation
    Authors: M Morano, G Ronchi, V Nicolò, BE Fornasari, A Crosio, I Perroteau, S Geuna, G Gambarotta, S Raimondo
    Sci Rep, 2018;8(1):5047.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Neuregulin1 alpha activates migration of neuronal progenitors expressing ErbB4
    Authors: Benedetta Elena Fornasari
    Mol. Cell. Neurosci, 2016;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Neuregulin-1 attenuates mortality associated with experimental cerebral malaria.
    Authors: Solomon W, Wilson N, Anderson L, Pitts S, Patrickson J, Liu M, Ford B, Stiles J
    J Neuroinflammation, 2014;11(0):9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. HER3 targeting of adenovirus by fiber modification increases infection of breast cancer cells in vitro, but not following intratumoral injection in mice.
    Authors: MacLeod S, Elgadi M, Bossi G, Sankar U, Pisio A, Agopsowicz K, Sharon D, Graham F, Hitt M
    Cancer Gene Ther, 2012;19(12):888-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2007;282(1):721-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
    Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
    J. Biol. Chem., 2007;282(37):26656-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A system for quantifying dynamic protein interactions defines a role for Herceptin in modulating ErbB2 interactions.
    Authors: Wehrman TS, Raab WJ, Casipit CL, Doyonnas R, Pomerantz JH, Blau HM
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(50):19063-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Prolactin and heregulin override DNA damage-induced growth arrest and promote phosphatidylinositol-3 kinase-dependent proliferation in breast cancer cells.
    Authors: Chakravarti P, Henry MK, Quelle FW
    Int. J. Oncol., 2005;26(2):509-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Neuregulin-1 is neuroprotective and attenuates inflammatory responses induced by ischemic stroke.
    Authors: Xu Z, Jiang J, Ford G, Ford BD
    Biochem. Biophys. Res. Commun., 2004;322(2):440-6.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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