Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein

(10 citations)
(1 Review)
  
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 2.5-12.5 ng/mL.
  • Source
    E. coli-derived Ser2-Lys246
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser2
  • Predicted Molecular Mass
    26.9 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
377-HB
 
377-HB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) stimulates cell proliferation of the MCF-7 human breast cancer cell line in a serum free assay. The ED50 for this effect is 2.5-12.5 ng/mL.
1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.
Background: Neuregulin-1 beta 1/NRG1 beta 1

The Neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

  • References:
    1. Buonanno, A., and G.D Fischbach (2001) Curr. Opin. Neurobiol. 11:287.
    2. Adlkofer, K. and C. Lai (2000) Glia 29:104.
    3. Garratt, A.N. et al., (2000) BioEssays 22:987.
  • Entrez Gene IDs:
    3084 (Human); 211323 (Mouse); 112400 (Rat)
  • Alternate Names:
    Neuregulin1 beta 1; Neuregulin-1 beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Identification of Anti-EGFR and Anti-ErbB3 Dual Variable Domains Immunoglobulin (DVD-Ig) Proteins with Unique Activities.
    Authors: Gu J, Yang J, Chang Q, Liu Z, Ghayur T, Gu J
    PLoS ONE, 2015;10(5):e0124135.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury.
    Authors: Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M
    Stem Cells Transl Med, 2014;3(3):334-45.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  3. Cellular Effects of HER3-Specific Affibody Molecules.
    Authors: Gostring L, Malm M, Hoiden-Guthenberg I, Frejd FY, Stahl S, Lofblom J, Gedda L
    PLoS ONE, 2012;7(6):e40023.
    Species: Human
    Sample Type: Buffer
    Application: Binding Assay
  4. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells.
    Authors: Sheng, Qing, Liu, Xinggang, Fleming, Eleanor, Yuan, Karen, Piao, Huiying, Chen, Jinyun, Moustafa, Zeinab, Thomas, Roman K, Greulich, Heidi, Schinzel, Anna, Zaghlul, Sara, Batt, David, Ettenberg, Seth, Meyerson, Matthew, Schoeberl, Birgit, Kung, Andrew L, Hahn, William, Drapkin, Ronny, Livingston, David M, Liu, Joyce F
    Cancer Cell, 2010;17(3):298-310.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice.
    Authors: Casazza A, Finisguerra V, Capparuccia L
    J. Clin. Invest., 2010;120(8):2684-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. ErbB2 directly activates the exchange factor Dock7 to promote Schwann cell migration.
    Authors: Yamauchi J, Miyamoto Y, Chan JR, Tanoue A
    J. Cell Biol., 2008;181(2):351-65.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  8. Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
    Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
    Cancer Cell, 2006;10(1):39-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006;15(3):407-21.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  10. Neuregulin-1-beta1 enters brain and spinal cord by receptor-mediated transport.
    Authors: Kastin AJ, Akerstrom V, Pan W
    J. Neurochem., 2004;88(4):965-70.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
Average Rating: 5 (Based on 1 review)

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Other: Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein [377-HB]

Summary

ApplicationOther

Other Experimental Details

Other Experimental DetailsUsed for Schwann cell culture studies

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