Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10261-OL-050
Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF SDS-PAGE
1 Image
Product Details
FAQs
Reviews

Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the spreading of NIH3T3 mouse embryonic fibroblast cells Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) immobilized at 0.5-1 μg/mL will significantly support cell spreading.
Source
Chinese Hamster Ovary cell line, CHO-derived human Olfactomedin-4/OLFM4 protein
Asp21-Gln510, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp21
Predicted Molecular Mass

56 kDa

SDS-PAGE
66-75 kDa, under reducting conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10261-OL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Olfactomedin-4/OLFM4 His-tag (Catalog # 10261-OL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-75 kDa on reducing gel.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Olfactomedin-4/OLFM4

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF stimulated clone-1) is an approximately 64-72 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of protein (1-3). It is expressed in epithelial cells of the prostate, small intestine, colon, bone marrow, and in several cancers (4-7), and is up-regulated in epithelial cells during inflammation (8). Mature OLFM4 is a 490 amino acid (aa) protein that is secreted mainly in a polymeric form which is held together by disulfide bonds and carbohydrate interactions (2). Mature human OLFM4 shares 71% aa sequence identity with mature mouse and rat OLFM4. Olfactomedin-4 (OLFM4) interacts with cell surface lectins and cadherins, which helps cell adhesion and cell spreading of NIH3T3 and 293T/17 cells (9). OLFM4 plays a critical role in regulating progression of prostate cancer and is also novel biomarker for triple-negative breast cancer (10-11).

References
  1. Zhang, J. et al. (2002) Gene 283:83.
  2. Grover, P.K. et al. (2010) Cancer Metastasis Rev. 29:761.
  3. Guette, C. et al. (2015) Proteomics Clin. Appl. 9:58.
  4. Zhang, X. et al. (2004) Cancer Res. 64:2474.
  5. Kobayashi, D. et al. (2007) Cancer Sci. 98:334.
  6. Koshida, S. et al. (2007) Cancer Sci. 98:315.
  7. Yu, L. et al. (2011) Int. J. Gynecol. Cancer 21:367.
  8. Shinozaki, S. et al. (2001) Gut 48:623.
  9. Liu, W. et al. (2006) Exp. Cell Res. 312:1785.
  10. Xiong, B. et al. (2017) Biomed. Pharmacother. 86:67.
  11. Li, H. et al. (2015) Sci. Rep. 5:16974.
Entrez Gene IDs
10562 (Human); 380924 (Mouse); 290409 (Rat)
Alternate Names
Antiapoptotic protein GW112; bA209J19.1; GC1; G-CSF-stimulated clone 1 protein; GW112; GW112hOLfD; hGC-1; KIAA4294; olfactomedin 4; Olfactomedin-4; OlfD; OLFM4; OLM4; UNQ362

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF and earn rewards!

Have you used Recombinant Human Olfactomedin-4/OLFM4 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review