Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10059-TA-050
Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF Bioactivity
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Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 2-10 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human OLT-2/TARM1 protein
Human OLT-2/TARM1
(Gln17-Val235)
Accession # NP_001129158.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln17 inferred from enzymatic pyroglutamate treatment revealing Gly18.
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa
SDS-PAGE
64-74 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10059-TA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human OLT-2/TARM1 Fc Chimera (Catalog # 10059-TA) inhibits IFN gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 2-10 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human OLT-2/TARM1 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 64-74 kDa and 130- 150 kDa,  respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OLT-2/TARM1

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4+ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

References
  1. Radjabova, V., et al. (2015) J Immunol. 195:3149.
Long Name
T-cell-interacting Activating Receptor on Myeloid Cells 1
Entrez Gene IDs
441864 (Human); 245126 (Mouse); 499065 (Rat)
Alternate Names
OLT2; OLT-2/TARM1; OSCAR-Like Transcript-2 Protein; T Cell-Interacting, Activating Receptor On Myeloid Cells 1; T Cell-Interacting, Activating Receptor On Myeloid Cells; TARM1; T-Cell-Interacting, Activating Receptor On Myeloid Cells Protein 1

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