Recombinant Human Oncostatin M (HEK-293-expressed) Protein

R&D Systems | Catalog # 8475-OM

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Oncostatin M (HEK-293-expressed) Protein (8475-OM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P13725

Applications

Bioactivity
View all Oncostatin M/OSM Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Oncostatin M/OSM protein
Ala26-Arg221

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala26 & Ile28

Predicted Molecular Mass

22 kDa

SDS-PAGE

27-35 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.05-0.3 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 8475-OM in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8475-OM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8475-OM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Oncostatin M/OSM

Oncostatin M (OSM) is an approximately 30 KDa secreted cytokine belonging to the Interleukin-6 family. Like other members of the IL-6 family such as IL-11, CNTF, and Cardiotrophin-1, OSM plays crucial roles in inflammation, neuroprotection, hematopoiesis, metabolism and development (1). The human OSM cDNA encodes a 252 amino acid (aa) prepro protein with a signal peptide and a hydrophilic Cterminal domain that are proteolytically processed to generate the 195 aa mature form (2). Although both mature and pro-OSM are equally active in radioreceptor assays, the mature OSM is 5 to 60 fold more active in growth inhibition assays (3). Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM (3). Cytokines of the IL-6 family share a common receptor subunit, gp130, which allows intracellular signal transduction. In contrast to other cytokines, OSM binds gp130 with very low affinity and has little to no biological activity unless a second receptor chain is recruited (4). The OSM-gp130 complex recruits either LIF R alpha, or OSM R beta to form the OSM receptor type I or OSM receptor type II respectively (5). Among multiples roles, OSM has been reported to modulate tumor cell and epithelial cell growth (6, 7). An axis involving OSM, CD40 Ligand and OSM receptor described as an antiviral and immunostimulatory system is disrupted in patients liver showing hepatitis C infection (8). Human OSM shares 45% aa sequence homology with mouse and rat OSM.

References

  1. Richards, C.D. (2013) ISRN Inflamm. 2013:512103.
  2. Malik, N. et al. (1989) Mol. Cel. Biol. 9:2847.
  3. Linsley, P.S. et al. (1990) Mol. Cel. Biol. 10:1882.
  4. Liu, J. et al. (1994) Cytokine 6:272.
  5. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
  6. Grant, S.L. et al. (2001) Growth Factors 19:153.
  7. Chollangi, S. et al. (2012) J. Biol. Chem. 287:32848.
  8. Larrea, E. et al. (2014) J. Hepatol. 60:482.

Alternate Names

MGC20461, oncostatin M, oncostatin-M

Entrez Gene IDs

5008 (Human); 18413 (Mouse)

Gene Symbol

OSM

UniProt

P13725

Additional Oncostatin M/OSM Products

Product Documents for Recombinant Human Oncostatin M (HEK-293-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8475-OM (with carrier)
Download SDS 8475-OM/CF (carrier free)

Product Specific Notices for Recombinant Human Oncostatin M (HEK-293-expressed) Protein

For research use only

Citations for Recombinant Human Oncostatin M (HEK-293-expressed) Protein

Customer Reviews for Recombinant Human Oncostatin M (HEK-293-expressed) Protein (1)

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FAQs for Recombinant Human Oncostatin M (HEK-293-expressed) Protein

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    • Q: Is Recombinant Human Oncostatin M (HEK-293-expressed) Protein (Catalog # 8475-OM) considered the pre-OSM peptide or mature protein?
      A: Recombinant Human Oncostatin M (HEK-293-expressed) Protein (Catalog # 8475-OM) is comprised of amino acids Ala26-Arg221 of Accession # P13725. This region is the 196 residue mature form of OSM.

    • Q: What are the differences between Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM)?
      A: Recombinant Human Oncostatin M Protein (Catalog # 295-OM) and Recombinant Human Oncostatin M (HEK293-expressed) Protein (Catalog # 8475-OM) share the same protein sequence, Ala26-Arg221 of Accession # P13725, but Catalog # 295-OM was expressed in E. coli and Catalog # 8475-OM was expressed in HEK293 cells. They are offered in formulations with or without BSA as a carrier protein. Both proteins are routinely tested and have the same activity range in our established QC bioassay: "Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells." The ED50 for this effect is 0.05-0.3 ng/mL. Catalog # 8475-OM is produced in a mammalian expression system so we would expect it to be glycosylated. Catalog # 295-OM is produced in E coli and is not glycosylated. If endotoxin levels are of concern for your application, we would recommend selecting the Catalog # 295-OM as it has an endotoxin threshold of <0.01 EU per 1 μg of protein. Catalog # 8475-OM has a higher endotoxin threshold of <0.10 EU per 1 μg of the protein.  That said, if it is important to use a mammalian expressed protein like Catalog # 8475-OM in your assay system, and you require endortoxin lower than <0.10 EU/1 μg of the protein please consult technical service to see if custom testing is an option. We also recommend reviewing the references under the Citations tab on the respective product pages to check the types of applications in which these two proteins have been evaluated.
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