Recombinant Human P-Cadherin Fc Chimera Protein, CF

R&D Systems | Catalog # 861-PC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human P-Cadherin Fc Chimera Protein (861-PC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

CAA45177

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived human P-Cadherin protein
Human P-Cadherin (Asp108 - Gly654)
Accession # CAA45177		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp108

Predicted Molecular Mass

87.4 kDa

SDS-PAGE

120-130 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.
When 1 x 105 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 30-60% will adhere after 1.5 hours at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.

Reviewed Applications

Read 2 reviews rated 4.5 using 861-PC in the following applications:

Formulation, Preparation, and Storage

861-PC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.

References

  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.

Long Name

Placental Cadherin

Alternate Names

CAD3, Cadherin-3, CDH3, CDHP, HJMD, PCAD, PCadherin

Entrez Gene IDs

1001 (Human); 12560 (Mouse); 116777 (Rat)

Gene Symbol

CDH3

UniProt

CAA45177

Additional P-Cadherin Products

Product Documents for Recombinant Human P-Cadherin Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human P-Cadherin Fc Chimera Protein, CF

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Recombinant Human P-Cadherin Fc Chimera Protein, CF

Customer Reviews for Recombinant Human P-Cadherin Fc Chimera Protein, CF (2)

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  • Recombinant Human P-Cadherin Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/30/2025
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    Name: Tan Trinh
    Application: In vivo study
    Verified Customer | Posted 07/25/2016

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