Recombinant Human P-Cadherin Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
861-PC-100
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human P-Cadherin Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1 x 105 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 30-60% will adhere after 1.5 hours at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Mouse myeloma cell line, NS0-derived human P-Cadherin protein
Human P-Cadherin (Asp108 - Gly654)
Accession # CAA45177
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp108
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
87.4 kDa
SDS-PAGE
120-130 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

861-PC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Entrez Gene IDs
1001 (Human); 12560 (Mouse); 116777 (Rat)
Alternate Names
CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin

Citations for Recombinant Human P-Cadherin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cadherin engagement improves insulin secretion of single human beta-cells.
    Authors: Parnaud G, Lavallard V, Bedat B, Matthey-Doret D, Morel P, Berney T, Bosco D
    Diabetes, 2015;64(3):887-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  2. Cadherin 6 has a functional role in platelet aggregation and thrombus formation.
    Authors: Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D
    Arterioscler. Thromb. Vasc. Biol., 2012;32(7):1724-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human P-Cadherin Fc Chimera Protein, CF
By Anonymous on 07/25/2016
Application: In vivo study