Recombinant Human PANP Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
7920-PN-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human PANP Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED50 for this effect is 25-100 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human PANP protein
Human PANP
(Ser76-Pro178)
Accession # Q8IYJ0
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser76 & Val80
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37 kDa (monomer)
SDS-PAGE
58-69 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7920-PN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PANP

PILR‑alpha associated neural protein (PANP) is an approximately 30 kDa type 1 transmembrane glycoprotein that contains a 147 amino acid (aa) extracellular domain (ECD) and an 83 aa cytoplasmic domain (1). Within the ECD, human PANP shares 93% aa sequence identity with mouse and rat PANP. Alternative splicing may generate an additional isoform with a 6 aa truncation at the C‑terminus. PANP is preferentially expressed in the central nervous system (1). It binds to PILR‑alpha, an inhibitory receptor that is expressed on dendritic cells, neutrophils, and macrophages (1, 2). PILR‑alpha additionally binds to CD99, NPDC‑1, CL‑P1/COLEC12, and glycoprotein B of Herpes simplex virus type 1 (3‑5). PILR‑alpha binding requires the presence of sialylated O‑linked carbohydrates on each of these ligands, including PANP (1, 3, 4, 6). The in cis association of PILR‑alpha with sialylated molecules on the neutrophil surface inhibits beta integrin activation and neutrophil extravasation during inflammatory responses (7).

References
  1. Kogure, A. et al. (2011) Biochem. Biophys. Res. Commun. 405:428.
  2. Fournier, N. et al. (2000) J. Immunol. 165:1197.
  3. Wang, J. et al. (2008) J. Immunol. 180:1686.
  4. Sun, Y. et al. (2012) J. Biol. Chem. 287:15837.
  5. Satoh, T. et al. (2008) Cell 132:935.
  6. Wang, J. et al. (2009) J. Virol. 83:13042.
  7. Wang, J. et al. (2013) Nat. Immunol. 14:34.
Long Name
PILR alpha Associated Neural Protein
Entrez Gene IDs
196500 (Human); 319352 (Mouse); 312711 (Rat)
Alternate Names
C12orf53; chromosome 12 open reading frame 53; PANP; PIANP; PILR alpha associated neural protein

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