Recombinant Human Parkin pS65 Protein, CF

Catalog # Availability Size / Price Qty
E3-166-025
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
FAQs
Reviews

Recombinant Human Parkin pS65 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application. Unlike unphosphorylated Parkin (E3-160), Parkin pS65 has substantial ligase activity in the absence of added activators as determined by its ability to autoubiquitinate, and ubiquitinate a Miro/Rhot1 substrate (SP-490).

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Parkin protein
Accession #
Predicted Molecular Mass
52 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-166

Formulation X mg/ml (X μM) in 25 mM Tris pH 8.5, 200 mM NaCl, 0.03% Brij35, 10% (v/v) Glycerol,  5 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Parkin

The E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene) is an essential part of the cellular machinery that participates in the removal of damaged mitochondria. Mutations in PARK2 are known to cause a form of Parkinson's disease known as autosomal recessive juvenile Parkinson's disease (AR-JP), and the mechanisms by which defective Parkin ligase contributes to the dopaminergic cell death in this disease is an area of intense investigation.

Reported substrates for Parkin include BCL2, GPR37, MIRO1, MFN1, MFN2, TOMM20, USP30, and many others. Parkin (an RBR-class Ubiquitin ligase) structures have recently been reported by multiple groups, and reveal that the ligase is folded upon itself to produce an auto-inhibited state. The auto-inhibition is relieved by interactions with PINK1 kinase (which can phosphorylate both Parkin and Ubiquitin at serine residue number 65) and pS65 phospho-Ubiquitin by mechanisms that are under investigation.

In vitro, Parkin may be activated by treatment with recombinant PINK1, or addition of low concentrations of pS65-phosphoubiquitin. Parkin has been reported to generate poly-Ubiquitin chains in K6, K11, K48, and K63 linkages both in vitro and in vivo. This recombinant protein is phosphorylated on serine 65, and is untagged.

References
  1. Bingol, B. et al. (2014) Nature 510: 370
  2. Ordureau, A. et al. (2014) Mol. Cell 56: 360
  3. Riley, B.E. et al. (2013) Nat. Comm. 4: doi:10.1038/ncomms2982
  4. Saraff, S.A. et al. (2013) Nature 496: 372
  5. Spratt, D.E. et al. (2013) Nat. Comm. 4: doi:10.1038/ncomms2983
  6. Trempe, J.F. et al. (2013) Science 340: 1451
  7. Wauer T. et. al. (2015) Nature 524: 370
  8. Wauer T. & Komander, D. (2013) EMBO J 32: 2099
Long Name
Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1
Entrez Gene IDs
5071 (Human); 50873 (Mouse); 56816 (Rat)
Alternate Names
AR-JP; E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; EC 6.3.2.-; LPRS2; PARK2; parkin 2; Parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PDJjuvenile) 2, parkin; PRKN

Citation for Recombinant Human Parkin pS65 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy
    Authors: ND Roverato, C Sailer, N Catone, A Aichem, F Stengel, M Groettrup
    Cell Reports, 2021;34(11):108857.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Parkin pS65 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Parkin pS65 Protein, CF and earn rewards!

Have you used Recombinant Human Parkin pS65 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review