Recombinant Human PDGFRL Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1723-PR-050
Recombinant Human PDGFRL Fc Chimera Protein, CF Bioactivity
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Recombinant Human PDGFRL Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of HCT-116 human colorectal carcinoma cells. The ED50 for this effect is 1.5-9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human PDGFRL protein
Human PDGFRL
(Gln22-Ser375)
Accession # Q15198
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gln22 inferred from enzymatic pyroglutamate treatment revealing His23.
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
68 kDa
SDS-PAGE
69-84 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1723-PR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human PDGFRL Fc Chimera (Catalog # 1723-PR) inhibits HCT-116 human Colorectal carcinoma proliferation. The ED50 for this effect is 1.5-9 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human PDGFRL Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 69- 84 kDa and 140- 170 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGFRL

Platelet-derived growth factor receptor-like protein is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, human PDGFRL is 90% similar to the mouse version and 91% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). PDGFRL is a tumor suppressor active in the tumor-suppression network and implicated in colorectal cancer, and a decrease in PDGFRL expression levels has been observed in breast cancer (1). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (6). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (7). Study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation.

References
  1. Guo, F-J. et al. (2010) World J Gastroenterol. 16:1465.
  2. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  3. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  4. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
  5. Matsui, T. et al. (1989) Science 243:800.
  6. Hou, S. et al. (2013). Human Mutat. 34:74.
  7. Fujikado, N. et al. (2006). Arthritis Res. & Ther. 8:R100.
  8. Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.
Long Name
Platelet-derived Growth Factor Receptor-like
Entrez Gene IDs
5157 (Human); 68797 (Mouse); 290771 (Rat)
Alternate Names
PDGFRL; PDGFR-Like Protein; PDGRL; platelet-derived growth factor receptor-like protein; platelet-derived growth factor receptor-like; platelet-derived growth factor-beta-like tumor suppressor; PRLTS; PRLTSPDGF receptor beta-like tumor suppressor

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