Recombinant Human Pepsinogen C/PGC Protein, CF

R&D Systems | Catalog # 6186-AS

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Pepsinogen C/PGC Protein (6186-AS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P20142

Applications

Enzyme Activity
View all Pepsinogen C/PGC Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Pepsinogen C/PGC protein
Met1-Ala388, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala17 & Leu43

Predicted Molecular Mass

41 kDa & 38 kDa

SDS-PAGE

35-40 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >700 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

6186-AS
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Pepsinogen C/PGC

Pepsinogen C (progastricsin) is an aspartic proteinase zymogen that belongs to the peptidase family A1. Pespsin C, the activated protease, is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is synthesized as an inactive zymogen that is converted into its active mature form at low pH by sequential cleavage of the prosegment in an autocatalytic process (1). Polymorphisms in this gene are associated with susceptibility to gastric cancers (2). Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis (3).

References

  1. Richter, C. et al. (1998) Biochem. J. 335:481.
  2. Liu, H. J. et al. (2003) World J. Gastroenterol. 9:50.
  3. Plebani, M. (1993) Crit. Rev. Clin. Lab. Sci. 30:273.

Alternate Names

Gastricsin, PEPC, PGC, PGII

UniProt

P20142

Additional Pepsinogen C/PGC Products

Product Documents for Recombinant Human Pepsinogen C/PGC Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Pepsinogen C/PGC Protein, CF

For research use only

Related Research Areas

Aspartic Proteases and Regulators

Citations for Recombinant Human Pepsinogen C/PGC Protein, CF

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Protocols

View specific protocols for Recombinant Human Pepsinogen C/PGC Protein, CF (6186-AS):

Materials
  • Assay Buffer: 50 mM Sodium Citrate, 250 mM NaCl, pH 2.5
  • Recombinant Human Pepsinogen C/PGC (rhPGC) (Catalog # 6186-AS)
  • Substrate: MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH (Catalog # ES005), 2 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhPGC to 100 µg/mL in Assay Buffer.
  2. Incubate 100 µg/mL rhPGC for 10 minutes at room temperature.
  3. Dilute 100 µg/mL rhPGC to 0.5 µg/mL in Assay Buffer.
  4. Dilute Substrate to 20 µM in Assay Buffer.
  5. Load 50 µL of 0.5 µg/mL of rhPGC into a plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 20 µM Substrate.
  6. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:

  • rhPGC: 0.025 µg
  • Substrate: 10 µM

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