Recombinant Human PGLYRP1/PGRP-S Protein, CF

Catalog # Availability Size / Price Qty
2590-PGB-050
Bioactivity graph of recombinant human PGLYRP1 / PGRP-S Protein
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Product Details
Citations (1)
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Recombinant Human PGLYRP1/PGRP-S Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PGLYRP1/PGRP-S that produces 50% optimal binding response is 0.75-4.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human PGLYRP1/PGRP-S protein
Gln22-Pro196, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
20 kDa
SDS-PAGE
23-27 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2590-PGB

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Recombinant Human PGLYRP1/PGRP-S protein View Larger

When peptidoglycan is coated at 1 µg/mL, 100 µL/well, Recombinant Human PGLYRP1/PGRP-S binds with an ED50 of 0.75-4.5 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PGLYRP1/PGRP-S

The human PGRP family is comprised of four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP-L, PGRP-I alpha, PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 576 amino acids (aa) in length, while PGRP-I alpha and I beta are (I) intermediate in length at 341 aa and 373 aa, respectively, and PGRP-S is the shortest at 196 aa in length (3, 4). All human PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C-terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Human PGRP-S, the first described member of the family, is a 28 kDa secreted glycoprotein associated with neutrophils (4). The mature molecule is 175 aa in length and contains three variably-sized peptide-carbohydrate recognition sequences of 15 aa, 29 aa and 49 aa, respectively. Human PGRP-S is 72%, 71% and 70% aa identical to mouse, bovine and rat mature PGRP-S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with non-pathogenic bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The longer three PGRP members are all membrane-bound molecules that contain two membrane-spanning segments. Both the N- and C-termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C-terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).

References
  1. Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777.
  2. Steiner, H. (2004) Immunol. Rev. 198:83.
  3. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  4. Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078.
  5. Dziarski, R. et al. (2003) Blood 102:689.
Long Name
Peptidoglycan Recognition Protein Short/Peptidoglycan Recognition Protein 1
Entrez Gene IDs
8993 (Human); 21946 (Mouse)
Alternate Names
peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; PGLYRP; PGLYRP1; PGRPpeptidoglycan recognition protein; PGRPS; PGRP-S; PGRP-SMGC126894; PGRPSMGC126896; TAG7; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein); TNFSF3L

Citation for Recombinant Human PGLYRP1/PGRP-S Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cutting Edge: identification of neutrophil PGLYRP1 as a ligand for TREM-1.
    Authors: Read C, Kuijper J, Hjorth S, Heipel M, Tang X, Fleetwood A, Dantzler J, Grell S, Kastrup J, Wang C, Brandt C, Hansen A, Wagtmann N, Xu W, Stennicke V
    J Immunol, 2015;194(4):1417-21.
    Species: Human
    Sample Types:
    Applications: Surface Plasmon Resonance

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