Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF

R&D Systems | Catalog # 8507-PG

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human PGLYRP3/PGRP-I alpha Protein (8507-PG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q96LB9

Structure / Form

Disulfide-linked Homodimer

Applications

Binding Activity
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Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human PGLYRP3/PGRP-I alpha protein
Trp18-His341, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp18

Predicted Molecular Mass

37 kDa

SDS-PAGE

34-40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP3/PGRP-I alpha that produces 50% optimal binding response is 10-60 ng/mL.

Formulation, Preparation, and Storage

8507-PG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PGLYRP3/PGRP-I alpha

PGLYRP3, also known as PGRP-1 alpha, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP3 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP3 contains two nonidentical PGRP domains, and it shares 74% and 75% amino acid sequence identity with mouse and rat PGLYRP3, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP3 is directly cytotoxic against pathogenic and nonpathogenic Gram-positive and Gram-negative bacteria (3, 5). Its bactericidal activity requires physiological concentrations of Zn2+ (5). PGLYRP3 has also been shown to have an anti-inflammatory role in intestinal epithelial cells (6). PGLYRP3 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (7-9). In humans, PGLYRP3 single nucleotide polymorphisms have been associated with inflammatory bowel disease (10).

References

  1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  2. Park, S.Y. et al. (2011) PLoS One 6:e24961.
  3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
  4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
  5. Wang, M. et al. (2007) J. Immunol. 178:3116.
  6. Zenhom, M. et al. (2012) Immunobiology 217:412.
  7. Park, S.Y. et al. (2011) PLoS One 6:e24961.
  8. Saha, S. et al. (2010) Cell Host Microbe 8:147.
  9. Jing, X. et al. (2014) J. Immunol. 193:3055.
  10. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.

Long Name

Peptidoglycan Recognition Protein Intermediate, Alpha/Peptidoglycan Recognition Protein 3

Alternate Names

PGRP-I alpha, PGRP1A, PGRPI alpha, PGRPIA

Entrez Gene IDs

114771 (Human); 242100 (Mouse); 499658 (Rat)

Gene Symbol

PGLYRP3

UniProt

Q96LB9

Additional PGLYRP3/PGRP-I alpha Products

Product Documents for Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF

For research use only

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