Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF

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Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP3/PGRP-I alpha that produces 50% optimal binding response is 10-60 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human PGLYRP3/PGRP-I alpha protein
Trp18-His341, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked Homodimer
Predicted Molecular Mass
37 kDa
34-40 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: PGLYRP3/PGRP-I alpha

PGLYRP3, also known as PGRP-1 alpha, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP3 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP3 contains two nonidentical PGRP domains, and it shares 74% and 75% amino acid sequence identity with mouse and rat PGLYRP3, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP3 is directly cytotoxic against pathogenic and nonpathogenic Gram-positive and Gram-negative bacteria (3, 5). Its bactericidal activity requires physiological concentrations of Zn2+ (5). PGLYRP3 has also been shown to have an anti-inflammatory role in intestinal epithelial cells (6). PGLYRP3 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (7-9). In humans, PGLYRP3 single nucleotide polymorphisms have been associated with inflammatory bowel disease (10).

  1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  2. Park, S.Y. et al. (2011) PLoS One 6:e24961.
  3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
  4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
  5. Wang, M. et al. (2007) J. Immunol. 178:3116.
  6. Zenhom, M. et al. (2012) Immunobiology 217:412.
  7. Park, S.Y. et al. (2011) PLoS One 6:e24961.
  8. Saha, S. et al. (2010) Cell Host Microbe 8:147.
  9. Jing, X. et al. (2014) J. Immunol. 193:3055.
  10. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.
Long Name
Peptidoglycan Recognition Protein Intermediate, Alpha/Peptidoglycan Recognition Protein 3
Entrez Gene IDs
114771 (Human); 242100 (Mouse); 499658 (Rat)
Alternate Names
peptidoglycan recognition protein 3; peptidoglycan recognition protein I alpha; Peptidoglycan recognition protein I-alpha; Peptidoglycan recognition protein intermediate alpha; peptidoglycan recognition protein-I-alpha; PGLYRP3; PGLYRPIalphaMGC149197; PGRP1A; PGRPI alpha; PGRP-I alpha; PGRPIA; PGRP-Ialpha; PGRPIAPGRP-I-alpha


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