Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

Catalog # Availability Size / Price Qty
8389-PG-050
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Recombinant Human PGLYRP4/PGRP-I beta Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP4/PGRP-I beta that produces 50% optimal binding response is 0.5-3 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human PGLYRP4/PGRP-I beta protein
Asp18-His373 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa
SDS-PAGE
53-64 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8389-PG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PGLYRP4/PGRP-I beta

PGLYRP4, also known as PGRP-I beta, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP4 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP4 contains two nonidentical PGRP domains, and it shares 77% and 74% amino acid sequence identity with mouse and rat PGLYRP4, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP4 is directly bactericidal against pathogenic and nonpathogenic Gram-positive bacteria, but not normal flora bacteria, suggesting that normal flora bacteria have developed resistance to this bactericidal mechanism (3, 5, 6). Its bactericidal activity requires physiological concentrations of Zn2+ (6). PGLYRP4 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (2, 7). In humans, PGLYRP4 single nucleotide polymorphisms have been associated with inflammatory bowel disease and increased Parkinson’s disease risk (8, 9).

References
  1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  2. Park, S.Y. et al. (2011) PLoS One 6:e24961.
  3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
  4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
  5. Tydell, C.C. et al. (2006) J. Immunol. 176:1154.
  6. Wang, M. et al. (2007) J. Immunol. 178:3116.
  7. Saha, S. et al. (2010) Cell Host Microbe 8:147.
  8. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.
  9. Goldman, S.M. et al. (2014) Mov. Disord. 29:1171.
Long Name
Peptidoglycan Recognition Protein Intermediate, Beta/Peptidoglycan Recognition Protein 4
Entrez Gene IDs
57115 (Human)
Alternate Names
peptidoglycan recognition protein 4; peptidoglycan recognition protein I beta; Pglyrp-1 beta; PGLYRP4; PGRPI beta; PGRP-I beta; PGRP-I-beta

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