Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

R&D Systems | Catalog # 8389-PG

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human PGLYRP4/PGRP-I beta Protein (8389-PG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

AAK72485

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all PGLYRP4/PGRP-I beta Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PGLYRP4/PGRP-I beta protein
Asp18-His373 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

40 kDa

SDS-PAGE

53-64 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP4/PGRP-I beta that produces 50% optimal binding response is 0.5-3 μg/mL.

Formulation, Preparation, and Storage

8389-PG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PGLYRP4/PGRP-I beta

PGLYRP4, also known as PGRP-I beta, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP4 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP4 contains two nonidentical PGRP domains, and it shares 77% and 74% amino acid sequence identity with mouse and rat PGLYRP4, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP4 is directly bactericidal against pathogenic and nonpathogenic Gram-positive bacteria, but not normal flora bacteria, suggesting that normal flora bacteria have developed resistance to this bactericidal mechanism (3, 5, 6). Its bactericidal activity requires physiological concentrations of Zn2+ (6). PGLYRP4 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (2, 7). In humans, PGLYRP4 single nucleotide polymorphisms have been associated with inflammatory bowel disease and increased Parkinson’s disease risk (8, 9).

References

  1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  2. Park, S.Y. et al. (2011) PLoS One 6:e24961.
  3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
  4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
  5. Tydell, C.C. et al. (2006) J. Immunol. 176:1154.
  6. Wang, M. et al. (2007) J. Immunol. 178:3116.
  7. Saha, S. et al. (2010) Cell Host Microbe 8:147.
  8. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.
  9. Goldman, S.M. et al. (2014) Mov. Disord. 29:1171.

Long Name

Peptidoglycan Recognition Protein Intermediate, Beta/Peptidoglycan Recognition Protein 4

Alternate Names

Pglyrp-1 beta, PGRP-I beta, PGRPI beta

Entrez Gene IDs

57115 (Human)

Gene Symbol

PGLYRP4

UniProt

AAK72485

Additional PGLYRP4/PGRP-I beta Products

Product Documents for Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

For research use only

Related Research Areas

Host Defense/Innate Immunity

Customer Reviews for Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human PGLYRP4/PGRP-I beta Protein, CF and earn rewards!

Have you used Recombinant Human PGLYRP4/PGRP-I beta Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes