Recombinant Human PILR-beta His-tag Protein, CF

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Product Details

Recombinant Human PILR-beta His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human CL-P1/COLEC12  (Catalog # 2690-CL) is immobilized at 2 μg/mL (100 μL/well), it binds Recombinant Human PILR‑ beta (Catalog # 10319-PR) with an ED50 of 1-10 μg/mL
Human embryonic kidney cell, HEK293-derived human PILR-beta protein
Gln20-Ala189, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Gln20, as confirmed by mass spectrometry
Predicted Molecular Mass
20 kDa
30-40 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant Human PILR-beta His-tag Protein (Catalog # 10319-PR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 30-40 kDa under reducing and non-reducing conditions.

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Background: PILR-beta

Paired immunoglobulin-like type 2 receptor beta (PILR-beta ) is a type I transmembrane (TM) glycoprotein that belongs to the Ig superfamily (1). PILR-beta is the activating counterpart to the inhibitory receptor immunoreceptor PILR-alpha which contains a tyrosine-based inhibitory motif (ITIM) (1). Mature human PILR-beta contains a V‑type Ig‑like extracellular domain (ECD) with a siglec-like fold, a single TM domain, and a truncated cytoplasmic tail (2, 3). The TM domain of PILR-beta contains a positively‑charged residue which interacts with immunoreceptor tyrosine-based activation (ITAM)-bearing adaptor molecules (2). The ECD of mature human PILR-beta shares 40% amino acid sequence identity with its mouse counterpart. PILR-beta is expressed on myeloid cells, such as natural killer, macrophage, and dendritic cells, as well as resident cells of the central nervous system, including microglial cells (2, 4). It is a binding partner for DAP12 and CD99 and has been shown to play an important role in innate immunity and inflammation (4-6). The PILR-alpha / beta pair have also been shown to regulate cell signaling via association with SHP-1 (7). Experiments studying the effects of S. aureus and T. gondii infections in mice have shown that up-regulation of PILR-beta led to significantly lower survival rates while knock-down of PILR-beta or activation of PILR-alpha resulted in significantly less inflammation and increased pathogen clearance (4, 5).

  1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
  2. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
  3. Lu, Q. et al. (2014) PNAS 111:8221.
  4. Tato, C.M. et al. (2012) PLoS One 7:e31690.
  5. Banerjee, A. et al. (2010) Infect. Immun. 78:1353.
  6. Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.
  7. Mousseau, D.D. et al. (2000) J. Biol. Chem. 275:4467.
Long Name
Paired-Ig-likeType 2 Receptor beta
Entrez Gene IDs
29990 (Human); 170741 (Mouse)
Alternate Names
activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta


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