Recombinant Human Plexin A4 Protein, CF

Catalog # Availability Size / Price Qty
5856-PA-050

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Recombinant Human Plexin A4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin A4 is immobilized at 5 μg/mL, Recombinant Human Semaphorin 6A Fc Chimera (Catalog # 1146-S6) binds with an apparent Kd <20 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Plexin A4 protein
Thr24-Pro1237, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr24
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
135.9 kDa (monomer)
SDS-PAGE
140-150 kDa, reducing conditions

Product Datasheets

You must select a language.

x

5856-PA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5856-PA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Plexin A4

Plexin A4 is a 220‑230 kDa member of the plexin A subfamily, plexin family of proteins (1). It is found on sensory, autonomic and motor neurons and oligodendrocytes, plus T cells and dendritic cells (1‑8). Mature human Plexin A4 is an 1871 amino acid (aa) type I transmembrane glycoprotein with a 23 aa signal sequence, a 1214 aa extracellular domain (ECD), and a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 51‑482), three PSI domains (aa 509‑856) and four IPT regions (aa 858‑1230) that contain a phosphoserine at aa 946 (1). Of three isoform variants, one shows a 65 aa substitution for aa 458‑1894, a second shows an 80 aa substitution for aa 1292‑1894, and a third shows the just mentioned 80 aa substitution coupled to a 14 aa substitution for aa 1‑535 (9). The human Plexin A4 ECD shares 97% aa identity with mouse, equine, canine, and bovine Plexin A4. Full‑length Plexin A4 also shares 67% aa identity with the most related family member, Plexin A2. Plexin A4 regulates cell migration, activation and axon guidance via repulsion (1‑5). It serves as a receptor for transmembrane semaphorins, Sema6A and 6B, and as a coreceptor with neuropilin-1 for the secreted semaphorin, Sema3A (1‑8). During development, it plays a role in nerve migration and midline crossing and down‑regulates dendrite formation (2‑8). It is often co‑expressed with Plexin A3, which can also engage class 6 semaphorins but prefers Sema3F/ neuropilin‑2 to Sema3A/neuropilin-1 (3, 8). Thus, Plexins A3 and A4 are redundant in some functions, but unique in others. In T cells, Plexin A4 engages Sema3A and negatively regulates TCR signals (6).

References
  1. Suto, F. et al. (2003) Mech. Dev. 120:385.
  2. Suto, F. et al. (2005) J. Neurosci. 25:3628.
  3. Faulkner, R.L. et al. (2008) Neural Dev. 3:21.
  4. Waimey, K.E. et al. (2008) Dev. Biol. 315:448.
  5. Runker, A.E. et al. (2008) Neural Dev. 3:34.
  6. Yamamoto, M. et al. (2008) Int. Immunol. 20:413.
  7. Okada, A. et al. (2007) Biochem. Biophys. Res. Commun. 352:158.
  8. Yaron, A. et al. (2005) Neuron 45:513.
  9. Protein Accession # NP_861440, EAW83796, EAL24077.
Entrez Gene IDs
91584 (Human); 243743 (Mouse); 312213 (Rat)
Alternate Names
A; DKFZp434G0625; FAYV2820; FLJ35026; FLJ38287; KIAA1550DKFZp566O0546; PLEXA4; Plexin A4; plexin A4, B; plexin-A4; PLXNA4; PLXNA4A; PLXNA4B; PRO34003

Citation for Recombinant Human Plexin A4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Plexin-A4 mediates amyloid-&beta-induced tau pathology in Alzheimer's disease animal model
    Authors: S Chung, J Yang, HJ Kim, EM Hwang, W Lee, K Suh, H Choi, I Mook-Jung
    Progress in neurobiology, 2021;0(0):102075.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Recombinant Proteins

Reconstitution Buffers

Reviews for Recombinant Human Plexin A4 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Plexin A4 Protein, CF and earn rewards!

Have you used Recombinant Human Plexin A4 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review