Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

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Citations (6)
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Recombinant Human Semaphorin 6A Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED50 for this effect is 0.4-2.4 μg/mL.
Mouse myeloma cell line, NS0-derived human Semaphorin 6A protein
Human Semaphorin 6A
Accession # Q9H2E6
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
97 kDa (monomer)
120 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Semaphorin 6A

Semaphorin 6A (Sema6A) is an approximately 120 kDa member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, dendritic spine formation, and angiogenesis (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain a characteristic extracellular beta  propeller N-terminal semaphorin (sema) domain and also an extracellular
plexin-semaphorin-integrin (PSI) domain (1, 2). Within the ECD, human Sema6A shares 94% and 93% aa sequence identity with mouse and rat Sema6A, respectively. Alternative splicing generates an additional isoform with a 17 aa insertion between the sema domain and transmembrane segment. Sema6A interacts with Plexin A4
(3-7) to induce growth cone collapse and regulate the axon pathfinding of sympathetic neurons (3, 7, 8), cerebellar cortex granule cells (9), hippocampus CA3 region mossy fibers (6), corticospinal tract axons (5, 10), and retinal neurons (4). Plexin A2 can compete with Plexin A4 for Sema6A binding and thereby limit axon repulsion (6). In addition, Sema6A and Plexin A4 are co-expressed on dorsal root ganglion sensory neurons and associate in cis; this prevents in trans interactions and subsequent axon repulsion (7). Sema6A is additionally expressed on vascular endothelial cells where it regulates VEGF R2 responsiveness during angiogenesis (11). In contrast, exogenous Sema6A can inhibit the survival of the HUVEC cell line in a Plexin A4 independent manner (11, 12). Sema6A is also expressed on myelinating oligodendrocytes, Langerhans cells, and some dendritic cells (13, 14).

  1. Jongbloets, B.C. and R.J. Pasterkamp (2014) Development 141:3292.
  2. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
  3. Suto, F. et al. (2005) J. Neurosci. 25:3628.
  4. Matsuoka, R.L. et al. (2011) Nature 470:259.
  5. Runker, A.E. et al. (2008) Neural Dev. 3:34.
  6. Suto, F. et al. (2007) Neuron 53:535.
  7. Haklai-Topper, L. et al. (2010) EMBO J. 29:2635.
  8. Xu, X.M. et al. (2000) J. Neurosci. 20:2638.
  9. Kerjan, G. et al. (2005) Nat. Neurosci. 8:1516.
  10. Mauti, O. et al. (2007) Neural Dev. 2:28.
  11. Segarra, M. et al. (2012) Blood 120:4104.
  12. Kigel, B. et al. (2011) Blood 118:4285.
  13. Bernard, F. et al. (2012) Glia 60:1590.
  14. Gautier, G. et al. (2006) Am. J. Pathol. 168:453.
Entrez Gene IDs
57556 (Human); 20358 (Mouse)
Alternate Names
KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA

Citations for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants
    Authors: S Tian, X Xiong, J Zeng, S Wang, BJ Tremblay, P Chen, B Chen, M Liu, P Chen, K Sheng, D Zeve, W Qi, DT Breault, C Rodríguez, R Gerhard, R Jin, AC Doxey, M Dong
    Nature Communications, 2022;13(1):6786.
    Species: Human
    Sample Types: Protein
    Applications: Reference Standard
  2. Neurotrophin Promotes Neurite Outgrowth by Inhibiting Rif GTPase Activation Downstream of MAPKs and PI3K Signaling
    Authors: X Tian, H Yan, J Li, S Wu, J Wang, L Fan
    Int J Mol Sci, 2017;18(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Sema6a and Plxna2 mediate spatially regulated repulsion within the developing eye to promote eye vesicle cohesion.
    Authors: Ebert A, Childs S, Hehr C, Cechmanek P, McFarlane S
    Development, 2014;141(12):2473-82.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Semaphorin 6A regulates angiogenesis by modulating VEGF signaling.
    Authors: Segarra, Marta, Ohnuki, Hidetaka, Maric, Dragan, Salvucci, Ombretta, Hou, Xu, Kumar, Anil, Li, Xuri, Tosato, Giovanna
    Blood, 2012;120(19):4104-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Measles virus modulates dendritic cell/T-cell communication at the level of plexinA1/neuropilin-1 recruitment and activity.
    Authors: Tran-Van H, Avota E, Bortlein C, Mueller N, Schneider-Schaulies S
    Eur. J. Immunol., 2011;41(1):151-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells.
    Authors: Renaud J, Kerjan G, Sumita I, Zagar Y, Georget V, Kim D, Fouquet C, Suda K, Sanbo M, Suto F, Ackerman SL, Mitchell KJ, Fujisawa H, Chedotal A
    Nat. Neurosci., 2008;11(4):440-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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