Recombinant Human PRDC/GREM2 (Catalog # 8436-PR) inhibits BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.03-0.12 μg/mL
PRDC/GREM2 (Protein Related to DAN and Cerberus/Gremlin-2) is a secreted BMP antagonist belonging to the Cerberus/DAN (CAN) family. Similar to other CAN family members, PRDC/GREM2 has 6 conserved cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot (1-3). Mature human PRDC/GREM2 is synthesized as a 168 amino acid (aa) monomer. At the amino acid level, mature human PRDC/GREM2 shares 97% sequence identity with mature mouse PRDC/GREM2. PRDC/GREM2 is biologically active as a homodimer that assembles its subunits in a head-to-tail orientation. PRDC/GREM2 is up-regulated through Wnt/ beta -catenin signaling and has been shown to potently inhibit BMP-2, -4, and -7 (4-6). During early development PRDC/GREM2 is involved in tooth morphogenesis, osteogenesis, and neurogenesis (7-9). In the adult, PRDC/GREM2 is involved in bone repair and bone remodeling (10). PRDC/GREM2 has been detected in the adult ovary, brain, spleen, and colon (11). The biological activity of PRDC/GREM2 can be blocked by binding to heparin (4).
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Protein Related to DAN and Cerberus/Gremlin-2
Entrez Gene IDs:
64388 (Human); 23893 (Mouse)
CKTSF1B2; CKTSF1B2FLJ21195; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; DAND3; DAND3Prdc; GREM2; gremlin 2; gremlin 2, cysteine knot superfamily, homolog; gremlin-2; PRDC; PRDCgremlin 2, cysteine knot superfamily, homolog (Xenopus laevis); Protein related to DAN and cerberus
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