Recombinant Human PRL-3/PTP4A3 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Product Details

Recombinant Human PRL-3/PTP4A3 Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >0.4 pmol/min/μg, as measured under the described conditions.
E. coli-derived human PRL-3/PTP4A3 protein
Ala2-Met173, with N-terminal Met and 7-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
20 kDa
23 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 50 mM HEPES, 10 mM DTT, pH 7.5
  • Recombinant Human PRL-3/PTP4A3 (rhPTP4A3) (Catalog # 8455-PT)
  • Substrate: p-Nitrophenyl phosphate (Sigma, Catalog # N2765), 10 mM stock in deionized water
  • NaOH, 0.2 M in deionized water
  • 96-well Clear Plate (Catalog # DY990)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhPTP4A3 to 40 µg/mL in Assay buffer.
  2. Dilute Substrate to 5 mM in Assay buffer.
  3. Prepare reaction mixtures by combining equivalent volumes of dilute rhPTP4A3 and dilute Substrate in microtubes.  Include an Enzyme Control by combining dilute rhPTP4A3 with twice the volume of 0.2 M NaOH, mix briefly, then add a volume of dilute Substrate equivalent to the volume of rhPTP4A3. The Enzyme Control will have 2x the volume of the reaction mixture.
  4. Incubate Reactions and Enzyme Controls at 37 °C for 24 hours.
  5. Load 100 µL of Reactions into a plate in triplicate and stop the reactions by adding 100 µL 0.2 M NaOH. 
  6. Load 200 µL of Enzyme Controls into plate in triplicate.
  7. Read plate at 410 nm (absorbance) in endpoint mode.
  8. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)


      *Adjusted for Enzyme Controls.
      **Derived using calibration standard p-Nitrophenol (Sigma, Catalog # 241326).

Per Well:
  • rhPTP4A3: 2 µg
  • pNPP: 1.25 mM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: PRL-3/PTP4A3

Phosphatase of regenerating liver 3 (PRL-3), also known as protein-tyrosine phosphatase 4A3 (PTP4A3), is a member of the PRL subgroup of PTPases (1). It is preferentially expressed in skeletal muscle and the heart (2). Human PRL-3 shares 97% amino acid sequence identity with mouse and rat PRL-3. Structurally, PRL-3 consists of a five-stranded beta -sheet and six alpha -helices (3, 4). It has been shown to associate with membranes in a farnesylation-dependent manner (5). Both PRL-3 over-expression and attenuation of PRL-3 expression results in cell cycle arrest, suggesting that basal expression levels of this enzyme are important for normal cell cycle progression (6). PRL-3 has been shown to activate NF-kappa B signaling and may itself be regulated by FKBP38 (7, 8). PRL-3 also promotes epithelial to mesenchymal transition, tumor angiogenesis, cell migration, invasion, and metastasis, and it is over-expressed in multiple human cancers (9-18). Src-mediated phosphorylation of PRL-3 may be required for PRL-3-dependent cell migration and invasion (19).

  1. Bessette, D.C. et al. (2008) Cancer Metastasis Rev. 27:231.
  2. Zeng, Q. et al. (1998) Biochem. Biophys. Res. Commun. 244:421.
  3. Kozlov, G. et al. (2004) J. Biol. Chem. 279:11882.
  4. Kim, K.A. et al. (2004) FEBS Lett. 565:181.
  5. Zeng, Q. et al. (2000) J. Biol. Chem. 275:21444.
  6. Basak, S. et al. (2008) Mol. Cell 30:303.
  7. Lian, S. et al. (2013) Biochem. Biophys. Res. Commun. 430:196.
  8. Choi, M.S. et al. (2011) Biochem. Biophys. Res. Commun. 406:305.
  9. Wang, H. et al. (2007) Cancer Res. 67:2922.
  10. Liu, Y. et al. (2009) Cancer Biol. Ther. 8:1352.
  11. Pryczynicz, A. et al. (2014) Tumour Biol. 35:6587.
  12. Guo, K. et al. (2006) Cancer Res. 66:9625.
  13. Ming, J. et al. (2014) Pathobiology 81:1.
  14. Zeng, Q. et al. (2003) Cancer Res. 63:2716.
  15. Kato, H. et al. (2004) Clin. Cancer Res. 10:7318.
  16. Al-Aidaroos, A.Q. and Q. Zeng (2010) J. Cell. Biochem. 111:1087.
  17. Jiang, Y. et al. (2011) Cancer Res. 71:234.
  18. Krndija, D. et al. (2012) J. Cell Sci. 125:3883.
  19. Fiordalisi, J.J. et al. (2013) PLoS One 8:e64309.
Long Name
Phosphatase of Regenerating Liver 3
Entrez Gene IDs
11156 (Human); 19245 (Mouse)
Alternate Names
PRL3; PRL-3; PRL3EC; PRL-3potentially prenylated protein tyrosine phosphatase; PRL-Rprotein tyrosine phosphatase type IVA 3; protein tyrosine phosphatase type IVA, member 3; Protein-tyrosine phosphatase 4a3; Protein-tyrosine phosphatase of regenerating liver 3; PTP4A3


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