Phosphatase of regenerating liver 3 (PRL-3), also known as protein-tyrosine phosphatase 4A3 (PTP4A3), is a member of the PRL subgroup of PTPases (1). It is preferentially expressed in skeletal muscle and the heart (2). Human PRL-3 shares 97% amino acid sequence identity with mouse and rat PRL-3. Structurally, PRL-3 consists of a five-stranded beta -sheet and six alpha -helices (3, 4). It has been shown to associate with membranes in a farnesylation-dependent manner (5). Both PRL-3 over-expression and attenuation of PRL-3 expression results in cell cycle arrest, suggesting that basal expression levels of this enzyme are important for normal cell cycle progression (6). PRL-3 has been shown to activate NF-kappa B signaling and may itself be regulated by FKBP38 (7, 8). PRL-3 also promotes epithelial to mesenchymal transition, tumor angiogenesis, cell migration, invasion, and metastasis, and it is over-expressed in multiple human cancers (9-18). Src-mediated phosphorylation of PRL-3 may be required for PRL-3-dependent cell migration and invasion (19).
Recombinant Human PRL-3/PTP4A3 Protein, CF
R&D Systems | Catalog # 8455-PT
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human PRL-3/PTP4A3 Protein (8455-PT)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human PRL-3/PTP4A3 protein
Ala2-Met173, with N-terminal Met and 7-His tag
Ala2-Met173, with N-terminal Met and 7-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
20 kDa
SDS-PAGE
23 kDa, reducing conditions
Activity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >0.4 pmol/min/μg, as measured under the described conditions.
The specific activity is >0.4 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
8455-PT
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PRL-3/PTP4A3
References
- Bessette, D.C. et al. (2008) Cancer Metastasis Rev. 27:231.
- Zeng, Q. et al. (1998) Biochem. Biophys. Res. Commun. 244:421.
- Kozlov, G. et al. (2004) J. Biol. Chem. 279:11882.
- Kim, K.A. et al. (2004) FEBS Lett. 565:181.
- Zeng, Q. et al. (2000) J. Biol. Chem. 275:21444.
- Basak, S. et al. (2008) Mol. Cell 30:303.
- Lian, S. et al. (2013) Biochem. Biophys. Res. Commun. 430:196.
- Choi, M.S. et al. (2011) Biochem. Biophys. Res. Commun. 406:305.
- Wang, H. et al. (2007) Cancer Res. 67:2922.
- Liu, Y. et al. (2009) Cancer Biol. Ther. 8:1352.
- Pryczynicz, A. et al. (2014) Tumour Biol. 35:6587.
- Guo, K. et al. (2006) Cancer Res. 66:9625.
- Ming, J. et al. (2014) Pathobiology 81:1.
- Zeng, Q. et al. (2003) Cancer Res. 63:2716.
- Kato, H. et al. (2004) Clin. Cancer Res. 10:7318.
- Al-Aidaroos, A.Q. and Q. Zeng (2010) J. Cell. Biochem. 111:1087.
- Jiang, Y. et al. (2011) Cancer Res. 71:234.
- Krndija, D. et al. (2012) J. Cell Sci. 125:3883.
- Fiordalisi, J.J. et al. (2013) PLoS One 8:e64309.
Long Name
Phosphatase of Regenerating Liver 3
Alternate Names
PRL3, PTP4A3
Gene Symbol
PTP4A3
UniProt
Additional PRL-3/PTP4A3 Products
Product Documents for Recombinant Human PRL-3/PTP4A3 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human PRL-3/PTP4A3 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human PRL-3/PTP4A3 Protein, CF (8455-PT):
Materials
- Assay Buffer: 50 mM HEPES, 10 mM DTT, pH 7.5
- Recombinant Human PRL-3/PTP4A3 (rhPTP4A3) (Catalog # 8455-PT)
- Substrate: p-Nitrophenyl phosphate (Sigma, Catalog # N2765), 10 mM stock in deionized water
- NaOH, 0.2 M in deionized water
- 96-well Clear Plate (Catalog # DY990)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhPTP4A3 to 40 µg/mL in Assay buffer.
- Dilute Substrate to 5 mM in Assay buffer.
- Prepare reaction mixtures by combining equivalent volumes of dilute rhPTP4A3 and dilute Substrate in microtubes. Include an Enzyme Control by combining dilute rhPTP4A3 with twice the volume of 0.2 M NaOH, mix briefly, then add a volume of dilute Substrate equivalent to the volume of rhPTP4A3. The Enzyme Control will have 2x the volume of the reaction mixture.
- Incubate Reactions and Enzyme Controls at 37 °C for 24 hours.
- Load 100 µL of Reactions into a plate in triplicate and stop the reactions by adding 100 µL 0.2 M NaOH.
- Load 200 µL of Enzyme Controls into plate in triplicate.
- Read plate at 410 nm (absorbance) in endpoint mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Abs* (OD) x Conversion Factor** (pmol/OD) |
| Incubation time (min) x amount of enzyme (µg) |
*Adjusted for Enzyme Controls.
**Derived using calibration standard p-Nitrophenol (Sigma, Catalog # 241326).
Per Well:
- rhPTP4A3: 2 µg
- pNPP: 1.25 mM
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