Recombinant Human Pro-Collagen II Protein, CF

R&D Systems | Catalog # 7589-CL

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human Pro-Collagen II Protein (7589-CL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P02458

Applications

Enzyme Activity
View all Collagen II Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Collagen II protein
Gln26-Arg266 & Gly957-Leu1487, with an N-terminal 5-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His & Asp1242

Predicted Molecular Mass

78 kDa

SDS-PAGE

92-100 kDa, 65-70 kDa, 36 kDa, reducing conditions

Activity

Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN).
>50% of full-length Pro-Collagen II is cleaved by Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN), as measured under the described conditions.

Formulation, Preparation, and Storage

7589-CL
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Collagen II

Type II collagen is an essential component of cartilage. It is essential for skeletal development and for the ability of cartilage to resist compressive forces. Type II collagen is a homotrimer of a1(II) chains. Defects in Type II collagen are the cause of numerous diseases, including chondrodysplasias and arthritis (1). This recombinant mini pro-a1(II) collagen consists of the N‑terminal propeptide, 108 Gly-X-Y repeats, and the C‑terminal propeptide. Proteolytic processing of the C‑terminal propeptide by the procollagen C-proteinase (BMP-1) releases chondrocalcin, a calcium binding protein.

References

  1. Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Entrez Gene IDs

1280 (Human); 12824 (Mouse); 25412 (Rat)

Gene Symbol

COL2A1

UniProt

P02458 (Human)

Additional Collagen II Products

Product Documents for Recombinant Human Pro-Collagen II Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Pro-Collagen II Protein, CF

For research use only

Citations for Recombinant Human Pro-Collagen II Protein, CF

Customer Reviews for Recombinant Human Pro-Collagen II Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Pro-Collagen II Protein, CF and earn rewards!

Have you used Recombinant Human Pro-Collagen II Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human Pro-Collagen II Protein, CF (7589-CL):

Materials
  • Dilution Buffer: 50 mM Tris, 5 mM CaCl2, pH 7.5
  • Assay Buffer: 50 mM Tris, 5 mM CaCl2, 150 mM NaCl, pH 7.5 (TCN)
  • Recombinant Human Pro-Collagen II (rhCOL-2A1) (Catalog # 7589-CL)
  • Recombinant Human BMP‑1/PCP (rhBMP-1) (Catalog # 1927-ZN)
  • Reducing SDS-PAGE Sample Buffer
  • SDS-PAGE or Western Blot
  1. Dilute rhCOL-2A1 to 75 μg/mL in Dilution Buffer.
  2. Dilute rhBMP-1 to 10 μg/mL in Assay Buffer.
  3. Combine one volume of diluted rhCOL-2A1 with three volumes of diluted rhBMP-1. For controls, combine one volume of rhCOL-2A1 with three volumes of Assay Buffer, as well as three volumes of rhBMP-1 with one volume of Assay Buffer.
  4. Incubate reaction vials and controls at 37 °C for one hour.
  5. After incubation, combine rhCOL-2A1/rhBMP-1 reaction mixtures and controls with reducing SDS-PAGE gel buffer at a 2:1 (reaction mixture:gel buffer) ratio (v/v). Mix and incubate samples at 95-100 °C for 3-5 minutes to stop reactions.
  6. Load 40 μL (0.5 μg of rhCOL-2A1) per lane and analyze the cleavage by SDS-PAGE followed by protein staining and/or Western blot.
  7. Activity calculation:

  % Cleavage = [1  -  

% full-length rhCOL-2A1 (reaction) ] x 100%
% full-length rhCOL-2A1 (control)

Per Lane:

  • rhCOL-2A1: 0.5 µg
  • rhBMP-1: 0.2 µg

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes